Search results for the GEO ID: GSE13669  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM343768 | GPL1355 |
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Rat_Kidney_Medullary_Thick_Ascending_Limb_1
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Medullary thick ascending limb of Henle’s of rat
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Strain: Sprague-Dawley
Sex: Male
Age: 7-8 weeks old
Tissue: Medullary thick ascending limb of Henle’s
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Profiling of gene expression in mTAL cell of rat
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| Sample_geo_accession | GSM343768
| | Sample_status | Public on Jan 26 2009
| | Sample_submission_date | Nov 19 2008
| | Sample_last_update_date | Jan 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were injected with 250 u of heparin and 1 mg of furosemide intraperitoneum 30 minutes before decapitation. Retrograde perfusion through abdominal aorta with 10 ml of ice cold PBS and 10 ml of pre-warmed collagenase solution (1 mg/ml collagenase, 0.5 mg/ml hyarulonidase). Outer medulla were excised and digested in collagenase solution for 60 minutes with 95% O2 and 5% CO2. mTAL were separate from other tissue by gradient centrifugation in 33.3% Percoll solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted by using Trizol reagent according to manufacturer's protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
| | Sample_hyb_protocol | Labeled cRNA were hybridized to Rat 230 2.0 Array in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
| | Sample_data_processing | Affymetrix default parameters, Algorithm: ExpressionStat 5.0
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ming-Jiun,,Yu
| | Sample_contact_email | mjyu@ntu.edu.tw
| | Sample_contact_laboratory | Systems Biology of Epithelia Laboratory
| | Sample_contact_department | Institute of Biochemistry and Molecular Biology
| | Sample_contact_institute | National Taiwan University College of Medicine
| | Sample_contact_address | Rm 816, No. 1 Ren-Ai Road Section 1
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 100
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | http://sbel.mc.ntu.edu.tw
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343768/suppl/GSM343768.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343768/suppl/GSM343768.CHP.gz
| | Sample_series_id | GSE13669
| | Sample_series_id | GSE13672
| | Sample_data_row_count | 31099
| | |
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GSM343769 | GPL1355 |
|
Rat_Kidney_Medullary_Thick_Ascending_Limb_2
|
Medullary thick ascending limb of Henle of rat
|
Strain: Sprague-Dawley
Sex: Male
Age: 7-8 weeks old
Tissue: Medullary thick ascending limb of Henle’s
|
Profiling of gene expression in mTAL cell of rat
|
| Sample_geo_accession | GSM343769
| | Sample_status | Public on Jan 26 2009
| | Sample_submission_date | Nov 19 2008
| | Sample_last_update_date | Jan 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were injected with 250 u of heparin and 1 mg of furosemide intraperitoneum 30 minutes before decapitation. Retrograde perfusion through abdominal aorta with 10 ml of ice cold PBS and 10 ml of pre-warmed collagenase solution (1 mg/ml collagenase, 0.5 mg/ml hyarulonidase). Outer medulla were excised and digested in collagenase solution for 60 minutes with 95% O2 and 5% CO2. mTAL were separate from other tissue by gradient centrifugation in 33.3% Percoll solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted by using Trizol reagent according to manufacturer's protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
| | Sample_hyb_protocol | Labeled cRNA were hybridized to Rat 230 2.0 Array in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
| | Sample_data_processing | Affymetrix default parameters, Algorithm: ExpressionStat 5.0
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ming-Jiun,,Yu
| | Sample_contact_email | mjyu@ntu.edu.tw
| | Sample_contact_laboratory | Systems Biology of Epithelia Laboratory
| | Sample_contact_department | Institute of Biochemistry and Molecular Biology
| | Sample_contact_institute | National Taiwan University College of Medicine
| | Sample_contact_address | Rm 816, No. 1 Ren-Ai Road Section 1
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 100
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | http://sbel.mc.ntu.edu.tw
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343769/suppl/GSM343769.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343769/suppl/GSM343769.CHP.gz
| | Sample_series_id | GSE13669
| | Sample_series_id | GSE13672
| | Sample_data_row_count | 31099
| | |
|
GSM343770 | GPL1355 |
|
Rat_Kidney_Medullary_Thick_Ascending_Limb_3
|
Medullary thick ascending limb of Henle of rat
|
Strain: Sprague-Dawley
Sex: Male
Age: 7-8 weeks old
Tissue: Medullary thick ascending limb of Henle’s
|
Profiling of gene expression in mTAL cell of rat
|
| Sample_geo_accession | GSM343770
| | Sample_status | Public on Jan 26 2009
| | Sample_submission_date | Nov 19 2008
| | Sample_last_update_date | Jan 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were injected with 250 u of heparin and 1 mg of furosemide intraperitoneum 30 minutes before decapitation. Retrograde perfusion through abdominal aorta with 10 ml of ice cold PBS and 10 ml of pre-warmed collagenase solution (1 mg/ml collagenase, 0.5 mg/ml hyarulonidase). Outer medulla were excised and digested in collagenase solution for 60 minutes with 95% O2 and 5% CO2. mTAL were separate from other tissue by gradient centrifugation in 33.3% Percoll solution.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA were extracted by using Trizol reagent according to manufacturer's protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
| | Sample_hyb_protocol | Labeled cRNA were hybridized to Rat 230 2.0 Array in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
| | Sample_data_processing | Affymetrix default parameters, Algorithm: ExpressionStat 5.0
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ming-Jiun,,Yu
| | Sample_contact_email | mjyu@ntu.edu.tw
| | Sample_contact_laboratory | Systems Biology of Epithelia Laboratory
| | Sample_contact_department | Institute of Biochemistry and Molecular Biology
| | Sample_contact_institute | National Taiwan University College of Medicine
| | Sample_contact_address | Rm 816, No. 1 Ren-Ai Road Section 1
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 100
| | Sample_contact_country | Taiwan
| | Sample_contact_web_link | http://sbel.mc.ntu.edu.tw
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343770/suppl/GSM343770.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM343nnn/GSM343770/suppl/GSM343770.CHP.gz
| | Sample_series_id | GSE13669
| | Sample_series_id | GSE13672
| | Sample_data_row_count | 31099
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