Search results for the GEO ID: GSE13333 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM335971 | GPL1261 |
|
Srf control_1
|
Srf loxP/+ AlfpCre male liver; control sample
|
Srf loxP/+ AlfpCre male liver
3 weeks old
control sample
|
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE) of genes that are expressed in response to mitogens. SRF plays essential roles in muscle and nervous system development; however, little is known about the role of SRF during liver growth and function. To examine the function of SRF in the liver, we generated mice in which the Srf gene was specifically disrupted in hepatocytes. The survival of mice lacking hepatic SRF activity was lower than that of control mice; moreover, surviving mutant mice were smaller and had lower blood glucose and triglyceride levels compared with control mice. Srf-deficient livers were also smaller than control livers, hepatocyte morphology was abnormal, and liver-cell proliferation and viability was compromised. Gene array and quantitative RT-PCR analysis of SRF depleted livers revealed a reduction in mRNAs encoding components of the growth hormone/IGF1 pathway, cyclins, several metabolic regulators, and cytochrome p450 enzymes. Conclusion: SRF is essential for hepatocyte proliferation and survival, liver function, and control of postnatal body growth by regulating hepatocyte gene expression.
|
Sample_geo_accession | GSM335971
| Sample_status | Public on Mar 01 2009
| Sample_submission_date | Oct 22 2008
| Sample_last_update_date | Oct 29 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy mini kit (qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix one-cycle target labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard GCOS data processing produced the values provided.
| Sample_platform_id | GPL1261
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335971/suppl/GSM335971.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335971/suppl/GSM335971.CHP.gz
| Sample_series_id | GSE13333
| Sample_data_row_count | 45101
| |
|
GSM336405 | GPL1261 |
|
Srf control_2
|
Srf loxP/+ AlfpCre male liver; control sample
|
Srf loxP/+ AlfpCre male liver
3 weeks old
control sample
|
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE) of genes that are expressed in response to mitogens. SRF plays essential roles in muscle and nervous system development; however, little is known about the role of SRF during liver growth and function. To examine the function of SRF in the liver, we generated mice in which the Srf gene was specifically disrupted in hepatocytes. The survival of mice lacking hepatic SRF activity was lower than that of control mice; moreover, surviving mutant mice were smaller and had lower blood glucose and triglyceride levels compared with control mice. Srf-deficient livers were also smaller than control livers, hepatocyte morphology was abnormal, and liver-cell proliferation and viability was compromised. Gene array and quantitative RT-PCR analysis of SRF depleted livers revealed a reduction in mRNAs encoding components of the growth hormone/IGF1 pathway, cyclins, several metabolic regulators, and cytochrome p450 enzymes. Conclusion: SRF is essential for hepatocyte proliferation and survival, liver function, and control of postnatal body growth by regulating hepatocyte gene expression.
|
Sample_geo_accession | GSM336405
| Sample_status | Public on Mar 01 2009
| Sample_submission_date | Oct 23 2008
| Sample_last_update_date | Oct 29 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy mini kit (qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix one-cycle target labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard GCOS data processing produced the values provided.
| Sample_platform_id | GPL1261
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336405/suppl/GSM336405.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336405/suppl/GSM336405.CHP.gz
| Sample_series_id | GSE13333
| Sample_data_row_count | 45101
| |
|
GSM336406 | GPL1261 |
|
Srf control_3
|
Srf loxP/+ AlfpCre male liver; control sample
|
Srf loxP/+ AlfpCre male liver
3 weeks old
control sample
|
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE) of genes that are expressed in response to mitogens. SRF plays essential roles in muscle and nervous system development; however, little is known about the role of SRF during liver growth and function. To examine the function of SRF in the liver, we generated mice in which the Srf gene was specifically disrupted in hepatocytes. The survival of mice lacking hepatic SRF activity was lower than that of control mice; moreover, surviving mutant mice were smaller and had lower blood glucose and triglyceride levels compared with control mice. Srf-deficient livers were also smaller than control livers, hepatocyte morphology was abnormal, and liver-cell proliferation and viability was compromised. Gene array and quantitative RT-PCR analysis of SRF depleted livers revealed a reduction in mRNAs encoding components of the growth hormone/IGF1 pathway, cyclins, several metabolic regulators, and cytochrome p450 enzymes. Conclusion: SRF is essential for hepatocyte proliferation and survival, liver function, and control of postnatal body growth by regulating hepatocyte gene expression.
|
Sample_geo_accession | GSM336406
| Sample_status | Public on Mar 01 2009
| Sample_submission_date | Oct 23 2008
| Sample_last_update_date | Oct 29 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy mini kit (qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix one-cycle target labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard GCOS data processing produced the values provided.
| Sample_platform_id | GPL1261
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336406/suppl/GSM336406.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336406/suppl/GSM336406.CHP.gz
| Sample_series_id | GSE13333
| Sample_data_row_count | 45101
| |
|
GSM336407 | GPL1261 |
|
Srf mutant_1
|
Srf loxP/loxP AlfpCre male liver; mutant sample
|
Srf loxP/loxP AlfpCre male liver
3 weeks old
mutant sample
|
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE) of genes that are expressed in response to mitogens. SRF plays essential roles in muscle and nervous system development; however, little is known about the role of SRF during liver growth and function. To examine the function of SRF in the liver, we generated mice in which the Srf gene was specifically disrupted in hepatocytes. The survival of mice lacking hepatic SRF activity was lower than that of control mice; moreover, surviving mutant mice were smaller and had lower blood glucose and triglyceride levels compared with control mice. Srf-deficient livers were also smaller than control livers, hepatocyte morphology was abnormal, and liver-cell proliferation and viability was compromised. Gene array and quantitative RT-PCR analysis of SRF depleted livers revealed a reduction in mRNAs encoding components of the growth hormone/IGF1 pathway, cyclins, several metabolic regulators, and cytochrome p450 enzymes. Conclusion: SRF is essential for hepatocyte proliferation and survival, liver function, and control of postnatal body growth by regulating hepatocyte gene expression.
|
Sample_geo_accession | GSM336407
| Sample_status | Public on Mar 01 2009
| Sample_submission_date | Oct 23 2008
| Sample_last_update_date | Oct 29 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy mini kit (qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix one-cycle target labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard GCOS data processing produced the values provided.
| Sample_platform_id | GPL1261
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336407/suppl/GSM336407.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336407/suppl/GSM336407.CHP.gz
| Sample_series_id | GSE13333
| Sample_data_row_count | 45101
| |
|
GSM336408 | GPL1261 |
|
Srf mutant_2
|
Srf loxP/loxP AlfpCre male liver; mutant sample
|
Srf loxP/loxP AlfpCre male liver
3 weeks old
mutant sample
|
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE) of genes that are expressed in response to mitogens. SRF plays essential roles in muscle and nervous system development; however, little is known about the role of SRF during liver growth and function. To examine the function of SRF in the liver, we generated mice in which the Srf gene was specifically disrupted in hepatocytes. The survival of mice lacking hepatic SRF activity was lower than that of control mice; moreover, surviving mutant mice were smaller and had lower blood glucose and triglyceride levels compared with control mice. Srf-deficient livers were also smaller than control livers, hepatocyte morphology was abnormal, and liver-cell proliferation and viability was compromised. Gene array and quantitative RT-PCR analysis of SRF depleted livers revealed a reduction in mRNAs encoding components of the growth hormone/IGF1 pathway, cyclins, several metabolic regulators, and cytochrome p450 enzymes. Conclusion: SRF is essential for hepatocyte proliferation and survival, liver function, and control of postnatal body growth by regulating hepatocyte gene expression.
|
Sample_geo_accession | GSM336408
| Sample_status | Public on Mar 01 2009
| Sample_submission_date | Oct 23 2008
| Sample_last_update_date | Oct 29 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy mini kit (qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix one-cycle target labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard GCOS data processing produced the values provided.
| Sample_platform_id | GPL1261
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336408/suppl/GSM336408.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336408/suppl/GSM336408.CHP.gz
| Sample_series_id | GSE13333
| Sample_data_row_count | 45101
| |
|
GSM336409 | GPL1261 |
|
Srf mutant_3
|
Srf loxP/loxP AlfpCre male liver; mutant sample
|
Srf loxP/loxP AlfpCre male liver
3 weeks old
mutant sample
|
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE) of genes that are expressed in response to mitogens. SRF plays essential roles in muscle and nervous system development; however, little is known about the role of SRF during liver growth and function. To examine the function of SRF in the liver, we generated mice in which the Srf gene was specifically disrupted in hepatocytes. The survival of mice lacking hepatic SRF activity was lower than that of control mice; moreover, surviving mutant mice were smaller and had lower blood glucose and triglyceride levels compared with control mice. Srf-deficient livers were also smaller than control livers, hepatocyte morphology was abnormal, and liver-cell proliferation and viability was compromised. Gene array and quantitative RT-PCR analysis of SRF depleted livers revealed a reduction in mRNAs encoding components of the growth hormone/IGF1 pathway, cyclins, several metabolic regulators, and cytochrome p450 enzymes. Conclusion: SRF is essential for hepatocyte proliferation and survival, liver function, and control of postnatal body growth by regulating hepatocyte gene expression.
|
Sample_geo_accession | GSM336409
| Sample_status | Public on Mar 01 2009
| Sample_submission_date | Oct 23 2008
| Sample_last_update_date | Oct 29 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy mini kit (qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix one-cycle target labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard GCOS data processing produced the values provided.
| Sample_platform_id | GPL1261
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336409/suppl/GSM336409.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336409/suppl/GSM336409.CHP.gz
| Sample_series_id | GSE13333
| Sample_data_row_count | 45101
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|