Search results for the GEO ID: GSE14211 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM355342 | GPL1261 |
|
Noto-GFP_positive_sort_rep1
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Noto-GFP positive cells sorted from the posterior of E8.5 mouse embryos
|
STRAIN: 129S3;ICR
GENDER: MIXED
|
replicate 1: posterior regions dissected and pooled from 23 embryos; ~2600 Noto-GFP positive cells sorted
|
Sample_geo_accession | GSM355342
| Sample_status | Public on Oct 01 2011
| Sample_submission_date | Dec 24 2008
| Sample_last_update_date | Oct 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The region posterior to the SI-SII somite boundary was dissected, pooled, and dissociated using 1% trypsin in PBS. Noto-GFP+ cells were sorted from Noto-GFP- cells and both populations were collected (~2600-6600 cells per experiment)
| Sample_growth_protocol_ch1 | Litters of heterozygous Noto-GFP embryos (mixed 129S3;ICR background) were collected at embryonic day (E) 8.5 (median somite number 6; ~25-50 embryos per experiment)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Small-scale RNA extraction was performed using Trizol or Trizol LS as per the manufacturer's instructions. Sigma GeneElute linear polyacrylamide was used as carrier
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was amplified and labeled using the AFFYMETRIX GeneChip® Two-Cycle Target Labeling Kit
| Sample_hyb_protocol | Samples were hybridized to Affymetrix MOE430v2 arrays using the manufacturer's protocols
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GeneChip Scanner 3000
| Sample_data_processing | Data was analyzed using GCOS software v1.4
| Sample_platform_id | GPL1261
| Sample_contact_name | Owen,James,Tamplin
| Sample_contact_email | otamplin@enders.tch.harvard.edu
| Sample_contact_fax | 416-813-5252
| Sample_contact_laboratory | Rossant Lab
| Sample_contact_department | Program in Developmental and Stem Cell Biology
| Sample_contact_institute | The Hospital for Sick Children Research Institute
| Sample_contact_address | TMDT, Room 13-305, 101 College Street
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_contact_web_link | http://www.sickkids.ca/rossant/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355342/suppl/GSM355342.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355342/suppl/GSM355342.CHP.gz
| Sample_series_id | GSE14211
| Sample_data_row_count | 45101
| |
|
GSM355343 | GPL1261 |
|
Noto-GFP_positive_sort_rep2
|
Noto-GFP positive cells sorted from the posterior of E8.5 mouse embryos
|
STRAIN: 129S3;ICR
GENDER: MIXED
|
replicate 2: posterior regions dissected and pooled from 39 embryos; ~4300 Noto-GFP positive cells sorted
|
Sample_geo_accession | GSM355343
| Sample_status | Public on Oct 01 2011
| Sample_submission_date | Dec 24 2008
| Sample_last_update_date | Oct 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The region posterior to the SI-SII somite boundary was dissected, pooled, and dissociated using 1% trypsin in PBS. Noto-GFP+ cells were sorted from Noto-GFP- cells and both populations were collected (~2600-6600 cells per experiment)
| Sample_growth_protocol_ch1 | Litters of heterozygous Noto-GFP embryos (mixed 129S3;ICR background) were collected at embryonic day (E) 8.5 (median somite number 6; ~25-50 embryos per experiment)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Small-scale RNA extraction was performed using Trizol or Trizol LS as per the manufacturer's instructions. Sigma GeneElute linear polyacrylamide was used as carrier
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was amplified and labeled using the AFFYMETRIX GeneChip® Two-Cycle Target Labeling Kit
| Sample_hyb_protocol | Samples were hybridized to Affymetrix MOE430v2 arrays using the manufacturer's protocols
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GeneChip Scanner 3000
| Sample_data_processing | Data was analyzed using GCOS software v1.4
| Sample_platform_id | GPL1261
| Sample_contact_name | Owen,James,Tamplin
| Sample_contact_email | otamplin@enders.tch.harvard.edu
| Sample_contact_fax | 416-813-5252
| Sample_contact_laboratory | Rossant Lab
| Sample_contact_department | Program in Developmental and Stem Cell Biology
| Sample_contact_institute | The Hospital for Sick Children Research Institute
| Sample_contact_address | TMDT, Room 13-305, 101 College Street
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_contact_web_link | http://www.sickkids.ca/rossant/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355343/suppl/GSM355343.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355343/suppl/GSM355343.CHP.gz
| Sample_series_id | GSE14211
| Sample_data_row_count | 45101
| |
|
GSM355344 | GPL1261 |
|
Noto-GFP_positive_sort_rep3
|
Noto-GFP positive cells sorted from the posterior of E8.5 mouse embryos
|
STRAIN: 129S3;ICR
GENDER: MIXED
|
replicate 3: posterior regions dissected and pooled from 52 embryos; ~6600 Noto-GFP positive cells sorted
|
Sample_geo_accession | GSM355344
| Sample_status | Public on Oct 01 2011
| Sample_submission_date | Dec 24 2008
| Sample_last_update_date | Oct 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The region posterior to the SI-SII somite boundary was dissected, pooled, and dissociated using 1% trypsin in PBS. Noto-GFP+ cells were sorted from Noto-GFP- cells and both populations were collected (~2600-6600 cells per experiment)
| Sample_growth_protocol_ch1 | Litters of heterozygous Noto-GFP embryos (mixed 129S3;ICR background) were collected at embryonic day (E) 8.5 (median somite number 6; ~25-50 embryos per experiment)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Small-scale RNA extraction was performed using Trizol or Trizol LS as per the manufacturer's instructions. Sigma GeneElute linear polyacrylamide was used as carrier
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was amplified and labeled using the AFFYMETRIX GeneChip® Two-Cycle Target Labeling Kit
| Sample_hyb_protocol | Samples were hybridized to Affymetrix MOE430v2 arrays using the manufacturer's protocols
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GeneChip Scanner 3000
| Sample_data_processing | Data was analyzed using GCOS software v1.4
| Sample_platform_id | GPL1261
| Sample_contact_name | Owen,James,Tamplin
| Sample_contact_email | otamplin@enders.tch.harvard.edu
| Sample_contact_fax | 416-813-5252
| Sample_contact_laboratory | Rossant Lab
| Sample_contact_department | Program in Developmental and Stem Cell Biology
| Sample_contact_institute | The Hospital for Sick Children Research Institute
| Sample_contact_address | TMDT, Room 13-305, 101 College Street
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_contact_web_link | http://www.sickkids.ca/rossant/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355344/suppl/GSM355344.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355344/suppl/GSM355344.CHP.gz
| Sample_series_id | GSE14211
| Sample_data_row_count | 45101
| |
|
GSM355345 | GPL1261 |
|
Noto-GFP_negative_sort_rep1
|
GFP negative cells sorted from the posterior of E8.5 mouse embryos
|
STRAIN: 129S3;ICR
GENDER: MIXED
|
replicate 1: posterior regions dissected and pooled from 23 embryos
|
Sample_geo_accession | GSM355345
| Sample_status | Public on Oct 01 2011
| Sample_submission_date | Dec 24 2008
| Sample_last_update_date | Oct 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The region posterior to the SI-SII somite boundary was dissected, pooled, and dissociated using 1% trypsin in PBS. Noto-GFP+ cells were sorted from Noto-GFP- cells and both populations were collected (~2600-6600 cells per experiment)
| Sample_growth_protocol_ch1 | Litters of heterozygous Noto-GFP embryos (mixed 129S3;ICR background) were collected at embryonic day (E) 8.5 (median somite number 6; ~25-50 embryos per experiment)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Small-scale RNA extraction was performed using Trizol or Trizol LS as per the manufacturer's instructions. Sigma GeneElute linear polyacrylamide was used as carrier
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was amplified and labeled using the AFFYMETRIX GeneChip® Two-Cycle Target Labeling Kit
| Sample_hyb_protocol | Samples were hybridized to Affymetrix MOE430v2 arrays using the manufacturer's protocols
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GeneChip Scanner 3000
| Sample_data_processing | Data was analyzed using GCOS software v1.4
| Sample_platform_id | GPL1261
| Sample_contact_name | Owen,James,Tamplin
| Sample_contact_email | otamplin@enders.tch.harvard.edu
| Sample_contact_fax | 416-813-5252
| Sample_contact_laboratory | Rossant Lab
| Sample_contact_department | Program in Developmental and Stem Cell Biology
| Sample_contact_institute | The Hospital for Sick Children Research Institute
| Sample_contact_address | TMDT, Room 13-305, 101 College Street
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_contact_web_link | http://www.sickkids.ca/rossant/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355345/suppl/GSM355345.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355345/suppl/GSM355345.CHP.gz
| Sample_series_id | GSE14211
| Sample_data_row_count | 45101
| |
|
GSM355346 | GPL1261 |
|
Noto-GFP_negative_sort_rep2
|
GFP negative cells sorted from the posterior of E8.5 mouse embryos
|
STRAIN: 129S3;ICR
GENDER: MIXED
|
replicate 2: posterior regions dissected and pooled from 39 embryos
|
Sample_geo_accession | GSM355346
| Sample_status | Public on Oct 01 2011
| Sample_submission_date | Dec 24 2008
| Sample_last_update_date | Oct 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The region posterior to the SI-SII somite boundary was dissected, pooled, and dissociated using 1% trypsin in PBS. Noto-GFP+ cells were sorted from Noto-GFP- cells and both populations were collected (~2600-6600 cells per experiment)
| Sample_growth_protocol_ch1 | Litters of heterozygous Noto-GFP embryos (mixed 129S3;ICR background) were collected at embryonic day (E) 8.5 (median somite number 6; ~25-50 embryos per experiment)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Small-scale RNA extraction was performed using Trizol or Trizol LS as per the manufacturer's instructions. Sigma GeneElute linear polyacrylamide was used as carrier
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was amplified and labeled using the AFFYMETRIX GeneChip® Two-Cycle Target Labeling Kit
| Sample_hyb_protocol | Samples were hybridized to Affymetrix MOE430v2 arrays using the manufacturer's protocols
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GeneChip Scanner 3000
| Sample_data_processing | Data was analyzed using GCOS software v1.4
| Sample_platform_id | GPL1261
| Sample_contact_name | Owen,James,Tamplin
| Sample_contact_email | otamplin@enders.tch.harvard.edu
| Sample_contact_fax | 416-813-5252
| Sample_contact_laboratory | Rossant Lab
| Sample_contact_department | Program in Developmental and Stem Cell Biology
| Sample_contact_institute | The Hospital for Sick Children Research Institute
| Sample_contact_address | TMDT, Room 13-305, 101 College Street
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_contact_web_link | http://www.sickkids.ca/rossant/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355346/suppl/GSM355346.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355346/suppl/GSM355346.CHP.gz
| Sample_series_id | GSE14211
| Sample_data_row_count | 45101
| |
|
GSM355347 | GPL1261 |
|
Noto-GFP_negative_sort_rep3
|
GFP negative cells sorted from the posterior of E8.5 mouse embryos
|
STRAIN: 129S3;ICR
GENDER: MIXED
|
replicate 3: posterior regions dissected and pooled from 52 embryos
|
Sample_geo_accession | GSM355347
| Sample_status | Public on Oct 01 2011
| Sample_submission_date | Dec 24 2008
| Sample_last_update_date | Oct 01 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The region posterior to the SI-SII somite boundary was dissected, pooled, and dissociated using 1% trypsin in PBS. Noto-GFP+ cells were sorted from Noto-GFP- cells and both populations were collected (~2600-6600 cells per experiment)
| Sample_growth_protocol_ch1 | Litters of heterozygous Noto-GFP embryos (mixed 129S3;ICR background) were collected at embryonic day (E) 8.5 (median somite number 6; ~25-50 embryos per experiment)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Small-scale RNA extraction was performed using Trizol or Trizol LS as per the manufacturer's instructions. Sigma GeneElute linear polyacrylamide was used as carrier
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA was amplified and labeled using the AFFYMETRIX GeneChip® Two-Cycle Target Labeling Kit
| Sample_hyb_protocol | Samples were hybridized to Affymetrix MOE430v2 arrays using the manufacturer's protocols
| Sample_scan_protocol | Arrays were scanned using an Affymetrix GeneChip Scanner 3000
| Sample_data_processing | Data was analyzed using GCOS software v1.4
| Sample_platform_id | GPL1261
| Sample_contact_name | Owen,James,Tamplin
| Sample_contact_email | otamplin@enders.tch.harvard.edu
| Sample_contact_fax | 416-813-5252
| Sample_contact_laboratory | Rossant Lab
| Sample_contact_department | Program in Developmental and Stem Cell Biology
| Sample_contact_institute | The Hospital for Sick Children Research Institute
| Sample_contact_address | TMDT, Room 13-305, 101 College Street
| Sample_contact_city | Toronto
| Sample_contact_state | Ontario
| Sample_contact_zip/postal_code | M5G 1L7
| Sample_contact_country | Canada
| Sample_contact_web_link | http://www.sickkids.ca/rossant/
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355347/suppl/GSM355347.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM355nnn/GSM355347/suppl/GSM355347.CHP.gz
| Sample_series_id | GSE14211
| Sample_data_row_count | 45101
| |
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