Search results for the GEO ID: GSE13811 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM347177 | GPL570 |
|
CLL_1_fresh
|
purified CLL cells, patient 1, fresh
|
purified CLL cells, patient 1, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347177
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347177/suppl/GSM347177.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347177/suppl/GSM347177.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347178 | GPL570 |
|
CLL_1_14dNLC
|
purified CLL cells, patient 1 after 14 days co-culture with Nurse-like cells (NLC)
|
purified CLL cells, patient 1 after 14 days co-culture with Nurse-like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347178
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347178/suppl/GSM347178.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347178/suppl/GSM347178.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347179 | GPL570 |
|
CLL_2_fresh
|
purified CLL cells, patient 2, fresh
|
purified CLL cells, patient 2, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347179
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347179/suppl/GSM347179.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347179/suppl/GSM347179.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347190 | GPL570 |
|
CLL_2_14d_NLC
|
purified CLL cells, patient 2 after 14 days co-culture with Nurse-like cells (NLC)
|
purified CLL cells, patient 2 after 14 days co-culture with Nurse-like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347190
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347190/suppl/GSM347190.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347190/suppl/GSM347190.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347192 | GPL570 |
|
CLL_3_14dNLC
|
purified CLL cells, patient 3 after 14days co-culture with Nurse like cells (NLC)
|
purified CLL cells, patient 3 after 14days co-culture with Nurse like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347192
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347192/suppl/GSM347192.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347192/suppl/GSM347192.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347195 | GPL570 |
|
CLL_3_fresh
|
purified CLL cells, patient 3, fresh
|
purified CLL cells, patient 3, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347195
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347195/suppl/GSM347195.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347195/suppl/GSM347195.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347329 | GPL570 |
|
CLL_4_14dNLC
|
purified CLL cells, patient 4 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells, patient 4 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347329
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347329/suppl/GSM347329.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347329/suppl/GSM347329.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347331 | GPL570 |
|
CLL_4_fresh
|
purified CLL cells, patient 4, fresh
|
purified CLL cells, patient 4, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347331
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347331/suppl/GSM347331.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347331/suppl/GSM347331.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347333 | GPL570 |
|
CLL_5_fresh
|
purified CLL cells, patient 5, fresh
|
purified CLL cells, patient 5, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347333
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347333/suppl/GSM347333.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347333/suppl/GSM347333.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347334 | GPL570 |
|
CLL_5_14dNLC
|
purified CLL cells, patient 5 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells, patient 5 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347334
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347334/suppl/GSM347334.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347334/suppl/GSM347334.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347336 | GPL570 |
|
CLL_6_fresh
|
purified CLL cells, patient 6, fresh
|
purified CLL cells, patient 6, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347336
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347336/suppl/GSM347336.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347336/suppl/GSM347336.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347338 | GPL570 |
|
CLL_6_14dNLC
|
purified CLL cells, patient 6 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells, patient 6 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347338
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347338/suppl/GSM347338.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347338/suppl/GSM347338.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347339 | GPL570 |
|
CLL_7_fresh
|
purified CLL cells, patient 7, fresh
|
purified CLL cells, patient 7, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347339
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347339/suppl/GSM347339.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347339/suppl/GSM347339.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347341 | GPL570 |
|
CLL_7_14dNLC
|
purified CLL cells, patient 7 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells, patient 7 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347341
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347341/suppl/GSM347341.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347341/suppl/GSM347341.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347343 | GPL570 |
|
CLL_8_fresh
|
purified CLL cells, patient 8, fresh
|
purified CLL cells, patient 8, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347343
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347343/suppl/GSM347343.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347343/suppl/GSM347343.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347344 | GPL570 |
|
CLL_8_14dNLC
|
purified CLL cells, patient 8 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells, patient 8 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347344
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347344/suppl/GSM347344.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347344/suppl/GSM347344.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347345 | GPL570 |
|
CLL_9_fresh
|
purified CLL cells, patient 9, fresh
|
purified CLL cells, patient 9, fresh
|
purified CLL cells from peripheral blood
|
Sample_geo_accession | GSM347345
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347345/suppl/GSM347345.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347345/suppl/GSM347345.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
GSM347346 | GPL570 |
|
CLL_9_14dNLC
|
purified CLL cells, patient 9 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells, patient 9 after 14d co-culture with Nurse like cells (NLC)
|
purified CLL cells from peripheral blood after 14 days co-culture with Nurse-like cells (NLC)
|
Sample_geo_accession | GSM347346
| Sample_status | Public on Dec 11 2008
| Sample_submission_date | Dec 03 2008
| Sample_last_update_date | Dec 04 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Using TRIzol from Invitrogen, purified B cells were lysed according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Total RNA extraction then was performed with the PureLink Micro-to-Midi Total RNA Purification System as described by the manufacturer (Invitrogen).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix Labeling Protocol.
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microgr total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Affymetrix Hybridization Protocol.
| Sample_hyb_protocol | Following fragmentation, 20 microgr of cRNA were hybridized for 16 hours at 45°C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | Affymetrix Scanning Protocol.
| Sample_scan_protocol | Scanning was performed by the Affymetrix 3000 Scanner.
| Sample_data_processing | MAS 5.0 Data Processing Protocol
| Sample_data_processing | Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. The average probe array signals were scaled to a target signal of 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Andreas,,Rosenwald
| Sample_contact_email | Rosenwald@mail.uni-wuerzburg.de
| Sample_contact_phone | 0931-201-47424
| Sample_contact_fax | 0931-201-47440
| Sample_contact_laboratory | Rosenwald-Lab
| Sample_contact_department | Institute of Pathology
| Sample_contact_institute | University of Wuerzburg
| Sample_contact_address | Josef-Schneider-Strasse 2
| Sample_contact_city | Wuerzburg
| Sample_contact_zip/postal_code | 97080
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347346/suppl/GSM347346.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM347nnn/GSM347346/suppl/GSM347346.CHP.gz
| Sample_series_id | GSE13811
| Sample_data_row_count | 54675
| |
|
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