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Search results for the GEO ID: GSE13720
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GSM256733
GPL339
CD4-CD8-_DN_fetal_thymocytes_TCDD CD4-CD8-_DN_fetal_thymocytes Strain: C57BL/6 Tissue: Thymus Cell Type: CD4-CD8- Thymocytes Age: dpc 21 Treatment: 10 nM 2,3,7,8-Tetrachlorodibenzo-p-dioxin Replicate: 1 C57BL/6 dams were terminally mated and fetuses surgically removed at dpc 15. Fetal thymus organ culture were performed as described before. Briefly 5-6 fetal thymus lobes were cultured on culture inlays for 6 days. Medium (RPMI 1640, with 10nM TCDD or solvent control) was excanged every 2 days. At day six thymus lobes were washed 2x with ice cold PBS and transfered to single cell suspensions by gently grinding. CD4-CD8- were subsequently sorted with anti CD4 and anti CD8 MACS beads to a purity >95% which was routinely checked by FACS analysis.
GSM256734
GPL339
CD4-CD8-_DN_fetal_thymocytes_Dioxane CD4-CD8-_DN_fetal_thymocytes Strain: C57BL/6 Tissue: Thymus Cell Type: CD4-CD8- Thymocytes Age: dpc 21 Treatment: 0.1%V Dioxane Replicate: 1 C57BL/6 dams were terminally mated and fetuses surgically removed at dpc 15. Fetal thymus organ culture were performed as described before. Briefly 5-6 fetal thymus lobes were cultured on culture inlays for 6 days. Medium (RPMI 1640, with 10nM TCDD or solvent control) was excanged every 2 days. At day six thymus lobes were washed 2x with ice cold PBS and transfered to single cell suspensions by gently grinding. CD4-CD8- were subsequently sorted with anti CD4 and anti CD8 MACS beads to a purity >95% which was routinely checked by FACS analysis.
GSM256735
GPL339
CD4-CD8-_DN_fetal_thymus_emigrants_Dioxane CD4-CD8-_DN_fetal_thymus emigrants Strain: C57BL/6 Tissue: Thymusemigrants Cell Type: CD4-CD8- Thymocytes Age: dpc 21 Treatment: Dioxane Replicate: 1 C57BL/6 dams were terminally mated and fetuses surgically removed at dpc 15. Fetal thymus organ culture were performed as described before. Briefly 5-6 fetal thymus lobes were cultured on culture inlays for 6 days. Medium (RPMI 1640, with 10nM TCDD or solvent control) was excanged every 2 days. At day six thymus lobes were flushed 2x with 200µl ice cold PBS, which was recovered for cell isolation. Thymus emigrants were transfered to single cell suspensions by gently grinding. CD4-CD8- were subsequently sorted with anti CD4 and anti CD8 MACS beads to a purity >95% which was routinely checked by FACS analysis.
GSM256736
GPL339
CD4-CD8-_DN_fetal_thymus_emigrants_Dioxane_2 CD4-CD8-_DN_fetal_thymus emigrants Strain: C57BL/6 Tissue: Thymusemigrants Cell Type: CD4-CD8- Thymocytes Age: dpc 21 Treatment: Dioxane 2 Replicate: 2 C57BL/6 dams were terminally mated and fetuses surgically removed at dpc 15. Fetal thymus organ culture were performed as described before. Briefly 5-6 fetal thymus lobes were cultured on culture inlays for 6 days. Medium (RPMI 1640, with 10nM TCDD or solvent control) was excanged every 2 days. At day six thymus lobes were flushed 2x with 200µl ice cold PBS, which was recovered for cell isolation. Thymus emigrants were transfered to single cell suspensions by gently grinding. CD4-CD8- were subsequently sorted with anti CD4 and anti CD8 MACS beads to a purity >95% which was routinely checked by FACS analysis.
GSM256737
GPL339
CD4-CD8-_DN_fetal_thymus_emigrants_TCDD_1 CD4-CD8-_DN_fetal_thymus emigrants Strain: C57BL/6 Tissue: Thymusemigrants Cell Type: CD4-CD8- Thymocytes Age: dpc 21 Treatment: 10 nM 2,3,7,8-Tetrachlorodibenzo-p-dioxin Replicate: 1 C57BL/6 dams were terminally mated and fetuses surgically removed at dpc 15. Fetal thymus organ culture were performed as described before. Briefly 5-6 fetal thymus lobes were cultured on culture inlays for 6 days. Medium (RPMI 1640, with 10nM TCDD or solvent control) was excanged every 2 days. At day six thymus lobes were flushed 2x with 200µl ice cold PBS, which was recovered for cell isolation. Thymus emigrants were transfered to single cell suspensions by gently grinding. CD4-CD8- were subsequently sorted with anti CD4 and anti CD8 MACS beads to a purity >95% which was routinely checked by FACS analysis.
GSM256738
GPL339
CD4-CD8-_DN_fetal_thymus_emigrants_TCDD_2 CD4-CD8-_DN_fetal_thymus emigrants Strain: C57BL/6 Tissue: Thymusemigrants Cell Type: CD4-CD8- Thymocytes Age: dpc 21 Treatment: 10 nM 2,3,7,8-Tetrachlorodibenzo-p-dioxin Replicate: 2 C57BL/6 dams were terminally mated and fetuses surgically removed at dpc 15. Fetal thymus organ culture were performed as described before. Briefly 5-6 fetal thymus lobes were cultured on culture inlays for 6 days. Medium (RPMI 1640, with 10nM TCDD or solvent control) was excanged every 2 days. At day six thymus lobes were flushed 2x with 200µl ice cold PBS, which was recovered for cell isolation. Thymus emigrants were transfered to single cell suspensions by gently grinding. CD4-CD8- were subsequently sorted with anti CD4 and anti CD8 MACS beads to a purity >95% which was routinely checked by FACS analysis.
 
 
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