Search results for the GEO ID: GSE13747 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM345979 | GPL1355 |
|
Liver_Bile_Duct_Ligation_1
|
Control liver
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM345979
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Abdominal incision as a sham operation at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM345nnn/GSM345979/suppl/GSM345979.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346050 | GPL1355 |
|
Liver_Bile_Duct_Ligation_2
|
Control liver
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346050
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Abdominal incision as a sham operation at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346050/suppl/GSM346050.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346052 | GPL1355 |
|
Liver_Bile_Duct_Ligation_3
|
Control liver
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346052
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | aline infused by sub-cutaneous osmotic mini-pump. Abdominal incision as a sham operation at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346052/suppl/GSM346052.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346053 | GPL1355 |
|
Liver_Bile_Duct_Ligation_4
|
Control liver
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346053
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Abdominal incision as a sham operation at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346053/suppl/GSM346053.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346054 | GPL1355 |
|
Liver_Bile_Duct_Ligation_5
|
Control liver
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346054
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Abdominal incision as a sham operation at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346054/suppl/GSM346054.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346055 | GPL1355 |
|
Liver_Bile_Duct_Ligation_6
|
Control liver
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346055
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Abdominal incision as a sham operation at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346055/suppl/GSM346055.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346056 | GPL1355 |
|
Liver_Bile_Duct_Ligation_7
|
Liver from rats submitted to Bile Duct Ligation for two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346056
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346056/suppl/GSM346056.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346057 | GPL1355 |
|
Liver_Bile_Duct_Ligation_8
|
Liver from rats submitted to Bile Duct Ligation for two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346057
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346057/suppl/GSM346057.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346058 | GPL1355 |
|
Liver_Bile_Duct_Ligation_9
|
Liver from rats submitted to Bile Duct Ligation for two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346058
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346058/suppl/GSM346058.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346059 | GPL1355 |
|
Liver_Bile_Duct_Ligation_10
|
Liver from rats submitted to Bile Duct Ligation for two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346059
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346059/suppl/GSM346059.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346060 | GPL1355 |
|
Liver_Bile_Duct_Ligation_11
|
Liver from rats submitted to Bile Duct Ligation for two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346060
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346060/suppl/GSM346060.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346061 | GPL1355 |
|
Liver_Bile_Duct_Ligation_12
|
Liver from rats submitted to Bile Duct Ligation for two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346061
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Saline infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346061/suppl/GSM346061.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346062 | GPL1355 |
|
Liver_Bile_Duct_Ligation_13
|
Liver from rats submitted to Bile Duct Ligation for two weeks and infused with ghrelin throughout these two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346062
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Ghrelin infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346062/suppl/GSM346062.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346063 | GPL1355 |
|
Liver_Bile_Duct_Ligation_14
|
Liver from rats submitted to Bile Duct Ligation for two weeks and infused with ghrelin throughout these two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346063
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Ghrelin infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346063/suppl/GSM346063.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346064 | GPL1355 |
|
Liver_Bile_Duct_Ligation_15
|
Liver from rats submitted to Bile Duct Ligation for two weeks and infused with ghrelin throughout these two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346064
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Ghrelin infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346064/suppl/GSM346064.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346065 | GPL1355 |
|
Liver_Bile_Duct_Ligation_16
|
Liver from rats submitted to Bile Duct Ligation for two weeks and infused with ghrelin throughout these two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346065
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Ghrelin infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346065/suppl/GSM346065.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
GSM346066 | GPL1355 |
|
Liver_Bile_Duct_Ligation_17
|
Liver from rats submitted to Bile Duct Ligation for two weeks and infused with ghrelin throughout these two weeks.
|
Liver of male wistar rats, 9 weeks.
|
RNA was isolated from rat livers using the QIAGEN RNeasy kit (Qiagen). RNA integrity was checked with the Agilent 2100 Bioanalyser (Agilent Technologies) and only high quality RNA samples were hybridized to Rat Genome 230 2.0 GeneChips (Affymetrix). Briefly, 2 µg of total RNA were used to generate double strand cDNA using an oligo dT- primer containing the T7 RNA polymerase promoter site and the SuperScript Choice System kit (Invitrogen).
|
Sample_geo_accession | GSM346066
| Sample_status | Public on Dec 03 2008
| Sample_submission_date | Nov 26 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Ghrelin infused by sub-cutaneous osmotic mini-pump. Bile Duct Ligation was performed at week 7. At week 9 rat was sacrificed.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | QIAGEN RNeasy kit
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was purified by the GeneChip Sample Clean Up Module, followed by in vitro synthesis of biotinylated cRNA using the BioArray High Yield RNA transcription kit (Affymetrix). The arrays were then washed and labelled with streptavidin-phycoerythrin (SAPE), and the signal was amplified with an anti-streptavidin biotinylated antibody followed by a second round of staining with SAPE using an Affymetrix fluidics station 450.
| Sample_hyb_protocol | The resulting cRNA was purified and fragmented and 15 µg were hybridized to RAT Genome 230 2.0 GeneChips for 16 hours , at 45ºC and 60 rpm
| Sample_scan_protocol | The labelled arrays were scanned with a Gene chip scanner 3000.
| Sample_data_processing | Data processing cel files were normalized with 'gcrma' function of the Bioconductor package 'gcrma' in R.
| Sample_platform_id | GPL1355
| Sample_contact_name | Ramón,,Bataller
| Sample_contact_email | bataller@clinic.ub.es
| Sample_contact_department | Liver Unit
| Sample_contact_institute | Hospital Clínic, IDIBAPS
| Sample_contact_address | C/Casanova, 143
| Sample_contact_city | Barcelona
| Sample_contact_zip/postal_code | 08036
| Sample_contact_country | Spain
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM346nnn/GSM346066/suppl/GSM346066.CEL.gz
| Sample_series_id | GSE13747
| Sample_data_row_count | 31099
| |
|
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