Search results for the GEO ID: GSE13594  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM342095 | GPL1355 |
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CD9 transduced RASM cells, biological replicate 1.
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Rat aortic vascular smooth muscle cells transiently transduced with adenovirus containing human CD9 cDNA
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Rat vascular aortic smooth muscle cells
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Gene expression data from cells harvested after adhesion to FN for 6 hours
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| Sample_geo_accession | GSM342095
| | Sample_status | Public on Nov 12 2009
| | Sample_submission_date | Nov 13 2008
| | Sample_last_update_date | Nov 13 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | RASM cells grown to 80% confluence were infected with either Ad-CD9 or Ad-LACZ control in DMEM media. Seventy-two hours post transduction, RASM cells were harvested and replated on cell culture plates coated with 10ug/ml human plasma FN for 6 hours when adhered cells were washed and total RNA was extracted using TriZol (Sigma)
| | Sample_growth_protocol_ch1 | RASM cells were isolated from Sprague-Dawley rats and cultured in DMEM 10% FBS growth media. RASM cells were used at passage 9 in the described experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Celia,,Longhurst
| | Sample_contact_email | clonghur@utmem.edu
| | Sample_contact_phone | 901 448 3608
| | Sample_contact_fax | 901 448 7181
| | Sample_contact_laboratory | Vascular Biology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | UTHSC
| | Sample_contact_address | 956 Court Avenue Room H300
| | Sample_contact_city | Memphis
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 38163
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM342nnn/GSM342095/suppl/GSM342095.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM342nnn/GSM342095/suppl/GSM342095.CHP.gz
| | Sample_series_id | GSE13594
| | Sample_data_row_count | 31099
| | |
|
GSM342096 | GPL1355 |
|
CD9 transduced RASM cells, biological replicate 2.
|
Rat aortic vascular smooth muscle cells transiently transduced with adenovirus containing human CD9 cDNA
|
Rat vascular aortic smooth muscle cells
|
Gene expression data from cells harvested after adhesion to FN for 6 hours
|
| Sample_geo_accession | GSM342096
| | Sample_status | Public on Nov 12 2009
| | Sample_submission_date | Nov 13 2008
| | Sample_last_update_date | Nov 13 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | RASM cells grown to 80% confluence were infected with either Ad-CD9 or Ad-LACZ control in DMEM media. Seventy-two hours post transduction, RASM cells were harvested and replated on cell culture plates coated with 10ug/ml human plasma FN for 6 hours when adhered cells were washed and total RNA was extracted using TriZol (Sigma)
| | Sample_growth_protocol_ch1 | RASM cells were isolated from Sprague-Dawley rats and cultured in DMEM 10% FBS growth media. RASM cells were used at passage 9 in the described experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Celia,,Longhurst
| | Sample_contact_email | clonghur@utmem.edu
| | Sample_contact_phone | 901 448 3608
| | Sample_contact_fax | 901 448 7181
| | Sample_contact_laboratory | Vascular Biology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | UTHSC
| | Sample_contact_address | 956 Court Avenue Room H300
| | Sample_contact_city | Memphis
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 38163
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM342nnn/GSM342096/suppl/GSM342096.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM342nnn/GSM342096/suppl/GSM342096.CHP.gz
| | Sample_series_id | GSE13594
| | Sample_data_row_count | 31099
| | |
|
GSM342097 | GPL1355 |
|
Lac Z transduced RASM cells, biological replicate 1.
|
Rat aortic vascular smooth muscle cells transiently transduced with adenovirus containing Lac Z cDNA
|
Rat vascular aortic smooth muscle cells
|
Gene expression data from cells harvested after adhesion to FN for 6 hours
|
| Sample_geo_accession | GSM342097
| | Sample_status | Public on Nov 12 2009
| | Sample_submission_date | Nov 13 2008
| | Sample_last_update_date | Nov 13 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | RASM cells grown to 80% confluence were infected with either Ad-CD9 or Ad-LACZ control in DMEM media. Seventy-two hours post transduction, RASM cells were harvested and replated on cell culture plates coated with 10ug/ml human plasma FN for 6 hours when adhered cells were washed and total RNA was extracted using TriZol (Sigma)
| | Sample_growth_protocol_ch1 | RASM cells were isolated from Sprague-Dawley rats and cultured in DMEM 10% FBS growth media. RASM cells were used at passage 9 in the described experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Celia,,Longhurst
| | Sample_contact_email | clonghur@utmem.edu
| | Sample_contact_phone | 901 448 3608
| | Sample_contact_fax | 901 448 7181
| | Sample_contact_laboratory | Vascular Biology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | UTHSC
| | Sample_contact_address | 956 Court Avenue Room H300
| | Sample_contact_city | Memphis
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 38163
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM342nnn/GSM342097/suppl/GSM342097.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM342nnn/GSM342097/suppl/GSM342097.CHP.gz
| | Sample_series_id | GSE13594
| | Sample_data_row_count | 31099
| | |
|
GSM342098 | GPL1355 |
|
Lac Z transduced RASM cells, biological replicate 2.
|
Rat aortic vascular smooth muscle cells transiently transduced with adenovirus containing Lac Z cDNA
|
Rat vascular aortic smooth muscle cells
|
Gene expression data from cells harvested after adhesion to FN for 6 hours
|
| Sample_geo_accession | GSM342098
| | Sample_status | Public on Nov 12 2009
| | Sample_submission_date | Nov 13 2008
| | Sample_last_update_date | Nov 13 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | RASM cells grown to 80% confluence were infected with either Ad-CD9 or Ad-LACZ control in DMEM media. Seventy-two hours post transduction, RASM cells were harvested and replated on cell culture plates coated with 10ug/ml human plasma FN for 6 hours when adhered cells were washed and total RNA was extracted using TriZol (Sigma)
| | Sample_growth_protocol_ch1 | RASM cells were isolated from Sprague-Dawley rats and cultured in DMEM 10% FBS growth media. RASM cells were used at passage 9 in the described experiments.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Celia,,Longhurst
| | Sample_contact_email | clonghur@utmem.edu
| | Sample_contact_phone | 901 448 3608
| | Sample_contact_fax | 901 448 7181
| | Sample_contact_laboratory | Vascular Biology
| | Sample_contact_department | Medicine
| | Sample_contact_institute | UTHSC
| | Sample_contact_address | 956 Court Avenue Room H300
| | Sample_contact_city | Memphis
| | Sample_contact_state | TN
| | Sample_contact_zip/postal_code | 38163
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM342nnn/GSM342098/suppl/GSM342098.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM342nnn/GSM342098/suppl/GSM342098.CHP.gz
| | Sample_series_id | GSE13594
| | Sample_data_row_count | 31099
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