Search results for the GEO ID: GSE14308
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Description
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GSM357839
GPL1261
Th2-1 CD4+ T cells cultured for 10 days CD4+ T cells cultured for 10 days Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357841
GPL1261
Th2-2 CD4+ T cells cultured in vitro for 10 days CD4+ T cells cultured in vitro for 10 days Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357842
GPL1261
Th1-2 CD4+ T cells cultured in vitro for 10 days CD4+ T cells cultured in vitro for 10 days Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357843
GPL1261
Th17-1 CD4+ T cells cultured in vitro for 10 days CD4+ T cells cultured in vitro for 10 days Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357844
GPL1261
Th1-1 CD4+ T cells cultured in vitro for 10 days CD4+ T cells cultured in vitro for 10 days Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357845
GPL1261
Th17-2 CD4+ T cells cultured in vitro for 10 days CD4+ T cells cultured in vitro for 10 days Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357847
GPL1261
Naive-1 Freshly isolated naive CD4+ T cells Freshly isolated naive CD4+ T cells Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357848
GPL1261
Naive-2 Naive CD4+ T cells Naive CD4+ T cells Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357849
GPL1261
iTreg-1 CD4+ T cells cultured in vitro for 10 days CD4+ T cells cultured in vitro for 10 days Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357850
GPL1261
iTreg-2 CD4+ T cells cultured in vitro for 10 days CD4+ T cells cultured in vitro for 10 days Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357852
GPL1261
nTreg-1 CD25hiCD4+ T cells CD25hiCD4+ T cells Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
GSM357853
GPL1261
nTreg-2 CD25hiCD4+ T cells CD25hiCD4+ T cells Total cellular RNA from either isolated naïve and natural Treg cells or cells cultured under Th1, Th2, Th17, and iTreg conditions was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Approximately 10 µg of RNA was labeled and hybridized to GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) according to the manufacturer's protocols. Expression values were determined using GeneChip Operating Software (GCOS) v1.1.1 software. All data analysis was performed using GeneSpring software GX 7.3.1 (Agilent Technologies).
 
 
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