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Search results for the GEO ID: GSE13568

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM341583

GPL570

SW480 cells, biological rep1

colorectal cancer cell line

parent cell relatively low metastatic abilities to liver

Gene expression data

Sample_geo_accession	GSM341583
Sample_status	Public on Oct 31 2011
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Oct 31 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	A subpopulation named as M5 with enhanced metastatic abilities to liver was isolated by in vivo selection of SW480 cells
Sample_growth_protocol_ch1	cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin/streptomycin. All cell lines were maintained in humidified chamber with 5% CO2 at 37°C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with RNeasy Mini Kit (Qiagen, Chatswort, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5–10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, The biotinylated cRNA were fragmented at 94℃ for 35 min and hybridized to human Genome U133 Plus 2.0 gene chip array (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using a GeneChip Scanner 3000.
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling procedure was performed to normalize the different arrays using dChip software.
Sample_platform_id	GPL570
Sample_contact_name	Jianming,,Li
Sample_contact_institute	Southern Medical University
Sample_contact_address	Guangzhou North Road
Sample_contact_city	Guangzhou
Sample_contact_zip/postal_code	510515
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341583/suppl/GSM341583.CEL.gz
Sample_series_id	GSE13568
Sample_data_row_count	54675

GSM341584

GPL570

SW480 cells, biological rep2

colorectal cancer cell line

parent cell relatively low metastatic abilities to liver

Gene expression data

Sample_geo_accession	GSM341584
Sample_status	Public on Oct 31 2011
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Oct 31 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	A subpopulation named as M5 with enhanced metastatic abilities to liver was isolated by in vivo selection of SW480 cells
Sample_growth_protocol_ch1	cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin/streptomycin. All cell lines were maintained in humidified chamber with 5% CO2 at 37°C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with RNeasy Mini Kit (Qiagen, Chatswort, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5–10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, The biotinylated cRNA were fragmented at 94℃ for 35 min and hybridized to human Genome U133 Plus 2.0 gene chip array (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using a GeneChip Scanner 3000.
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling procedure was performed to normalize the different arrays using dChip software.
Sample_platform_id	GPL570
Sample_contact_name	Jianming,,Li
Sample_contact_institute	Southern Medical University
Sample_contact_address	Guangzhou North Road
Sample_contact_city	Guangzhou
Sample_contact_zip/postal_code	510515
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341584/suppl/GSM341584.CEL.gz
Sample_series_id	GSE13568
Sample_data_row_count	54675

GSM341585

GPL570

SW480 cells, biological rep3

colorectal cancer cell line

parent cell relatively low metastatic abilities to liver

Gene expression data

Sample_geo_accession	GSM341585
Sample_status	Public on Oct 31 2011
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Oct 31 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	A subpopulation named as M5 with enhanced metastatic abilities to liver was isolated by in vivo selection of SW480 cells
Sample_growth_protocol_ch1	cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin/streptomycin. All cell lines were maintained in humidified chamber with 5% CO2 at 37°C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with RNeasy Mini Kit (Qiagen, Chatswort, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5–10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, The biotinylated cRNA were fragmented at 94℃ for 35 min and hybridized to human Genome U133 Plus 2.0 gene chip array (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using a GeneChip Scanner 3000.
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling procedure was performed to normalize the different arrays using dChip software.
Sample_platform_id	GPL570
Sample_contact_name	Jianming,,Li
Sample_contact_institute	Southern Medical University
Sample_contact_address	Guangzhou North Road
Sample_contact_city	Guangzhou
Sample_contact_zip/postal_code	510515
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341585/suppl/GSM341585.CEL.gz
Sample_series_id	GSE13568
Sample_data_row_count	54675

GSM341586

GPL570

M5 cells, biological rep1

colorectal cancer cell line

A subpopulation named as M5 with enhanced metastatic abilities to liver enhanced metastatic abilities to liver

Gene expression data

Sample_geo_accession	GSM341586
Sample_status	Public on Oct 31 2011
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Oct 31 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	A subpopulation named as M5 with enhanced metastatic abilities to liver was isolated by in vivo selection of SW480 cells
Sample_growth_protocol_ch1	cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin/streptomycin. All cell lines were maintained in humidified chamber with 5% CO2 at 37°C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with RNeasy Mini Kit (Qiagen, Chatswort, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5–10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, The biotinylated cRNA were fragmented at 94℃ for 35 min and hybridized to human Genome U133 Plus 2.0 gene chip array (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using a GeneChip Scanner 3000.
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling procedure was performed to normalize the different arrays using dChip software.
Sample_platform_id	GPL570
Sample_contact_name	Jianming,,Li
Sample_contact_institute	Southern Medical University
Sample_contact_address	Guangzhou North Road
Sample_contact_city	Guangzhou
Sample_contact_zip/postal_code	510515
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341586/suppl/GSM341586.CEL.gz
Sample_series_id	GSE13568
Sample_data_row_count	54675

GSM341587

GPL570

M5 cells, biological rep2

colorectal cancer cell line

A subpopulation named as M5 with enhanced metastatic abilities to liver enhanced metastatic abilities to liver

Gene expression data

Sample_geo_accession	GSM341587
Sample_status	Public on Oct 31 2011
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Oct 31 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	A subpopulation named as M5 with enhanced metastatic abilities to liver was isolated by in vivo selection of SW480 cells
Sample_growth_protocol_ch1	cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin/streptomycin. All cell lines were maintained in humidified chamber with 5% CO2 at 37°C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with RNeasy Mini Kit (Qiagen, Chatswort, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5–10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, The biotinylated cRNA were fragmented at 94℃ for 35 min and hybridized to human Genome U133 Plus 2.0 gene chip array (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using a GeneChip Scanner 3000.
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling procedure was performed to normalize the different arrays using dChip software.
Sample_platform_id	GPL570
Sample_contact_name	Jianming,,Li
Sample_contact_institute	Southern Medical University
Sample_contact_address	Guangzhou North Road
Sample_contact_city	Guangzhou
Sample_contact_zip/postal_code	510515
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341587/suppl/GSM341587.CEL.gz
Sample_series_id	GSE13568
Sample_data_row_count	54675

GSM341588

GPL570

M5 cells, biological rep3

colorectal cancer cell line

A subpopulation named as M5 with enhanced metastatic abilities to liver enhanced metastatic abilities to liver

Gene expression data

Sample_geo_accession	GSM341588
Sample_status	Public on Oct 31 2011
Sample_submission_date	Nov 12 2008
Sample_last_update_date	Oct 31 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	A subpopulation named as M5 with enhanced metastatic abilities to liver was isolated by in vivo selection of SW480 cells
Sample_growth_protocol_ch1	cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin/streptomycin. All cell lines were maintained in humidified chamber with 5% CO2 at 37°C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated with RNeasy Mini Kit (Qiagen, Chatswort, CA)
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5–10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, The biotinylated cRNA were fragmented at 94℃ for 35 min and hybridized to human Genome U133 Plus 2.0 gene chip array (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using a GeneChip Scanner 3000.
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling procedure was performed to normalize the different arrays using dChip software.
Sample_platform_id	GPL570
Sample_contact_name	Jianming,,Li
Sample_contact_institute	Southern Medical University
Sample_contact_address	Guangzhou North Road
Sample_contact_city	Guangzhou
Sample_contact_zip/postal_code	510515
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341588/suppl/GSM341588.CEL.gz
Sample_series_id	GSE13568
Sample_data_row_count	54675

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