Search results for the GEO ID: GSE13712  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM344778 | GPL570 |
|
Vein endothelial cells_Young_Static_rep1
|
Human umbilical vein endothelial cells, young, static
|
24hr
young (p4)
|
Replicate 1 of 3
|
| Sample_geo_accession | GSM344778
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344778/suppl/GSM344778.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344779 | GPL570 |
|
Vein endothelial cells_Young_Static_rep2
|
Human umbilical vein endothelial cells, young, static
|
24hr
young (p4)
|
Replicate 2 of 3
|
| Sample_geo_accession | GSM344779
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344779/suppl/GSM344779.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344780 | GPL570 |
|
Vein endothelial cells_Young_Static_rep3
|
Human umbilical vein endothelial cells, young, static
|
24hr
young (p4)
|
Replicate 3 of 3
|
| Sample_geo_accession | GSM344780
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344780/suppl/GSM344780.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344781 | GPL570 |
|
Vein endothelial cells_Young_Shear_rep1
|
Human umbilical vein endothelial cells, young, shear
|
24hr
young (p4)
|
Replicate 1 of 3
|
| Sample_geo_accession | GSM344781
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344781/suppl/GSM344781.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344782 | GPL570 |
|
Vein endothelial cells_Young_Shear_rep2
|
Human umbilical vein endothelial cells, young, shear
|
24hr
young (p4)
|
Replicate 2 of 3
|
| Sample_geo_accession | GSM344782
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344782/suppl/GSM344782.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344783 | GPL570 |
|
Vein endothelial cells_Young_Shear_rep3
|
Human umbilical vein endothelial cells, young, shear
|
24hr
young (p4)
|
Replicate 3 of 3
|
| Sample_geo_accession | GSM344783
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344783/suppl/GSM344783.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344784 | GPL570 |
|
Vein endothelial cells_Old_Static_rep1
|
Human umbilical vein endothelial cells, old, static
|
24hr
senescent (p18)
|
Replicate 1 of 3
|
| Sample_geo_accession | GSM344784
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344784/suppl/GSM344784.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344785 | GPL570 |
|
Vein endothelial cells_Old_Static_rep2
|
Human umbilical vein endothelial cells, old, static
|
24hr
senescent (p18)
|
Replicate 2 of 3
|
| Sample_geo_accession | GSM344785
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344785/suppl/GSM344785.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344786 | GPL570 |
|
Vein endothelial cells_Old_Static_rep3
|
Human umbilical vein endothelial cells, old, static
|
24hr
senescent (p18)
|
Replicate 3 of 3
|
| Sample_geo_accession | GSM344786
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344786/suppl/GSM344786.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344787 | GPL570 |
|
Vein endothelial cells_Old_Shear_rep1
|
Human umbilical vein endothelial cells, old, shear
|
24hr
senescent (p18)
|
Replicate 1 of 3
|
| Sample_geo_accession | GSM344787
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344787/suppl/GSM344787.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344788 | GPL570 |
|
Vein endothelial cells_Old_Shear_rep2
|
Human umbilical vein endothelial cells, old, shear
|
24hr
senescent (p18)
|
Replicate 2 of 3
|
| Sample_geo_accession | GSM344788
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344788/suppl/GSM344788.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
|
GSM344789 | GPL570 |
|
Vein endothelial cells_Old_Shear_rep3
|
Human umbilical vein endothelial cells, old, shear
|
24hr
senescent (p18)
|
Replicate 3 of 3
|
| Sample_geo_accession | GSM344789
| | Sample_status | Public on May 23 2009
| | Sample_submission_date | Nov 23 2008
| | Sample_last_update_date | May 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were serially passaged until they showed phenotypes of senescence. Young (p4) and senescent (p18) cells grown on 100 mm culture dishes were exposed to laminar shear stress at 12 dyn•cm-2 for 24 hours. Laminar shear stress was provided by rotating a Teflon cone (0.5° cone angle) mounted onto a culture dish.
| | Sample_growth_protocol_ch1 | HUVECs purchased from Clonetics Cambrex (Rockland, ME, USA) were cultured on 0.2% gelatin-coated 100-mm tissue culture dishes (BD Biosciences, San Jose, CA, USA) at 37 °C and 5% CO2 in endothelial cell growth medium EBM-2 (Clonetics Cambrex) with endothelial growth supplements and 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction with Qiagen RNeasy mini kit was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The MAS5 algorithm was used for expression summary and signal calculation of Affymetrix Human Genome U133 Plus 2.0 data. Global scaling normalization was performed and then the normalized data were log-transformed with base 2.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Yong Chool,,Boo
| | Sample_contact_email | ycboo@knu.ac.kr
| | Sample_contact_laboratory | Free Radical Biology
| | Sample_contact_department | Department of Molecular Medicine
| | Sample_contact_institute | Kyungpook National University School of Medicine
| | Sample_contact_address | 101 Dongindong
| | Sample_contact_city | Daegu
| | Sample_contact_zip/postal_code | 700-422
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM344nnn/GSM344789/suppl/GSM344789.CEL.gz
| | Sample_series_id | GSE13712
| | Sample_data_row_count | 54675
| | |
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