Search results for the GEO ID: GSE13807  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM347180 | GPL1261 |
|
WT_1
|
Wild type group 1
|
wild type
|
WT_1
|
| Sample_geo_accession | GSM347180
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347180/suppl/GSM347180.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347181 | GPL1261 |
|
MUT_1
|
Mutant group 1
|
mutant
|
MUT_1
|
| Sample_geo_accession | GSM347181
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347181/suppl/GSM347181.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347182 | GPL1261 |
|
WT_2
|
Wild type group 2
|
wild type
|
WT_2
|
| Sample_geo_accession | GSM347182
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347182/suppl/GSM347182.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347183 | GPL1261 |
|
MUT_2
|
Mutant group 2
|
mutant
|
MUT_2
|
| Sample_geo_accession | GSM347183
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347183/suppl/GSM347183.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347184 | GPL1261 |
|
WT_3
|
Wild type group 3
|
wild type
|
WT_3
|
| Sample_geo_accession | GSM347184
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347184/suppl/GSM347184.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347185 | GPL1261 |
|
MUT_3
|
Mutant group 3
|
mutant
|
MUT_3
|
| Sample_geo_accession | GSM347185
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347185/suppl/GSM347185.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347186 | GPL1261 |
|
WT_4
|
Wild type group 4
|
wild type
|
WT_4
|
| Sample_geo_accession | GSM347186
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347186/suppl/GSM347186.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347187 | GPL1261 |
|
MUT_4
|
Mutant group 4
|
mutant
|
MUT_4
|
| Sample_geo_accession | GSM347187
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347187/suppl/GSM347187.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347188 | GPL1261 |
|
WT_5
|
Wild type group 5
|
wild type
|
WT_5
|
| Sample_geo_accession | GSM347188
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347188/suppl/GSM347188.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
GSM347189 | GPL1261 |
|
MUT_5
|
Mutant group 5
|
mutant
|
MUT_5
|
| Sample_geo_accession | GSM347189
| | Sample_status | Public on Jan 31 2011
| | Sample_submission_date | Dec 03 2008
| | Sample_last_update_date | Jan 31 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Pregent moms were cevericaly dislocated and embryos dissected in PBS. Limbs were cut off of embryos for RNA processing.
| | Sample_growth_protocol_ch1 | Mice were grown in the SPF facilty at the UF Cancer/Genetics Research Complex
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was harvested using the RNeasy RNA Isolation kit standard protocol (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual rev 3, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station FS 4500.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner G3000
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS 1.3) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | M Cecilia,,Lopez
| | Sample_contact_email | mclopez@ufl.edu
| | Sample_contact_phone | 352 273-8133
| | Sample_contact_fax | 352 273-8284
| | Sample_contact_laboratory | Microarray lab 103
| | Sample_contact_department | Molecular Genetics and Microbiology
| | Sample_contact_institute | University of Florida
| | Sample_contact_address | 1376 Mowry Road
| | Sample_contact_city | Gainesville
| | Sample_contact_state | FL
| | Sample_contact_zip/postal_code | 32610-3610
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347189/suppl/GSM347189.CEL.gz
| | Sample_series_id | GSE13807
| | Sample_data_row_count | 45101
| | |
|
| |
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