Sample_geo_accession | GSM356831
|
Sample_status | Public on Dec 25 2009
|
Sample_submission_date | Dec 31 2008
|
Sample_last_update_date | Dec 31 2008
|
Sample_type | RNA
|
Sample_channel_count | 1
|
Sample_organism_ch1 | Mus musculus
|
Sample_taxid_ch1 | 10090
|
Sample_treatment_protocol_ch1 | Mice were bred under specific pathogen free (SPF) condiitons
|
Sample_growth_protocol_ch1 | IL-9 transgenic mice were described previously (Renauld et al., 1994. Oncogene 9:1327-32)
|
Sample_molecule_ch1 | total RNA
|
Sample_extract_protocol_ch1 | RNA was extracted using Tripure isolation reagent (Roche) according to the manufacturer’s instructions.
|
Sample_label_ch1 | biotin
|
Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse expression Array 430A. GeneChips were washed and stained in the Affymetrix genechip Fluidics Station 450.
|
Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
Sample_platform_id | GPL339
|
Sample_contact_name | Jean-Christophe,,Renauld
|
Sample_contact_email | renauld@bru.licr.org
|
Sample_contact_institute | Ludwig Institute for Cancer Research
|
Sample_contact_address | Avenue Hippocrate, 74
|
Sample_contact_city | Brussels
|
Sample_contact_zip/postal_code | B-1200
|
Sample_contact_country | Belgium
|
Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356831/suppl/GSM356831.CEL.gz
|
Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM356nnn/GSM356831/suppl/GSM356831.CHP.gz
|
Sample_series_id | GSE14249
|
Sample_data_row_count | 22690
|
|