Search results for the GEO ID: GSE13547  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM341374 | GPL1261 |
|
ZFXwt_B cell_0hr_rep1
|
ZFXwt B cell, a-IgM, 0 hr
|
strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341374
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341374/suppl/GSM341374.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341374/suppl/GSM341374.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341375 | GPL1261 |
|
ZFXwt_B cell_0hr_rep2
|
ZFXwt B cell, a-IgM, 0 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341375
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341375/suppl/GSM341375.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341375/suppl/GSM341375.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341376 | GPL1261 |
|
ZFXwt_B cell_2hr_rep1
|
ZFXwt B cell, a-IgM, 2 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341376
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341376/suppl/GSM341376.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341376/suppl/GSM341376.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341377 | GPL1261 |
|
ZFXwt_B cell_2hr_rep2
|
ZFXwt B cell, a-IgM, 2 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341377
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341377/suppl/GSM341377.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341377/suppl/GSM341377.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341378 | GPL1261 |
|
ZFXwt_B cell_12hr_rep1
|
ZFXwt B cell, a-IgM, 12 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341378
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341378/suppl/GSM341378.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341378/suppl/GSM341378.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341379 | GPL1261 |
|
ZFXwt_B cell_12hr_rep2
|
ZFXwt B cell, a-IgM, 12 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341379
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341379/suppl/GSM341379.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341379/suppl/GSM341379.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341380 | GPL1261 |
|
ZFXcko_B cell_0hr_rep1
|
ZFXcko B cell, a-IgM, 0 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341380
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341380/suppl/GSM341380.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341380/suppl/GSM341380.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341381 | GPL1261 |
|
ZFXcko_B cell_0hr_rep2
|
ZFXcko B cell, a-IgM, 0 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341381
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341381/suppl/GSM341381.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341381/suppl/GSM341381.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341382 | GPL1261 |
|
ZFXcko_B cell_2hr_rep1
|
ZFXcko B cell, a-IgM, 2 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341382
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341382/suppl/GSM341382.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341382/suppl/GSM341382.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341383 | GPL1261 |
|
ZFXcko_B cell_2hr_rep2
|
ZFXcko B cell, a-IgM, 2 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341383
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341383/suppl/GSM341383.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341383/suppl/GSM341383.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341384 | GPL1261 |
|
ZFXcko_B cell_12hr_rep1
|
ZFXcko B cell, a-IgM, 12 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341384
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341384/suppl/GSM341384.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341384/suppl/GSM341384.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
|
GSM341385 | GPL1261 |
|
ZFXcko_B cell_12hr_rep2
|
ZFXcko B cell, a-IgM, 12 hr
|
Strain: 129SvEv, Gender: Male, Age: 8wk, Cell type: Splenic follicular B cell
|
none
|
| Sample_geo_accession | GSM341385
| | Sample_status | Public on Apr 17 2009
| | Sample_submission_date | Nov 10 2008
| | Sample_last_update_date | Apr 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol (Invitrogen).
| | Sample_label_ch1 | Biotin-16-UTP, Biotin-11-CTP
| | Sample_label_protocol_ch1 | 100 ng of each RNA sample were used for reverse transcription and two rounds of linear antisense RNA amplification/labeling (Ambion). The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP(Roche Applied Sciences) and Biotin-11-CTP (PerkinElmer Life Sciences) in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
| | Sample_hyb_protocol | Labeled RNA samples (10 ug/array) were hybridized in duplicate to Mouse Genome 430 2.0 arrays (Affymetrix) at the Columbia University Microarray Project core facility according to the manufacturer's instructions.
| | Sample_scan_protocol | Array scanning was done using GCOS 1.4 software (Affymetrix).
| | Sample_data_processing | Raw data processing was done using GCOS 1.4 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Teresita,,Arenzana
| | Sample_contact_email | tla2004@columbia.edu
| | Sample_contact_laboratory | Boris Reizis
| | Sample_contact_department | Microbiology Department
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 701 W. 168th St., HHSC 609
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341385/suppl/GSM341385.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341385/suppl/GSM341385.CHP.gz
| | Sample_series_id | GSE13547
| | Sample_data_row_count | 45101
| | |
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