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Search results for the GEO ID: GSE1364
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GPL ID
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Title
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Description
Characteristics
GSM22051
GPL96
E1_T2_R1, Spheroid, no storage Kidney 293 HEK Cells cell type: 3-D spheroid stress: no dry storage time: n/a antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22052
GPL96
E1_T2_R2, Spheroid, no storage Kidney 293 HEK Cells cell type: 3-D spheroid stress: no dry storage time: n/a antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22053
GPL96
E1_T2_R3, Spheroid, no storage Kidney 293 HEK Cells cell type: 3-D spheroid stress: no dry storage time: n/a antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22054
GPL96
E1_T3_R1, two week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 2 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22055
GPL96
E1_T3_R2, two week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 2 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22056
GPL96
E1_T3_R3, two week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 2 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22057
GPL96
E1_T4_R1, two week storage/full recovery Kidney 293 HEK Cells cell type: 2-D monolayer stress: 2 wk dry storage time: 72 hr recovery antibiotics (yes or no?): yes 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22058
GPL96
E1_T4_R2, two week storage/full recovery Kidney 293 HEK Cells cell type: 2-D monolayer stress: 2 wk dry storage time: 72 hr recovery antibiotics (yes or no?): yes 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22059
GPL96
E1_T4_R3, two week storage/full recovery Kidney 293 HEK Cells cell type: 2-D monolayer stress: 2 wk dry storage time: 72 hr recovery antibiotics (yes or no?): yes 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22060
GPL96
E1_T5_R1, four week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 4 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22061
GPL96
E1_T5_R2, four week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 4 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22062
GPL96
E1_T5_R3, four week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 4 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22063
GPL96
E1_T6_R1, six week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 6 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22064
GPL96
E1_T6_R2, six week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 6 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22065
GPL96
E1_T6_R3, six week storage/0hr recovery Kidney 293 HEK Cells cell type: 3-D spheroid stress: 6 wk dry storage time: 0 h recovery antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22066
GPL96
E1_T7_R1, Control, monolayer, antibiotics Kidney 293 HEK Cells cell type: 2-D monolayer stress: no dry storage time: n/a antibiotics (yes or no?): yes 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22067
GPL96
E1_T7_R2, Control, monolayer, antibiotics Kidney 293 HEK Cells cell type: 2-D monolayer stress: no dry storage time: n/a antibiotics (yes or no?): yes 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22068
GPL96
E1_T7_R3, Control, monolayer, antibiotics Kidney 293 HEK Cells cell type: 2-D monolayer stress: no dry storage time: n/a antibiotics (yes or no?): yes 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22069
GPL96
E1_T1_R1, Control, monolayer Kidney 293 HEK Cells cell type: 2-D monolayer stress: no dry storage time: n/a antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22070
GPL96
E1_T1_R2, Control, monolayer Kidney 293 HEK Cells cell type: 2-D monolayer stress: no dry storage time: n/a antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
GSM22071
GPL96
E1_T1_R3, Control, monolayer Kidney 293 HEK Cells cell type: 2-D monolayer stress: no dry storage time: n/a antibiotics (yes or no?): no 1x10^7 Human embryonic kidney cell spheroids were grown for 7 days in suspension over agarose prepared with FBS supplemented DMEM containing 50mM HEPES, pH 7.2, and 1xGlutamax-1 (Invitrogen). Following growth, media was removed and the spheroids were stored on the surface of the agarose in sealed flasks for 2,4, and 6 week intervals at room temperature in the dark. The samples were compared against unstored controls of both spheroids and monolayer (at 30-40% confluence)samples. All samples were conducted in triplicate. For each storage timepoint, a set of unrehydrated samples were analyzed, and an additional set of rehydrated samples were analyzed after allowing for growth in a treated tissue culture flask, to assess how well the samples returned to a normal, pre-storage state. Rehydration was conducted with DMEM (Invitrogen) containing 20%FBS and antibiotics (100U penicillin, 100ug/mL streptomycin, and 0.25ug Fungizone), with HEPES and Glutamax as stated previously.
 
 
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