Search results for the GEO ID: GSE13274  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM335165 | GPL570 |
|
Quiescent HMEC A2
|
Quiescent Ad-GFP infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335165
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335165/suppl/GSM335165.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335166 | GPL570 |
|
Quiescent HMEC A4
|
Quiescent Ad-GFP infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335166
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335166/suppl/GSM335166.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335167 | GPL570 |
|
Quiescent HMEC A5
|
Quiescent Ad-GFP infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335167
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335167/suppl/GSM335167.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335168 | GPL570 |
|
Quiescent HMEC A9
|
Quiescent Ad-GFP infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335168
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335168/suppl/GSM335168.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335169 | GPL570 |
|
Quiescent HMEC A10
|
Quiescent Ad-GFP infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335169
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335169/suppl/GSM335169.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335170 | GPL570 |
|
Quiescent HMEC B4
|
Quiescent Ad-HER2wt infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335170
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335170/suppl/GSM335170.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335171 | GPL570 |
|
Quiescent HMEC B5
|
Quiescent Ad-HER2wt infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335171
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335171/suppl/GSM335171.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335172 | GPL570 |
|
Quiescent HMEC B8
|
Quiescent Ad-HER2wt infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335172
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335172/suppl/GSM335172.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335173 | GPL570 |
|
Quiescent HMEC B9
|
Quiescent Ad-HER2wt infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335173
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335173/suppl/GSM335173.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335174 | GPL570 |
|
Quiescent HMEC B10
|
Quiescent Ad-HER2wt infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335174
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335174/suppl/GSM335174.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335175 | GPL570 |
|
Quiescent HMEC C2
|
Quiescent Ad-HER2-ki infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335175
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335175/suppl/GSM335175.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335176 | GPL570 |
|
Quiescent HMEC C3
|
Quiescent Ad-HER2-ki infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335176
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335176/suppl/GSM335176.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335177 | GPL570 |
|
Quiescent HMEC C4
|
Quiescent Ad-HER2-ki infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335177
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335177/suppl/GSM335177.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335178 | GPL570 |
|
Quiescent HMEC C9
|
Quiescent Ad-HER2-ki infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335178
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335178/suppl/GSM335178.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
GSM335179 | GPL570 |
|
Quiescent HMEC C10
|
Quiescent Ad-HER2-ki infected HMECs
|
HMECs
passage 3
serum starved for 36 hrs.
Infected for 18 hrs.
|
Quiescent HMECs were infected for 18hrs. RNA was then harvested and hybridized to a HU-133+ v2 array.
|
| Sample_geo_accession | GSM335179
| | Sample_status | Public on Nov 15 2008
| | Sample_submission_date | Oct 19 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Human Mammary epithelial cells cultured from normal donors undergoing routine reduction mammoplasties.
| | Sample_treatment_protocol_ch1 | Primary HMECs prepared from normal donors undergoing reduction mammoplasties were passaged 2 times and serum starved for 36 hrs.
| | Sample_treatment_protocol_ch1 | Infected with a 1st Generation E1-E3- GFP (under CMV promoter) Adenovirus
| | Sample_treatment_protocol_ch1 | RNA harvested 18 hours post infection
| | Sample_growth_protocol_ch1 | Used standard HMEC culturing techniques (Clonetics media with growth additives)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | We generated biotin-labeled cRNA by in vitro transcription from the DNA.
| | Sample_hyb_protocol | The cRNA was fragmented before hybridization, and we prepared a hybridization cocktail that included the fragmented cRNA, probe array controls, bovine serum albumin and herring sperm DNA. We hybridized the cRNA to the oligonucleotide probes on the probe array for a 16-h incubation at 45 °C. Immediately after the hybridization, the hybridized probe array underwent an automated washing and staining protocol on an Affymetrix fluidics station.
| | Sample_scan_protocol | We scanned the DNA chips with the Affymetrix GeneChip scanner and processed the signals by the GeneChip expression analysis algorithm (v.2; Affymetrix).
| | Sample_data_processing | MAS 5.0 Expression Analysis
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zachary,Conrad,Hartman
| | Sample_contact_email | zch@duke.edu
| | Sample_contact_phone | 919-684-9197
| | Sample_contact_laboratory | Lyerly Lab
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Duke University
| | Sample_contact_address | Research Drive MSRB rm 417
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335179/suppl/GSM335179.CEL.gz
| | Sample_series_id | GSE13274
| | Sample_data_row_count | 54675
| | |
|
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