Search results for the GEO ID: GSE13537  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM341298 | GPL1355 |
|
Control, 0 hrs KCl, Biol Rep1
|
Hippocampal neurons, control shRNAs, 0 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341298
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341298/suppl/GSM341298.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341299 | GPL1355 |
|
Control, 1 hrs KCl, Biol Rep1
|
Hippocampal neurons, control shRNAs, 1 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341299
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341299/suppl/GSM341299.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341300 | GPL1355 |
|
Control, 6 hrs KCl, Biol Rep1
|
Hippocampal neurons, control shRNAs, 6 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341300
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341300/suppl/GSM341300.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341301 | GPL1355 |
|
MEF2 RNAi, 0 hrs KCl, Biol Rep1
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 0 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341301
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341301/suppl/GSM341301.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341302 | GPL1355 |
|
MEF2 RNAi, 1 hrs KCl, Biol Rep1
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 1 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341302
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341302/suppl/GSM341302.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341303 | GPL1355 |
|
MEF2 RNAi, 6 hrs KCl, Biol Rep1
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 6 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341303
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341303/suppl/GSM341303.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341304 | GPL1355 |
|
Control, 0 hrs KCl, Biol Rep2
|
Hippocampal neurons, control shRNAs, 0 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341304
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341304/suppl/GSM341304.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341305 | GPL1355 |
|
Control, 1 hrs KCl, Biol Rep2
|
Hippocampal neurons, control shRNAs, 1 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341305
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341305/suppl/GSM341305.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341306 | GPL1355 |
|
Control, 6 hrs KCl, Biol Rep2
|
Hippocampal neurons, control shRNAs, 6 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341306
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341306/suppl/GSM341306.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341307 | GPL1355 |
|
MEF2 RNAi, 0 hrs KCl, Biol Rep2
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 0 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341307
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341307/suppl/GSM341307.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341308 | GPL1355 |
|
MEF2 RNAi, 1 hrs KCl, Biol Rep2
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 1 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341308
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341308/suppl/GSM341308.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341309 | GPL1355 |
|
MEF2 RNAi, 6 hrs KCl, Biol Rep2
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 6 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341309
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341309/suppl/GSM341309.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341310 | GPL1355 |
|
Control, 0 hrs KCl, Biol Rep3
|
Hippocampal neurons, control shRNAs, 0 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341310
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341310/suppl/GSM341310.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341311 | GPL1355 |
|
Control, 1 hrs KCl, Biol Rep3
|
Hippocampal neurons, control shRNAs, 1 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341311
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341311/suppl/GSM341311.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341312 | GPL1355 |
|
Control, 6 hrs KCl, Biol Rep3
|
Hippocampal neurons, control shRNAs, 6 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341312
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341312/suppl/GSM341312.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341313 | GPL1355 |
|
MEF2 RNAi, 0 hrs KCl, Biol Rep3
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 0 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341313
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341313/suppl/GSM341313.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341314 | GPL1355 |
|
MEF2 RNAi, 1 hrs KCl, Biol Rep3
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 1 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341314
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341314/suppl/GSM341314.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
GSM341315 | GPL1355 |
|
MEF2 RNAi, 6 hrs KCl, Biol Rep3
|
Hippocampal neurons, MEF2A and MEF2D shRNAs, 6 hours KCl depolarization
|
n/a
|
Expression profiling in hippocampal neurons to identify activity-regulated genes controlled by MEF2
|
| Sample_geo_accession | GSM341315
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Jan 10 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | On day in vitro 3, neurons were transduced with lentiviruses expressing either control or MEF2-specific shRNAs. On day in vitro 10, AP5 and TTX were applied to neurons for one hour, after which 55mM extracellular KCl was applied for 0, 1 or 6 hours.
| | Sample_growth_protocol_ch1 | Hippocampal neurons were grown in dissociated culture for 10 days in vitro.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit from Qiagen
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled cRNA prepared according to standard Affymetrix procedures
| | Sample_hyb_protocol | Standard Affymetrix hybridization procedures.
| | Sample_scan_protocol | Standard Affymetrix scanning procedures.
| | Sample_data_processing | Raw CEL files were normalized using RMAexpress.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Steven,Willem,Flavell
| | Sample_contact_email | steven.flavell@tch.harvard.edu
| | Sample_contact_laboratory | Michael E. Greenberg
| | Sample_contact_department | Neurology
| | Sample_contact_institute | Children's Hospital Boston
| | Sample_contact_address | CLS 12260, 300 Longwood Avenue
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341315/suppl/GSM341315.CEL.gz
| | Sample_series_id | GSE13537
| | Sample_data_row_count | 31099
| | |
|
| |
| |
Make groups for comparisons |
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