Search results for the GEO ID: GSE13569  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM341590 | GPL96 |
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AFSC at 48hrs without ethanol exposure
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Human AFSC after 48hours of parafilm sealed without ethanol
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Derived from clonal A1 line
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Expression analysis of human amniotic fluid-derived stem cells without ethanol.
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| Sample_geo_accession | GSM341590
| | Sample_status | Public on Sep 30 2009
| | Sample_submission_date | Nov 12 2008
| | Sample_last_update_date | Jun 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | AFSC were treated with 100mM etahnol (Sigma-Aldrich) and sealed with parafilm with media being replaced every 24 hours.
| | Sample_growth_protocol_ch1 | AFSC were grown in alpha-MEM medium containing 15% ES-FBS, 1% glutamine and penicillin/streptomycin supplemented with 18% Chang B and 2% Chang C (Irvine Scientific).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted byRNA Bee kit and was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr.
| | Sample_scan_protocol | Once the probe array has been hybridized, washed, and stained, it is scanned.
| | Sample_data_processing | The data were analyzed with Robust Multi-Chip Analysis (RMA).
| | Sample_platform_id | GPL96
| | Sample_contact_name | Jennifer,A,Hipp
| | Sample_contact_email | jenhipp@wfubmc.edu
| | Sample_contact_laboratory | Atala
| | Sample_contact_department | Institute for Regenerative Medicine
| | Sample_contact_institute | Wake Forest School of Medicine
| | Sample_contact_address | Medical Center Blvd
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341590/suppl/GSM341590.CEL.gz
| | Sample_series_id | GSE13569
| | Sample_data_row_count | 22283
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GSM341591 | GPL96 |
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AFSC at 48hrs with 100mM ethanol exposure
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Human AFSC after 48hours of parafilm sealed with 100mM ethanol
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Derived from clonal A1 line
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Expression analysis of human amniotic fluid-derived stem cells treated with 100mM ethanol.
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| Sample_geo_accession | GSM341591
| | Sample_status | Public on Sep 30 2009
| | Sample_submission_date | Nov 12 2008
| | Sample_last_update_date | Jun 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | AFSC were treated with 100mM etahnol (Sigma-Aldrich) and sealed with parafilm with media being replaced every 24 hours.
| | Sample_growth_protocol_ch1 | AFSC were grown in alpha-MEM medium containing 15% ES-FBS, 1% glutamine and penicillin/streptomycin supplemented with 18% Chang B and 2% Chang C (Irvine Scientific).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted byRNA Bee kit and was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr.
| | Sample_scan_protocol | Once the probe array has been hybridized, washed, and stained, it is scanned.
| | Sample_data_processing | The data were analyzed with Robust Multi-Chip Analysis (RMA).
| | Sample_platform_id | GPL96
| | Sample_contact_name | Jennifer,A,Hipp
| | Sample_contact_email | jenhipp@wfubmc.edu
| | Sample_contact_laboratory | Atala
| | Sample_contact_department | Institute for Regenerative Medicine
| | Sample_contact_institute | Wake Forest School of Medicine
| | Sample_contact_address | Medical Center Blvd
| | Sample_contact_city | Winston-Salem
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27157
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM341nnn/GSM341591/suppl/GSM341591.CEL.gz
| | Sample_series_id | GSE13569
| | Sample_data_row_count | 22283
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