Result

Search results for the GEO ID: GSE13567

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM341571

GPL1261

Mock (clone 12)

Stably transfected NIH-3T3 cells

Mock transfected

Sample_geo_accession	GSM341571
Sample_status	Public on Mar 30 2009
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Mar 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	One day prior total RNA extraction, cells were grown in serum-starvation medium (DMEM supplemented with 0.5% calf serum and 400mg/ml neomycin).
Sample_growth_protocol_ch1	Stable NIH-3T3 cells were grown in DMEM supplemented with 10% calf serum and 400mg/ml neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from cell pellets using the Qiagen Rneasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 3mg total RNA using the MessageAmp II aRNA amplification kit (Ambion), according to the manufacturer's protocol.
Sample_hyb_protocol	Following fragmentation, 20mg of cRNA were hybridized for 16h at 45C on the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner System 3000.
Sample_data_processing	The array images were quantified with the GeneChip operating system (GCOS) v1.2 software (Affymetrix). The average fluorescence intensity was determined for each microarray and then the output of each experiment was globally scaled to a target value of 200. Further normalization and variance stabilization was performed using RMA in the R statistical software package.
Sample_platform_id	GPL1261
Sample_contact_name	David,,Maussang
Sample_contact_email	dmaussa@few.vu.nl
Sample_contact_laboratory	Martine J Smit/Rob Leurs
Sample_contact_department	Division of Medicinal Chemistry, Faculty of Sciences
Sample_contact_institute	Vrije Universiteit Amsterdam, Leiden/Amsterdam Center for Drug Research
Sample_contact_address	De Boelelaan 1083
Sample_contact_city	Amsterdam
Sample_contact_zip/postal_code	1081 HV
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341571/suppl/GSM341571.CEL.gz
Sample_series_id	GSE13567
Sample_data_row_count	45101

GSM341572

GPL1261

Mock (clone 13)

Stably transfected NIH-3T3 cells

Mock transfected

Sample_geo_accession	GSM341572
Sample_status	Public on Mar 30 2009
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Mar 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	One day prior total RNA extraction, cells were grown in serum-starvation medium (DMEM supplemented with 0.5% calf serum and 400mg/ml neomycin).
Sample_growth_protocol_ch1	Stable NIH-3T3 cells were grown in DMEM supplemented with 10% calf serum and 400mg/ml neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from cell pellets using the Qiagen Rneasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 3mg total RNA using the MessageAmp II aRNA amplification kit (Ambion), according to the manufacturer's protocol.
Sample_hyb_protocol	Following fragmentation, 20mg of cRNA were hybridized for 16h at 45C on the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner System 3000.
Sample_data_processing	The array images were quantified with the GeneChip operating system (GCOS) v1.2 software (Affymetrix). The average fluorescence intensity was determined for each microarray and then the output of each experiment was globally scaled to a target value of 200. Further normalization and variance stabilization was performed using RMA in the R statistical software package.
Sample_platform_id	GPL1261
Sample_contact_name	David,,Maussang
Sample_contact_email	dmaussa@few.vu.nl
Sample_contact_laboratory	Martine J Smit/Rob Leurs
Sample_contact_department	Division of Medicinal Chemistry, Faculty of Sciences
Sample_contact_institute	Vrije Universiteit Amsterdam, Leiden/Amsterdam Center for Drug Research
Sample_contact_address	De Boelelaan 1083
Sample_contact_city	Amsterdam
Sample_contact_zip/postal_code	1081 HV
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341572/suppl/GSM341572.CEL.gz
Sample_series_id	GSE13567
Sample_data_row_count	45101

GSM341573

GPL1261

US28-WT (clone 9)

Stably transfected NIH-3T3 cells

US28-WT transfected

Sample_geo_accession	GSM341573
Sample_status	Public on Mar 30 2009
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Mar 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	One day prior total RNA extraction, cells were grown in serum-starvation medium (DMEM supplemented with 0.5% calf serum and 400mg/ml neomycin).
Sample_growth_protocol_ch1	Stable NIH-3T3 cells were grown in DMEM supplemented with 10% calf serum and 400mg/ml neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from cell pellets using the Qiagen Rneasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 3mg total RNA using the MessageAmp II aRNA amplification kit (Ambion), according to the manufacturer's protocol.
Sample_hyb_protocol	Following fragmentation, 20mg of cRNA were hybridized for 16h at 45C on the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner System 3000.
Sample_data_processing	The array images were quantified with the GeneChip operating system (GCOS) v1.2 software (Affymetrix). The average fluorescence intensity was determined for each microarray and then the output of each experiment was globally scaled to a target value of 200. Further normalization and variance stabilization was performed using RMA in the R statistical software package.
Sample_platform_id	GPL1261
Sample_contact_name	David,,Maussang
Sample_contact_email	dmaussa@few.vu.nl
Sample_contact_laboratory	Martine J Smit/Rob Leurs
Sample_contact_department	Division of Medicinal Chemistry, Faculty of Sciences
Sample_contact_institute	Vrije Universiteit Amsterdam, Leiden/Amsterdam Center for Drug Research
Sample_contact_address	De Boelelaan 1083
Sample_contact_city	Amsterdam
Sample_contact_zip/postal_code	1081 HV
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341573/suppl/GSM341573.CEL.gz
Sample_series_id	GSE13567
Sample_data_row_count	45101

GSM341574

GPL1261

US28-WT (clone 23)

Stably transfected NIH-3T3 cells

US28-WT transfected

Sample_geo_accession	GSM341574
Sample_status	Public on Mar 30 2009
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Mar 30 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	One day prior total RNA extraction, cells were grown in serum-starvation medium (DMEM supplemented with 0.5% calf serum and 400mg/ml neomycin).
Sample_growth_protocol_ch1	Stable NIH-3T3 cells were grown in DMEM supplemented with 10% calf serum and 400mg/ml neomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from cell pellets using the Qiagen Rneasy Mini kit according to the manufacturer's instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 3mg total RNA using the MessageAmp II aRNA amplification kit (Ambion), according to the manufacturer's protocol.
Sample_hyb_protocol	Following fragmentation, 20mg of cRNA were hybridized for 16h at 45C on the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner System 3000.
Sample_data_processing	The array images were quantified with the GeneChip operating system (GCOS) v1.2 software (Affymetrix). The average fluorescence intensity was determined for each microarray and then the output of each experiment was globally scaled to a target value of 200. Further normalization and variance stabilization was performed using RMA in the R statistical software package.
Sample_platform_id	GPL1261
Sample_contact_name	David,,Maussang
Sample_contact_email	dmaussa@few.vu.nl
Sample_contact_laboratory	Martine J Smit/Rob Leurs
Sample_contact_department	Division of Medicinal Chemistry, Faculty of Sciences
Sample_contact_institute	Vrije Universiteit Amsterdam, Leiden/Amsterdam Center for Drug Research
Sample_contact_address	De Boelelaan 1083
Sample_contact_city	Amsterdam
Sample_contact_zip/postal_code	1081 HV
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341574/suppl/GSM341574.CEL.gz
Sample_series_id	GSE13567
Sample_data_row_count	45101

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