Search results for the GEO ID: GSE14226  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM356505 | GPL339 |
|
Hunk Knock-Out rep 1
|
Hunk Knock-Out, Mammary gland
|
Genotype: MMTV-c-myc/Hunk KO; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk KO genotype
|
| Sample_geo_accession | GSM356505
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356505/suppl/GSM356505.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356506 | GPL339 |
|
Hunk Knock-Out rep 2
|
Hunk Knock-Out, Mammary gland
|
Genotype: MMTV-c-myc/Hunk KO; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk KO genotype
|
| Sample_geo_accession | GSM356506
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356506/suppl/GSM356506.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356507 | GPL339 |
|
Hunk Knock-Out rep 3
|
Hunk Knock-Out, Mammary gland
|
Genotype: MMTV-c-myc/Hunk KO; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk KO genotype
|
| Sample_geo_accession | GSM356507
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356507/suppl/GSM356507.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356508 | GPL339 |
|
Hunk Knock-Out rep 4
|
Hunk Knock-Out, Mammary gland
|
Genotype: MMTV-c-myc/Hunk KO; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk KO genotype
|
| Sample_geo_accession | GSM356508
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356508/suppl/GSM356508.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356509 | GPL339 |
|
Hunk Knock-Out rep 5
|
Hunk Knock-Out, Mammary gland
|
Genotype: MMTV-c-myc/Hunk KO; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk KO genotype
|
| Sample_geo_accession | GSM356509
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356509/suppl/GSM356509.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356510 | GPL339 |
|
Hunk Knock-Out rep 6
|
Hunk Knock-Out, Mammary gland
|
Genotype: MMTV-c-myc/Hunk KO; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk KO genotype
|
| Sample_geo_accession | GSM356510
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356510/suppl/GSM356510.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356511 | GPL339 |
|
Hunk WT rep1
|
Hunk WT, Mammary gland
|
Genotype: MMTV-c-myc/Hunk WT; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk WT genotype
|
| Sample_geo_accession | GSM356511
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356511/suppl/GSM356511.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356512 | GPL339 |
|
Hunk WT rep2
|
Hunk WT, Mammary gland
|
Genotype: MMTV-c-myc/Hunk WT; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk WT genotype
|
| Sample_geo_accession | GSM356512
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356512/suppl/GSM356512.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356513 | GPL339 |
|
Hunk WT rep3
|
Hunk WT, Mammary gland
|
Genotype: MMTV-c-myc/Hunk WT; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk WT genotype
|
| Sample_geo_accession | GSM356513
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356513/suppl/GSM356513.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356514 | GPL339 |
|
Hunk WT rep4
|
Hunk WT, Mammary gland
|
Genotype: MMTV-c-myc/Hunk WT; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk WT genotype
|
| Sample_geo_accession | GSM356514
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356514/suppl/GSM356514.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356515 | GPL339 |
|
Hunk WT rep5
|
Hunk WT, Mammary gland
|
Genotype: MMTV-c-myc/Hunk WT; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk WT genotype
|
| Sample_geo_accession | GSM356515
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356515/suppl/GSM356515.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
|
GSM356516 | GPL339 |
|
Hunk WT rep6
|
Hunk WT, Mammary gland
|
Genotype: MMTV-c-myc/Hunk WT; Gender: Female; Tissue: Mammary gland tumors; Strain: FVB
|
Myc tumors with Hunk WT genotype
|
| Sample_geo_accession | GSM356516
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Dec 29 2008
| | Sample_last_update_date | Apr 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
| | Sample_growth_protocol_ch1 | A 129/Sv mouse genomic library (Stratagene, La Jolla, CA) was screened with a Hunk cDNA fragment (nt 1-706) (Gardner et al., 2000b). The Hunk gene was disrupted by replacement of a 1.1 kb fragment containing the putative promoter and exon 1 of Hunk with a pGKneo cassette flanked by LoxP sites. 25 µg of linearized vector was electroporated into 1x107 E14 ES cells (kindly provided by Marisa Bartolomei, University of Pennsylvania) and selected in 300 µg /ml G418. Properly target clones, as well as the resulting mice, were screened by Southern hybridization using a 3’ Xho I/Xmn I flanking probe. Lung protein lysates (7 mg) were subjected to immunoprecipitation and immunoblotting with Hunk-specific C-terminal antisera as previously described (Gardner et al., 2000a).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Affymetrix Generic Hybrdization
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneArray Scanner
| | Sample_data_processing | The data were analyzed with GCRMA using R and quantile normalized
| | Sample_platform_id | GPL339
| | Sample_contact_name | Lewis,A.,Chodosh
| | Sample_contact_email | chodosh@mail.med.upenn.edu
| | Sample_contact_fax | 215-573-6725
| | Sample_contact_laboratory | Lewis Chodosh
| | Sample_contact_department | Cancer Biology
| | Sample_contact_institute | University of Pennsylvania
| | Sample_contact_address | 421 curie blvd, BRB II/III 616
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356516/suppl/GSM356516.CEL.gz
| | Sample_series_id | GSE14226
| | Sample_data_row_count | 22690
| | |
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