Search results for the GEO ID: GSE14103  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM353359 | GPL570 |
|
Synchronized HCT116_DMSO_0h
|
Synchronized HCT116, DMSO, 0h
|
Cell line: HCT116
Treatment: DMSO
Time course: 0h
|
N/A
|
| Sample_geo_accession | GSM353359
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353359/suppl/GSM353359.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353360 | GPL570 |
|
Synchronized HCT116_DMSO_2h
|
Synchronized HCT116, DMSO, 2h
|
Cell line: HCT116
Treatment: DMSO
Time course: 2h
|
N/A
|
| Sample_geo_accession | GSM353360
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353360/suppl/GSM353360.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353424 | GPL570 |
|
Synchronized HCT116_DMSO_4h
|
Synchronized HCT116, DMSO, 4h
|
Cell line: HCT116
Treatment: DMSO
Time course: 4h
|
N/A
|
| Sample_geo_accession | GSM353424
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353424/suppl/GSM353424.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353425 | GPL570 |
|
Synchronized HCT116_DMSO_6h
|
Synchronized HCT116, DMSO, 6h
|
Cell line: HCT116
Treatment: DMSO
Time course: 6h
|
N/A
|
| Sample_geo_accession | GSM353425
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353425/suppl/GSM353425.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353426 | GPL570 |
|
Synchronized HCT116_DMSO_7h
|
Synchronized HCT116, DMSO, 7h
|
Cell line: HCT116
Treatment: DMSO
Time course: 7h
|
N/A
|
| Sample_geo_accession | GSM353426
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353426/suppl/GSM353426.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353427 | GPL570 |
|
Synchronized HCT116_DMSO_8h
|
Synchronized HCT116, DMSO, 8h
|
Cell line: HCT116
Treatment: DMSO
Time course: 8h
|
N/A
|
| Sample_geo_accession | GSM353427
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353427/suppl/GSM353427.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353428 | GPL570 |
|
Synchronized HCT116_DMSO_9h
|
Synchronized HCT116, DMSO, 9h
|
Cell line: HCT116
Treatment: DMSO
Time course: 9h
|
N/A
|
| Sample_geo_accession | GSM353428
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353428/suppl/GSM353428.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353429 | GPL570 |
|
Synchronized HCT116_DMSO_10h
|
Synchronized HCT116, DMSO, 10h
|
Cell line: HCT116
Treatment: DMSO
Time course: 10h
|
N/A
|
| Sample_geo_accession | GSM353429
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353429/suppl/GSM353429.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353430 | GPL570 |
|
Synchronized HCT116_Nocodazole_7h
|
Synchronized HCT116, Nocodazole, 7h
|
Cell line: HCT116
Treatment: Nocodazole
Time course: 7h
|
N/A
|
| Sample_geo_accession | GSM353430
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353430/suppl/GSM353430.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353431 | GPL570 |
|
Synchronized HCT116_Nocodazole_8h
|
Synchronized HCT116, Nocodazole, 8h
|
Cell line: HCT116
Treatment: Nocodazole
Time course: 8h
|
N/A
|
| Sample_geo_accession | GSM353431
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353431/suppl/GSM353431.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353433 | GPL570 |
|
Synchronized HCT116_Nocodazole_9h
|
Synchronized HCT116, Nocodazole, 9h
|
Cell line: HCT116
Treatment: Nocodazole
Time course: 9h
|
N/A
|
| Sample_geo_accession | GSM353433
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353433/suppl/GSM353433.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
GSM353434 | GPL570 |
|
Synchronized HCT116_Nocodazole_10h
|
Synchronized HCT116, Nocodazole, 10h
|
Cell line: HCT116
Treatment: Nocodazole
Time course: 10h
|
N/A
|
| Sample_geo_accession | GSM353434
| | Sample_status | Public on Mar 31 2009
| | Sample_submission_date | Dec 22 2008
| | Sample_last_update_date | Mar 31 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9-h incubation in normal medium and a second 16-h incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9, and 10 h as a control or 0.1 mg/ml nocodazole (Sigma-Aldrich) for 7, 8, 9, and 10 h.
| | Sample_growth_protocol_ch1 | The HCT116 colorectal cancer cell line (ATCC) was grown in McCoy's 5A medium modified (Sigma-Aldrich) with 10% FBS (JBS) and maintained at 37°C and 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated with RNeasy kits (Qiagen) according to the manufacturers' instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the manufacturer's instructions (Affymetrix Expression Analysis Technical Manual, Rev. 4).
| | Sample_hyb_protocol | The cRNA products were fragmented, heated at 99 degrees C for 5 min and hybridized for 16 h at 45 degrees C. The microarrays were then washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain.
| | Sample_scan_protocol | Scanning was carried out using the GeneChip Scanner and image analysis was performed using GeneChip Operating Software.
| | Sample_data_processing | GCRMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hideaki,,Mizuno
| | Sample_contact_email | mizunohda@chugai-pharm.co.jp
| | Sample_contact_laboratory | Kamakura research labs.
| | Sample_contact_department | Discovery research dept.
| | Sample_contact_institute | Chugai Pharmaceutical Co., Ltd.
| | Sample_contact_address | 200 Kajiwara
| | Sample_contact_city | Kamakura
| | Sample_contact_state | Kanagawa
| | Sample_contact_zip/postal_code | 247-8530
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://researchmap.jp/mizuno
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM353nnn/GSM353434/suppl/GSM353434.CEL.gz
| | Sample_series_id | GSE14103
| | Sample_data_row_count | 54675
| | |
|
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