Search results for the GEO ID: GSE13639  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM343401 | GPL570 |
|
HeLa_siGFP_24_rep1
|
HeLa total RNA, 24hrs after transfection of siRNA against GFP
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after GFP knockdown for 24hrs
|
| Sample_geo_accession | GSM343401
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343401/suppl/GSM343401.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343402 | GPL570 |
|
HeLa_siDrosha_24_rep1
|
HeLa total RNA, 24hrs after transfection of siRNA against Drosha
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after Drosha knockdown for 24hrs
|
| Sample_geo_accession | GSM343402
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343402/suppl/GSM343402.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343403 | GPL570 |
|
HeLa_siGFP_48_rep1
|
HeLa total RNA, 48hrs after transfection of siRNA against GFP
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after GFP knockdown for 48hrs
|
| Sample_geo_accession | GSM343403
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343403/suppl/GSM343403.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343404 | GPL570 |
|
HeLa_siDrosha_48_rep1
|
HeLa total RNA, 48hrs after transfection of siRNA against Drosha
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after Drosha knockdown for 48hrs
|
| Sample_geo_accession | GSM343404
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343404/suppl/GSM343404.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343405 | GPL570 |
|
HeLa_siGFP_24
|
HeLa total RNA, 24hrs after transfection of siRNA against GFP
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after GFP knockdown for 24hrs
|
| Sample_geo_accession | GSM343405
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343405/suppl/GSM343405.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343406 | GPL570 |
|
HeLa_siDrosha_24_rep2
|
HeLa total RNA, 24hrs after transfection of siRNA against Drosha
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after Drosha knockdown for 24hrs
|
| Sample_geo_accession | GSM343406
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343406/suppl/GSM343406.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343407 | GPL570 |
|
HeLa_siDGCR8_24_rep1
|
HeLa total RNA, 24hrs after transfection of siRNA against DGCR8
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after DGCR8 knockdown for 24hrs
|
| Sample_geo_accession | GSM343407
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343407/suppl/GSM343407.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343408 | GPL570 |
|
HeLa_siDGCR8_24_rep2
|
HeLa total RNA, 24hrs after transfection of siRNA against DGCR8
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after DGCR8 knockdown for 24hrs
|
| Sample_geo_accession | GSM343408
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343408/suppl/GSM343408.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343409 | GPL570 |
|
HeLa_siGFP_48
|
HeLa total RNA, 48hrs after transfection of siRNA against GFP
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after GFP knockdown for 48hrs
|
| Sample_geo_accession | GSM343409
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343409/suppl/GSM343409.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343410 | GPL570 |
|
HeLa_siDrosha_48_rep2
|
HeLa total RNA, 48hrs after transfection of siRNA against Drosha
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after Drosha knockdown for 48hrs
|
| Sample_geo_accession | GSM343410
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343410/suppl/GSM343410.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343411 | GPL570 |
|
HeLa_siDGCR8_48_rep1
|
HeLa total RNA, 48hrs after transfection of siRNA against DGCR8
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after DGCR8 knockdown for 48hrs
|
| Sample_geo_accession | GSM343411
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343411/suppl/GSM343411.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
| | |
|
GSM343412 | GPL570 |
|
HeLa_siDGCR8_48_rep2
|
HeLa total RNA, 48hrs after transfection of siRNA against DGCR8
|
Cervical cancer cell line
|
Expression changes of HeLa mRNA after DGCR8 knockdown for 48hrs
|
| Sample_geo_accession | GSM343412
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Nov 18 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 32nM of siRNAs (Samchully Phamaceuticals) were transfected into HeLa cells with lipofectamine 2000 (InvitroGen).
| | Sample_growth_protocol_ch1 | HeLa cells were cultured in DMEM supplemented with 10% FBS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jinju,,Han
| | Sample_contact_laboratory | Bd. 504, Rm 501
| | Sample_contact_institute | Seoul National University
| | Sample_contact_address | 599 Gwanangno, Gwanak-gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 151-742
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343412/suppl/GSM343412.CEL.gz
| | Sample_series_id | GSE13639
| | Sample_series_id | GSE13640
| | Sample_data_row_count | 54675
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