Search results for the GEO ID: GSE13802  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM347091 | GPL1355 |
|
proximal colon AOM rat 1
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347091
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347091/suppl/GSM347091.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347091/suppl/GSM347091.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347092 | GPL1355 |
|
proximal colon AOM rat 2
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347092
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347092/suppl/GSM347092.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347092/suppl/GSM347092.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347093 | GPL1355 |
|
proximal colon AOM rat 3
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347093
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347093/suppl/GSM347093.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347093/suppl/GSM347093.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347094 | GPL1355 |
|
proximal colon AOM rat 4
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347094
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347094/suppl/GSM347094.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347094/suppl/GSM347094.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347095 | GPL1355 |
|
proximal colon AOM rat 5
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347095
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347095/suppl/GSM347095.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347095/suppl/GSM347095.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347096 | GPL1355 |
|
proximal colon AOM rat 6
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347096
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347096/suppl/GSM347096.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347096/suppl/GSM347096.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347097 | GPL1355 |
|
proximal colon AOM rat 7
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347097
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347097/suppl/GSM347097.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347097/suppl/GSM347097.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347098 | GPL1355 |
|
proximal colon AOM rat 8
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347098
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347098/suppl/GSM347098.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347098/suppl/GSM347098.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347099 | GPL1355 |
|
proximal colon AOM rat 10
|
Rat scraped colonic epithelium (proximal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347099
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347099/suppl/GSM347099.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347099/suppl/GSM347099.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347100 | GPL1355 |
|
distal colon AOM rat 1
|
Rat scraped colonic epithelium (distal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347100
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347100/suppl/GSM347100.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347100/suppl/GSM347100.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347101 | GPL1355 |
|
distal colon AOM rat 2
|
Rat scraped colonic epithelium (distal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347101
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347101/suppl/GSM347101.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347101/suppl/GSM347101.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347102 | GPL1355 |
|
distal colon AOM rat 3
|
Rat scraped colonic epithelium (distal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347102
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347102/suppl/GSM347102.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347102/suppl/GSM347102.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347103 | GPL1355 |
|
distal colon AOM rat 4
|
Rat scraped colonic epithelium (distal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347103
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347103/suppl/GSM347103.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347103/suppl/GSM347103.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347104 | GPL1355 |
|
distal colon AOM rat 5
|
Rat scraped colonic epithelium (distal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347104
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347104/suppl/GSM347104.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347104/suppl/GSM347104.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347105 | GPL1355 |
|
distal colon AOM rat 6
|
Rat scraped colonic epithelium (distal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347105
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347105/suppl/GSM347105.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347105/suppl/GSM347105.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347106 | GPL1355 |
|
distal colon AOM rat 7
|
Rat scraped colonic epithelium (distal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347106
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347106/suppl/GSM347106.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347106/suppl/GSM347106.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
| | |
|
GSM347107 | GPL1355 |
|
distal colon AOM rat 8
|
Rat scraped colonic epithelium (distal)
|
Dawley rats weighing approximately 176 +- 2.4g
|
Gene expression data from azoxymethane treated rat colonic epithelium.
|
| Sample_geo_accession | GSM347107
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347107/suppl/GSM347107.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347107/suppl/GSM347107.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
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GSM347108 | GPL1355 |
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distal colon AOM rat 10
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Rat scraped colonic epithelium (distal)
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Dawley rats weighing approximately 176 +- 2.4g
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Gene expression data from azoxymethane treated rat colonic epithelium.
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| Sample_geo_accession | GSM347108
| | Sample_status | Public on Dec 05 2008
| | Sample_submission_date | Dec 02 2008
| | Sample_last_update_date | Dec 04 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_biomaterial_provider_ch1 | Animal Resource Centre, Western Australia
| | Sample_treatment_protocol_ch1 | Rats were injected with azoxymethane (AOM; 15 mg/kg subcutaneously). Six hours after injection the rats were anaesthetised with isoflurane and killed by exsanguination. The large bowel (excluding the rectum) was removed, opened longitudinally along the mesenteric border and digesta removed. The colon was rinsed clean with PBS and transferred to a chilled ceramic plate for dissection and the distal and proximal sections scraped off with a microscope slide and placed in RNAlater solution then stored at -80oC | | Sample_growth_protocol | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle.
| | Sample_growth_protocol_ch1 | Animals were housed in wire-bottomed caging in a temperature controlled room (22-24°C) with a 12 h light/dark cycle. They were randomly allocated into two groups (n=20) with approximately equal body weights. They were given free access to water and modified AIN-93G diet.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by QIAGEN RNAeasy column cleanup.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Approximately 4.5 µg of total RNA was processed to produce biotinylated cRNA targets according to the standard Affymetrix protocol.
| | Sample_hyb_protocol | Standard Affymetrix procedures.
| | Sample_scan_protocol | Standard Affymetrix procedures.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Caroline,Audrey,Kerr
| | Sample_contact_email | Caroline.Kerr@csiro.au
| | Sample_contact_phone | +61 2 94905063
| | Sample_contact_fax | +61 2 94905010
| | Sample_contact_laboratory | North Ryde
| | Sample_contact_department | Moelcular health Technologies
| | Sample_contact_institute | CSIRO
| | Sample_contact_address | 11 Julius Ave North Ryde
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2113
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347108/suppl/GSM347108.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347108/suppl/GSM347108.CHP.gz
| | Sample_series_id | GSE13802
| | Sample_data_row_count | 31099
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