Search results for the GEO ID: GSE14350 |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM358715 | GPL1261 |
|
C57BL/6 CD4+ CD25+ T effector cells #1
|
mouse spleen anti-CD3 48 hr
|
Genetic background: normal C57BL/6 mice
Age: 10 weeks
Number of mice per chip: 1
Tissue: spleen stimulated with anti-CD3
Purification: 48 hr after stimulation CD4+ T cells were enriched by MACS for CD4+ cells then sorted for CD4+ CD25hi cells
% CD4+ CD25+ post sort: 99.9%
|
B6 #1 EA21751
|
Sample_geo_accession | GSM358715
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Unfractionated spleen cells were cultured with anti-CD3 for 48 hr and then the activated CD4+ T cells were purified
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358715/suppl/GSM358715.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358740 | GPL1261 |
|
C57BL/6 CD4+ CD25+ T effector cells #2
|
mouse spleen anti-CD3 48 hr
|
Genetic background: normal C57BL/6 mice
Age: 14 weeks
Number of mice per chip: 1
Tissue: spleen stimulated with anti-CD3
Purification: 48 hr after stimulation, CD4+ T cells were enriched by MACS for CD4+ cells then sorted for CD4+ CD25hi cells
% CD4+ CD25+ post sort: 99.9%
|
B6 #2 EA21752
|
Sample_geo_accession | GSM358740
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Unfractionated spleen cells were cultured with anti-CD3 for 48 hr and then the activated CD4+ T cells were purified
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358740/suppl/GSM358740.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358741 | GPL1261 |
|
C57BL/6 CD4+ CD25+ T effector cells #3
|
mouse spleen anti-CD3 48 hr
|
Genetic background: normal C57BL/6 mice
Age: 14 weeks
Number of mice per chip: 1
Tissue: spleen stimulated with anti-CD3
Purification: 48 hr after stimulation, CD4+ T cell were enriched by MACS for CD4+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25+ post sort: 99.6%
|
B6 #1 EA21753
|
Sample_geo_accession | GSM358741
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Unfractionated spleen cells were cultured with anti-CD3 for 48 hr and then the activated CD4+ T cells were purified
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358741/suppl/GSM358741.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358742 | GPL1261 |
|
C57BL/6-2Rbeta-WT/thymus transgenic CD4+ CD25+ T effector cells #1
|
mouse spleen anti-CD3 48 hr
|
Genetic background: C57BL/6-2Rbeta-WT/thymus transgenic mice
Age: 15 weeks
Number of mice per chip: 1
Tissue: spleen stimulated with anti-CD3
Purification: 48 hr after stimulation, CD4+ T cell were enriched by MACS for CD4+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25+ post sort: 99.8%
|
C57BL/6-2Rbeta-WT/thymus transgenic #1 EA21754
|
Sample_geo_accession | GSM358742
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Unfractionated spleen cells were cultured with anti-CD3 for 48 hr and then the activated CD4+ T cells were purified
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358742/suppl/GSM358742.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358743 | GPL1261 |
|
C57BL/6-2Rbeta-WT/thymus transgenic CD4+ CD25+ T effector cells #2
|
mouse spleen anti-CD3 48 hr
|
Genetic background: C57BL/6-2Rbeta-WT/thymus transgenic mice
Age: 14 weeks
Number of mice per chip: 1
Tissue: spleen stimulated with anti-CD3
Purification: 48 hr after stimulation, CD4+ T cell were enriched by MACS for CD4+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25+ post sort: 99.5%
|
C57BL/6-2Rbeta-WT/thymus transgenic #1 EA21755
|
Sample_geo_accession | GSM358743
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Unfractionated spleen cells were cultured with anti-CD3 for 48 hr and then the activated CD4+ T cells were purified
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358743/suppl/GSM358743.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358744 | GPL1261 |
|
C57BL/6-2Rbeta-WT/thymus transgenic CD4+ CD25+ T effector cells #3
|
mouse spleen anti-CD3 48 hr
|
Genetic background: C57BL/6-2Rbeta-WT/thymus transgenic mice
Age: 9 weeks
Number of mice per chip: 1
Tissue: spleen stimulated with anti-CD3
Purification: Fresh spleen cells were depleted of CD8+ T cells and B cells, CD4+CD25+ T cells were enriched by MACS for CD25+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25+ post sort: 99.0%
|
C57BL/6-2Rbeta-WT/thymus transgenic EA21756
|
Sample_geo_accession | GSM358744
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Unfractionated spleen cells were cultured with anti-CD3 for 48 hr and then the activated CD4+ T cells were purified
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358744/suppl/GSM358744.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358746 | GPL1261 |
|
C57BL/6 CD4+ CD25+ Treg cells #1
|
mouse spleen
|
Genetic background: normal C57BL/6 mice
Age: 12 weeks
Number of mice per chip: 8
Tissue: spleen
Purification: Fresh spleen cells were depleted of CD8+ T cells and B cells, CD4+CD25+ T cells were enriched by MACS for CD25+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25hi post sort: 99.4%
|
B6 EA33255
|
Sample_geo_accession | GSM358746
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358746/suppl/GSM358746.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358748 | GPL1261 |
|
C57BL/6 CD4+ CD25+ Treg cells #2
|
mouse spleen
|
Genetic background: normal C57BL/6 mice
Age: 12 weeks
Number of mice per chip: 8
Tissue: spleen
Purification: Fresh spleen cells were depleted of CD8+ T cells and B cells, CD4+CD25+ T cells were enriched by MACS for CD25+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25hi post sort: 99.9%
|
B6 EA33257
|
Sample_geo_accession | GSM358748
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358748/suppl/GSM358748.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358749 | GPL1261 |
|
C57BL/6 CD4+ CD25+ Treg cells #3
|
mouse spleen
|
Genetic background: normal C57BL/6 mice
Age: 10 weeks
Number of mice per chip: 15
Tissue: spleen
Purification: Fresh spleen cells were depleted of CD8+ T cells and B cells, CD4+CD25+ T cells were enriched by MACS for CD25+ T cells then sorted for CD4+ CD25hi cells
|
B6 EA35092
|
Sample_geo_accession | GSM358749
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358749/suppl/GSM358749.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358753 | GPL1261 |
|
C57BL/6-2Rbeta-WT/thymus transgenic CD4+ CD25+ Treg cells #1
|
mouse spleen
|
Genetic background: C57BL/6-2Rbeta-WT/thymus transgenic mice
Age: 33 weeks
Number of mice per chip: 4
Tissue: spleen
Purification: Fresh spleen cells were depleted of CD8+ T cells and B cells, CD4+CD25+ T cells were enriched by MACS for CD25+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25hi post sort: 99.8%
|
C57BL/6-2Rbeta-WT/thymus transgenic EA33256
|
Sample_geo_accession | GSM358753
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358753/suppl/GSM358753.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358754 | GPL1261 |
|
C57BL/6-2Rbeta-WT/thymus transgenic CD4+ CD25+ Treg cells #2
|
mouse spleen
|
Genetic background: C57BL/6-2Rbeta-WT/thymus transgenic mice
Age: 13 weeks
Number of mice per chip: 8
Tissue: spleen
Purification: Fresh spleen cells were depleted of CD8+ T cells and B cells, CD4+CD25+ T cells were enriched by MACS for CD25+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25hi post sort: 99.8%
|
C57BL/6-2Rbeta-WT/thymus transgenic EA33258
|
Sample_geo_accession | GSM358754
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358754/suppl/GSM358754.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
GSM358755 | GPL1261 |
|
C57BL/6-2Rbeta-WT/thymus transgenic CD4+ CD25+ Treg cells #3
|
mouse spleen
|
Genetic background: C57BL/6-2Rbeta-WT/thymus transgenic mice
Age: 7-16 weeks
Number of mice per chip: 3
Tissue: spleen
Purification: Fresh spleen cells were depleted of CD8+ T cells and B cells, CD4+CD25+ T cells were enriched by MACS for CD25+ T cells then sorted for CD4+ CD25hi cells
% CD4+ CD25hi post sort: 99.6%
|
C57BL/6-2Rbeta-WT/thymus transgenic EA34569
|
Sample_geo_accession | GSM358755
| Sample_status | Public on Feb 22 2009
| Sample_submission_date | Jan 08 2009
| Sample_last_update_date | Feb 02 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA isolated with Trizol reagent and purified with RNAeassy minikit (Qiagen) and amplified 1X IVT as per Affymetrix technical manual.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_hyb_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_scan_protocol | per Affymetrix technical manual at Expression Analysis (Durhan, NC)
| Sample_data_processing | GC-RMA normalized through Geneshifter as log2 transformed values
| Sample_platform_id | GPL1261
| Sample_contact_name | Thomas,R,Malek
| Sample_contact_email | tmalek@med.miami.edu
| Sample_contact_department | Microbiology/Immunology
| Sample_contact_institute | University of Miami
| Sample_contact_address | 1600 NW 10th Avenue
| Sample_contact_city | Miami
| Sample_contact_state | FL
| Sample_contact_zip/postal_code | 33136
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM358nnn/GSM358755/suppl/GSM358755.CEL.gz
| Sample_series_id | GSE14350
| Sample_data_row_count | 45101
| |
|
|
|
Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|