Search results for the GEO ID: GSE13535  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM341273 | GPL1355 |
|
2 hour vehicle control rat, lung tissue, biological rep1
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of vehicle treatment.
|
| Sample_geo_accession | GSM341273
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after vehicle treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341273/suppl/GSM341273.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341273/suppl/GSM341273.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341274 | GPL1355 |
|
2 hour vehicle control rat, lung tissue, biological rep2
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of vehicle treatment.
|
| Sample_geo_accession | GSM341274
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after vehicle treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341274/suppl/GSM341274.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341274/suppl/GSM341274.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341275 | GPL1355 |
|
2 hour vehicle control rat, lung tissue, biological rep3
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of vehicle treatment.
|
| Sample_geo_accession | GSM341275
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after vehicle treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341275/suppl/GSM341275.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341275/suppl/GSM341275.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341276 | GPL1355 |
|
2 hour PE1.3 embolized rat, lung tissue, biological rep1
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of PE1.3 treatment.
|
| Sample_geo_accession | GSM341276
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341276/suppl/GSM341276.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341276/suppl/GSM341276.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341277 | GPL1355 |
|
2 hour PE1.3 embolized rat, lung tissue, biological rep2
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of PE1.3 treatment.
|
| Sample_geo_accession | GSM341277
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341277/suppl/GSM341277.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341277/suppl/GSM341277.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341278 | GPL1355 |
|
2 hour PE1.3 embolized rat, lung tissue, biological rep3
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of PE1.3 treatment.
|
| Sample_geo_accession | GSM341278
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341278/suppl/GSM341278.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341278/suppl/GSM341278.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341279 | GPL1355 |
|
2 hour PE1.3 embolized rat, lung tissue, biological rep4
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of PE1.3 treatment.
|
| Sample_geo_accession | GSM341279
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341279/suppl/GSM341279.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341279/suppl/GSM341279.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341280 | GPL1355 |
|
2 hour PE2.0 embolized rat, lung tissue, biological rep1
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of PE2.0 treatment.
|
| Sample_geo_accession | GSM341280
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE2.0 treatment; 2.0 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after PE2.0 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341280/suppl/GSM341280.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341280/suppl/GSM341280.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341281 | GPL1355 |
|
2 hour PE2.0 embolized rat, lung tissue, biological rep2
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of PE2.0 treatment.
|
| Sample_geo_accession | GSM341281
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE2.0 treatment; 2.0 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after PE2.0 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341281/suppl/GSM341281.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341281/suppl/GSM341281.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341282 | GPL1355 |
|
2 hour PE2.0 embolized rat, lung tissue, biological rep3
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of PE2.0 treatment.
|
| Sample_geo_accession | GSM341282
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE2.0 treatment; 2.0 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after PE2.0 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341282/suppl/GSM341282.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341282/suppl/GSM341282.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341283 | GPL1355 |
|
2 hour PE2.0 embolized rat, lung tissue, biological rep4
|
Total lung tissue 2 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 2 hours of PE2.0 treatment.
|
| Sample_geo_accession | GSM341283
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE2.0 treatment; 2.0 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 2 hours after PE2.0 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341283/suppl/GSM341283.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341283/suppl/GSM341283.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341284 | GPL1355 |
|
18 hour vehicle control rat, lung tissue, biological rep1
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of vehicle treatment.
|
| Sample_geo_accession | GSM341284
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after vehicle treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341284/suppl/GSM341284.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341284/suppl/GSM341284.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341285 | GPL1355 |
|
18 hour vehicle control rat, lung tissue, biological rep2
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of vehicle treatment.
|
| Sample_geo_accession | GSM341285
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after vehicle treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341285/suppl/GSM341285.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341285/suppl/GSM341285.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341286 | GPL1355 |
|
18 hour vehicle control rat, lung tissue, biological rep3
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of vehicle treatment.
|
| Sample_geo_accession | GSM341286
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after vehicle treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341286/suppl/GSM341286.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341286/suppl/GSM341286.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341287 | GPL1355 |
|
18 hour PE1.3 embolized rat, lung tissue, biological rep1
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of PE1.3 treatment.
|
| Sample_geo_accession | GSM341287
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341287/suppl/GSM341287.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341287/suppl/GSM341287.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341288 | GPL1355 |
|
18 hour PE1.3 embolized rat, lung tissue, biological rep2
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of PE1.3 treatment.
|
| Sample_geo_accession | GSM341288
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341288/suppl/GSM341288.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341288/suppl/GSM341288.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341289 | GPL1355 |
|
18 hour PE1.3 embolized rat, lung tissue, biological rep3
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of PE1.3 treatment.
|
| Sample_geo_accession | GSM341289
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341289/suppl/GSM341289.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341289/suppl/GSM341289.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341290 | GPL1355 |
|
18 hour PE1.3 embolized rat, lung tissue, biological rep4
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of PE1.3 treatment.
|
| Sample_geo_accession | GSM341290
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.10 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE1.3 treatment; 1.3 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after PE1.3 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341290/suppl/GSM341290.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341290/suppl/GSM341290.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341291 | GPL1355 |
|
18 hour PE2.0 embolized rat, lung tissue, biological rep1
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of PE2.0 treatment.
|
| Sample_geo_accession | GSM341291
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE2.0 treatment; 2.0 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after PE2.0 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341291/suppl/GSM341291.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341291/suppl/GSM341291.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341292 | GPL1355 |
|
18 hour PE2.0 embolized rat, lung tissue, biological rep2
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of PE2.0 treatment.
|
| Sample_geo_accession | GSM341292
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE2.0 treatment; 2.0 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after PE2.0 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341292/suppl/GSM341292.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341292/suppl/GSM341292.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341293 | GPL1355 |
|
18 hour PE2.0 embolized rat, lung tissue, biological rep3
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of PE2.0 treatment.
|
| Sample_geo_accession | GSM341293
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE2.0 treatment; 2.0 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after PE2.0 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341293/suppl/GSM341293.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341293/suppl/GSM341293.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
|
GSM341294 | GPL1355 |
|
18 hour PE2.0 embolized rat, lung tissue, biological rep4
|
Total lung tissue 18 hours after treatment
|
Sprague Dawley male
Weight: approximately 350 grams at time of experiment
|
Gene expression data from total lung RNA after 18 hours of PE2.0 treatment.
|
| Sample_geo_accession | GSM341294
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Nov 08 2008
| | Sample_last_update_date | Mar 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of a 10% W/V suspension of 25 micron polystyrene mirospheres in 0.01% Tween-20 as PE2.0 treatment; 2.0 million microspheres/100 gram weight
| | Sample_growth_protocol_ch1 | Rat was euthanized 18 hours after PE2.0 treatment. Lungs were excised surgically after bronchoalveloar lavage with 8 ml saline
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Lung tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341294/suppl/GSM341294.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341294/suppl/GSM341294.CHP.gz
| | Sample_series_id | GSE13535
| | Sample_data_row_count | 31099
| | |
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