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Search results for the GEO ID: GSE14222
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GSM ID
GPL ID
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Title
Source name
Description
Characteristics
GSM356341
GPL1261
Mouse Testis Thy1 Negative Fraction Rep 1 Mouse Testis 6 Days Old C57BL/6 Donor Mouse, MACS Isolated Thy1 Negative Fraction, 1st Replicate Sample Affymetrix microarray suite 5.0 was used to quantitate expression levels for targeted genes; default values provided by Affymetrix were applied to all analysis parameters. The number of probe pairs meeting the default discrimination threshold (t = 0.015) was used to assign a call of absent, present, or marginal for each assayed gene, and a P value was calculated to reflect confidence in the detection call. A weighted mean of probe fluorescence (corrected for nonspecific signal by subtracting the mismatch probe value) was calculated by using the One-step Tukey’s Biweight Estimate. This Signal value, a relative measure of the expression level, was computed for each assayed gene. Global scaling was applied to allow comparison of gene signals across multiple microarrays: After exclusion of the highest and lowest 2%, the average total chip signal was calculated and used to determine what scaling factor was required to adjust the chip average to an arbitrary target of 150. All signal values from one microarray were then multiplied by the appropriate scaling factor.
GSM356342
GPL1261
Mouse Testis Thy1 Negative Fraction Rep 2 Mouse Testis 6 Days Old C57BL/6 Donor Mouse, MACS Isolated Thy1 Negative Fraction, 2nd Replicate Sample Affymetrix microarray suite 5.0 was used to quantitate expression levels for targeted genes; default values provided by Affymetrix were applied to all analysis parameters. The number of probe pairs meeting the default discrimination threshold (t = 0.015) was used to assign a call of absent, present, or marginal for each assayed gene, and a P value was calculated to reflect confidence in the detection call. A weighted mean of probe fluorescence (corrected for nonspecific signal by subtracting the mismatch probe value) was calculated by using the One-step Tukey’s Biweight Estimate. This Signal value, a relative measure of the expression level, was computed for each assayed gene. Global scaling was applied to allow comparison of gene signals across multiple microarrays: After exclusion of the highest and lowest 2%, the average total chip signal was calculated and used to determine what scaling factor was required to adjust the chip average to an arbitrary target of 150. All signal values from one microarray were then multiplied by the appropriate scaling factor.
GSM356343
GPL1261
Mouse Testis Thy1 Negative Fraction Rep 3 Mouse Testis 6 Days Old C57BL/6 Donor Mouse, MACS Isolated Thy1 Negative Fraction, 3rd Replicate Sample Affymetrix microarray suite 5.0 was used to quantitate expression levels for targeted genes; default values provided by Affymetrix were applied to all analysis parameters. The number of probe pairs meeting the default discrimination threshold (t = 0.015) was used to assign a call of absent, present, or marginal for each assayed gene, and a P value was calculated to reflect confidence in the detection call. A weighted mean of probe fluorescence (corrected for nonspecific signal by subtracting the mismatch probe value) was calculated by using the One-step Tukey’s Biweight Estimate. This Signal value, a relative measure of the expression level, was computed for each assayed gene. Global scaling was applied to allow comparison of gene signals across multiple microarrays: After exclusion of the highest and lowest 2%, the average total chip signal was calculated and used to determine what scaling factor was required to adjust the chip average to an arbitrary target of 150. All signal values from one microarray were then multiplied by the appropriate scaling factor.
GSM356344
GPL1261
Mouse Testis Thy1 Positive Fraction Rep1 Mouse Testis 6 Days Old C57BL/6 Donor Mouse, MACS Isolated Thy1 Positive Fraction, 1st Replicate Sample Affymetrix microarray suite 5.0 was used to quantitate expression levels for targeted genes; default values provided by Affymetrix were applied to all analysis parameters. The number of probe pairs meeting the default discrimination threshold (t = 0.015) was used to assign a call of absent, present, or marginal for each assayed gene, and a P value was calculated to reflect confidence in the detection call. A weighted mean of probe fluorescence (corrected for nonspecific signal by subtracting the mismatch probe value) was calculated by using the One-step Tukey’s Biweight Estimate. This Signal value, a relative measure of the expression level, was computed for each assayed gene. Global scaling was applied to allow comparison of gene signals across multiple microarrays: After exclusion of the highest and lowest 2%, the average total chip signal was calculated and used to determine what scaling factor was required to adjust the chip average to an arbitrary target of 150. All signal values from one microarray were then multiplied by the appropriate scaling factor.
GSM356345
GPL1261
Mouse Testis Thy1 Positive Fraction Rep2 Mouse Testis 6 Days Old C57BL/6 Donor Mouse, MACS Isolated Thy1 Positive Fraction, 2nd Replicate Sample Affymetrix microarray suite 5.0 was used to quantitate expression levels for targeted genes; default values provided by Affymetrix were applied to all analysis parameters. The number of probe pairs meeting the default discrimination threshold (t = 0.015) was used to assign a call of absent, present, or marginal for each assayed gene, and a P value was calculated to reflect confidence in the detection call. A weighted mean of probe fluorescence (corrected for nonspecific signal by subtracting the mismatch probe value) was calculated by using the One-step Tukey’s Biweight Estimate. This Signal value, a relative measure of the expression level, was computed for each assayed gene. Global scaling was applied to allow comparison of gene signals across multiple microarrays: After exclusion of the highest and lowest 2%, the average total chip signal was calculated and used to determine what scaling factor was required to adjust the chip average to an arbitrary target of 150. All signal values from one microarray were then multiplied by the appropriate scaling factor.
GSM356346
GPL1261
Mouse Testis Thy1 Positive Fraction Rep3 Mouse Testis 6 Days Old C57BL/6 Donor Mouse, MACS Isolated Thy1 Positive Fraction, 3rd Replicate Sample Affymetrix microarray suite 5.0 was used to quantitate expression levels for targeted genes; default values provided by Affymetrix were applied to all analysis parameters. The number of probe pairs meeting the default discrimination threshold (t = 0.015) was used to assign a call of absent, present, or marginal for each assayed gene, and a P value was calculated to reflect confidence in the detection call. A weighted mean of probe fluorescence (corrected for nonspecific signal by subtracting the mismatch probe value) was calculated by using the One-step Tukey’s Biweight Estimate. This Signal value, a relative measure of the expression level, was computed for each assayed gene. Global scaling was applied to allow comparison of gene signals across multiple microarrays: After exclusion of the highest and lowest 2%, the average total chip signal was calculated and used to determine what scaling factor was required to adjust the chip average to an arbitrary target of 150. All signal values from one microarray were then multiplied by the appropriate scaling factor.
 
 
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