Search results for the GEO ID: GSE13806  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM347160 | GPL570 |
|
In vitro sFRP1_1
|
cultured MDA-MB-231/sFRP1 cell
|
MDA-MB-231/sFRP1 cells
|
cultured MDA-MB-231/sFRP1 cell
|
| Sample_geo_accession | GSM347160
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347160/suppl/GSM347160.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347161 | GPL570 |
|
In vitro sFRP1_2
|
cultured MDA-MB-231/sFRP1 cell
|
MDA-MB-231/sFRP1 cells
|
cultured MDA-MB-231/sFRP1 cell
|
| Sample_geo_accession | GSM347161
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347161/suppl/GSM347161.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347162 | GPL570 |
|
In vitro sFRP1_3
|
cultured MDA-MB-231/sFRP1 cell
|
MDA-MB-231/sFRP1 cells
|
cultured MDA-MB-231/sFRP1 cell
|
| Sample_geo_accession | GSM347162
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347162/suppl/GSM347162.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347163 | GPL570 |
|
In vitro control_1
|
cultured MDA-MB-231/control cell
|
MDA-MB-231/control cells
|
cultured MDA-MB-231/control cell
|
| Sample_geo_accession | GSM347163
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347163/suppl/GSM347163.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347164 | GPL570 |
|
In vitro control_2
|
cultured MDA-MB-231/control cell
|
MDA-MB-231/control cells
|
cultured MDA-MB-231/control cell
|
| Sample_geo_accession | GSM347164
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347164/suppl/GSM347164.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347165 | GPL570 |
|
In vitro control_3
|
cultured MDA-MB-231/control cell
|
MDA-MB-231/control cells
|
cultured MDA-MB-231/control cell
|
| Sample_geo_accession | GSM347165
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347165/suppl/GSM347165.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347166 | GPL570 |
|
In vivo sFRP1_1
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
| Sample_geo_accession | GSM347166
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347166/suppl/GSM347166.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347167 | GPL570 |
|
In vivo sFRP1_2
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
| Sample_geo_accession | GSM347167
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347167/suppl/GSM347167.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347168 | GPL570 |
|
In vivo sFRP1_3
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
| Sample_geo_accession | GSM347168
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347168/suppl/GSM347168.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347169 | GPL570 |
|
In vivo sFRP1_4
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
| Sample_geo_accession | GSM347169
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347169/suppl/GSM347169.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347170 | GPL570 |
|
In vivo sFRP1_5
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
| Sample_geo_accession | GSM347170
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347170/suppl/GSM347170.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347171 | GPL570 |
|
In vivo sFRP1_6
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/sFRP1 xenograft
|
| Sample_geo_accession | GSM347171
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347171/suppl/GSM347171.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347172 | GPL570 |
|
In vivo control_1
|
tumor lysate from MDA-MB-231/control xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/control xenograft
|
| Sample_geo_accession | GSM347172
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347172/suppl/GSM347172.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347173 | GPL570 |
|
In vivo control_2
|
tumor lysate from MDA-MB-231/control xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/control xenograft
|
| Sample_geo_accession | GSM347173
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347173/suppl/GSM347173.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347174 | GPL570 |
|
In vivo control_3
|
tumor lysate from MDA-MB-231/control xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/control xenograft
|
| Sample_geo_accession | GSM347174
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347174/suppl/GSM347174.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347175 | GPL570 |
|
In vivo control_4
|
tumor lysate from MDA-MB-231/control xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/control xenograft
|
| Sample_geo_accession | GSM347175
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347175/suppl/GSM347175.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
|
GSM347176 | GPL570 |
|
In vivo control_5
|
tumor lysate from MDA-MB-231/control xenograft
|
Strain: Balb/c nude, Sex: female, Tissue: xenografted mammary tumor
|
tumor lysate from MDA-MB-231/control xenograft
|
| Sample_geo_accession | GSM347176
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Dec 03 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | RNA was taken from cell lysates and tumor lysates
| | Sample_growth_protocol_ch1 | Three MDA-MB-231/sFRP1 clones and three MDA-MB-231/control clones were cultured in DMEM 10% FCS with 1mg/ml G-418. In parallel, the three sFRP1+ clones and the three control clones were pooled and injected to fat pads of nude mice. A few – several weeks after, mice were sacrificed and tumors arising from these cells were taken to isolate RNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cultured cells were collected when plates were 70-80% confluent and RNA was extracted using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). To extract the RNA from tumours, dissected tumours were put in RNAlater (Qiagen) over night at 4°C, followed by RNA extraction using TRIzol reagent (Invitrogen) and washing using the RNAeasy Mini kit according to manufacturer’s instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Affymetrix protocol
| | Sample_data_processing | GCRMA generated using Genedata's Refiner 5.0 software tool
| | Sample_platform_id | GPL570
| | Sample_contact_name | Edward,,Oakeley
| | Sample_contact_institute | FMI
| | Sample_contact_address | Maulbeerstrasse 66
| | Sample_contact_city | Basel
| | Sample_contact_zip/postal_code | 4058
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347176/suppl/GSM347176.CEL.gz
| | Sample_series_id | GSE13806
| | Sample_data_row_count | 54675
| | |
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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