Search results for the GEO ID: GSE13400 ![](/q11.jpeg) |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM312550 | GPL570 |
|
HET-1A_REST 4Hr_1
|
HET-1A cells in untreated media for 4 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM312550
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Aug 14 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | HET-1A are avliable from the ATCC biobank
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labelled as per instructions by affymetrix.
| Sample_hyb_protocol | Hybridised as per instructions by affymetrix.
| Sample_scan_protocol | Scanned as per instructions by affymetrix at tgt 100.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312550/suppl/GSM312550.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312550/suppl/GSM312550.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM313007 | GPL570 |
|
HET-1A_REST 4Hr_2
|
HET-1A cells in untreated media for 4 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM313007
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Aug 15 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labelled as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313007/suppl/GSM313007.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313007/suppl/GSM313007.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM313008 | GPL570 |
|
HET-1A_REST_8HR_1
|
HET-1A cells in untreated media for 8 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM313008
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Aug 15 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labelled as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313008/suppl/GSM313008.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313008/suppl/GSM313008.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM313009 | GPL570 |
|
HET-1A_REST_8Hr_2
|
HET-1A cells in untreated media for 8 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM313009
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Aug 15 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labelled as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313009/suppl/GSM313009.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313009/suppl/GSM313009.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM318847 | GPL570 |
|
HET-1A_REST 12Hr_1
|
HET-1A cells in untreated media for 12 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM318847
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Sep 09 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318847/suppl/GSM318847.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318847/suppl/GSM318847.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM318857 | GPL570 |
|
HET-1A_REST 12Hr_2
|
HET-1A cells in untreated media for 12 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM318857
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Sep 09 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318857/suppl/GSM318857.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318857/suppl/GSM318857.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM318858 | GPL570 |
|
HET-1A_REST 24Hr_1
|
HET-1A cells in untreated media for 24 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM318858
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Sep 09 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318858/suppl/GSM318858.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318858/suppl/GSM318858.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM318860 | GPL570 |
|
HET-1A_REST 24Hr_2
|
HET-1A cells in untreated media for 24 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM318860
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Sep 09 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318860/suppl/GSM318860.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318860/suppl/GSM318860.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM336627 | GPL570 |
|
HET-1A_DCA_24Hr_1
|
HET-1A cells exposed to 300um DCA for 24 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM336627
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 24 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed to DCA 300um in media without supplementation.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336627/suppl/GSM336627.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336627/suppl/GSM336627.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM336629 | GPL570 |
|
HET-1A_DCA_24Hr_2
|
HET-1A cells exposed to 300um DCA for 24 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM336629
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 24 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed to DCA 300um in media without supplementation.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336629/suppl/GSM336629.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336629/suppl/GSM336629.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM336630 | GPL570 |
|
HET-1A_DCA_12Hr_1
|
HET-1A cells exposed to 300um DCA for 12 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM336630
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 24 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed to DCA 300um in media without supplementation.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336630/suppl/GSM336630.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336630/suppl/GSM336630.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM336631 | GPL570 |
|
HET-1A_DCA_12Hr_2
|
HET-1A cells exposed to 300um DCA for 12 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM336631
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 24 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed to DCA 300um in media without supplementation.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336631/suppl/GSM336631.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336631/suppl/GSM336631.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM336632 | GPL570 |
|
HET-1A_DCA_8Hr_1
|
HET-1A cells exposed to 300um DCA for 8 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM336632
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 24 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed to DCA 300um in media without supplementation.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336632/suppl/GSM336632.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336632/suppl/GSM336632.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM336633 | GPL570 |
|
HET-1A_DCA_8Hr_2
|
HET-1A cells exposed to 300um DCA for 8 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM336633
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 24 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed to DCA 300um in media without supplementation.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336633/suppl/GSM336633.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336633/suppl/GSM336633.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM336634 | GPL570 |
|
HET-1A_DCA_4Hr_1
|
HET-1A cells exposed to 300um DCA for 4 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM336634
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 24 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed to DCA 300um in media without supplementation.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336634/suppl/GSM336634.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336634/suppl/GSM336634.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM336635 | GPL570 |
|
HET-1A_DCA_4Hr_2
|
HET-1A cells exposed to 300um DCA for 4 hours
|
The HET-1A cell line is derived from the normal squamous esophageal epithelium and used in these experiments at passage 4.
|
HET-1A cells were exposed to 300um DCA over 24 hours in duplicate experiments including matched timepoint controls
|
Sample_geo_accession | GSM336635
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 24 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were exposed to DCA 300um in media without supplementation.
| Sample_growth_protocol_ch1 | HET-1A cells are grown in bronchial epithelial base medium (BEBM) with supplementation as described by the American Tissue Culture Collection (ATCC).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value data was derived through transformations in GCOS/MAS5 at a target intensity of 100
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336635/suppl/GSM336635.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM336nnn/GSM336635/suppl/GSM336635.CHP.gz
| Sample_series_id | GSE13378
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337722 | GPL570 |
|
SKGT4_REST_4Hr_1
|
SKGT4 cells in untreated media for 4 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337722
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337722/suppl/GSM337722.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337722/suppl/GSM337722.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337723 | GPL570 |
|
SKGT4_REST_4Hr_2
|
SKGT4 cells in untreated media for 4 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337723
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337723/suppl/GSM337723.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337723/suppl/GSM337723.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337724 | GPL570 |
|
SKGT4_REST_8Hr_1
|
SKGT4 cells in untreated media for 8 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337724
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337724/suppl/GSM337724.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337724/suppl/GSM337724.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337725 | GPL570 |
|
SKGT4_REST_8Hr_2
|
SKGT4 cells in untreated media for 8 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337725
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337725/suppl/GSM337725.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337725/suppl/GSM337725.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337726 | GPL570 |
|
SKGT4_REST_12Hr_1
|
SKGT4 cells in untreated media for 12 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337726
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337726/suppl/GSM337726.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337726/suppl/GSM337726.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337727 | GPL570 |
|
SJGT4_REST_12Hr_2
|
SKGT4 cells in untreated media for 12 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337727
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337727/suppl/GSM337727.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337727/suppl/GSM337727.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337730 | GPL570 |
|
SKGT4_REST_24Hr_1
|
SKGT4 cells in untreated media for 24 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337730
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337730/suppl/GSM337730.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337730/suppl/GSM337730.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337732 | GPL570 |
|
SKGT4_REST_24Hr_2
|
SKGT4 cells in untreated media for 24 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337732
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have not been treated and are used as a biological time point control for experiments involving exposure of these cells to carcinogens over time.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337732/suppl/GSM337732.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337732/suppl/GSM337732.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337733 | GPL570 |
|
SKGT4_DCA_4Hr_1
|
SKGT4 cells exposed to DCA (300um) media for 4 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337733
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have been exposed to deoxycholic acid (DCA) 300um for the time detailed above, in RPMI media without supplementation or fetal calf serum.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337733/suppl/GSM337733.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337733/suppl/GSM337733.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337735 | GPL570 |
|
SKGT4_DCA_8Hr_1
|
SKGT4 cells exposed to DCA (300um) media for 8 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337735
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have been exposed to deoxycholic acid (DCA) 300um for the time detailed above, in RPMI media without supplementation or fetal calf serum.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337735/suppl/GSM337735.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337735/suppl/GSM337735.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337736 | GPL570 |
|
SKGT4_DCA_8Hr_2
|
SKGT4 cells exposed to DCA (300um) media for 8 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337736
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have been exposed to deoxycholic acid (DCA) 300um for the time detailed above, in RPMI media without supplementation or fetal calf serum.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337736/suppl/GSM337736.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337736/suppl/GSM337736.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337737 | GPL570 |
|
SKGT4_DCA_12Hr_1
|
SKGT4 cells exposed to DCA (300um) media for 12 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337737
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have been exposed to deoxycholic acid (DCA) 300um for the time detailed above, in RPMI media without supplementation or fetal calf serum.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337737/suppl/GSM337737.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337737/suppl/GSM337737.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337738 | GPL570 |
|
SKGT4_DCA_12Hr_2
|
SKGT4 cells exposed to DCA (300um) media for 12 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337738
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have been exposed to deoxycholic acid (DCA) 300um for the time detailed above, in RPMI media without supplementation or fetal calf serum.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337738/suppl/GSM337738.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337738/suppl/GSM337738.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337739 | GPL570 |
|
SKGT4_DCA_24Hr_1
|
SKGT4 cells exposed to DCA (300um) media for 24 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337739
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have been exposed to deoxycholic acid (DCA) 300um for the time detailed above, in RPMI media without supplementation or fetal calf serum.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337739/suppl/GSM337739.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337739/suppl/GSM337739.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
GSM337740 | GPL570 |
|
SKGT4_DCA_24Hr_2
|
SKGT4 cells exposed to DCA (300um) media for 24 hours
|
The SKGT4 cell line is derived from the Barrett's associated adenocarcinoma of the lower esophageal epithelium and used in these experiments at passage 4.
|
RNA samples were taken at 4, 8, 12 and 24 hours from DCA (DCA) exposed and resting (REST) timepoint controls.
|
Sample_geo_accession | GSM337740
| Sample_status | Public on Feb 20 2010
| Sample_submission_date | Oct 28 2008
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | These cells have been exposed to deoxycholic acid (DCA) 300um for the time detailed above, in RPMI media without supplementation or fetal calf serum.
| Sample_growth_protocol_ch1 | SKGT4 cells are grown in RPMI1640 with 10% fetal calf serum and penn-step-glutamine supplementation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Machery-Nagel RNA extraction spin columns.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | as per instructions by affymetrix.
| Sample_hyb_protocol | as per instructions by affymetrix.
| Sample_scan_protocol | as per instructions by affymetrix.
| Sample_data_processing | Value as determined at a target intensity of 100 in MAS5.0
| Sample_platform_id | GPL570
| Sample_contact_name | Shane,P,Duggan
| Sample_contact_email | shduggan@tcd.ie
| Sample_contact_phone | 0035318963273
| Sample_contact_fax | 0035318963503
| Sample_contact_laboratory | Rm 1.16
| Sample_contact_department | Clinical Medicine
| Sample_contact_institute | Institute of Molecular Medicine
| Sample_contact_address | TCD Health Sciences Building, St James Hospital,
| Sample_contact_city | Dublin
| Sample_contact_zip/postal_code | Dublin 8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337740/suppl/GSM337740.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM337nnn/GSM337740/suppl/GSM337740.CHP.gz
| Sample_series_id | GSE13376
| Sample_series_id | GSE13400
| Sample_data_row_count | 54675
| |
|
|
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Make groups for comparisons ![](/q11.jpeg) |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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