Search results for the GEO ID: GSE14359  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM359137 | GPL96 |
|
HOBc Replicate 1; Non-neoplastic primary osteoblast cells
|
non-neoplastic human primary osteoblasts
|
Non-neoplastic primary osteoblast cells with limited live span in vitro
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359137
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Provitro GmbH, Berlin, Germany
| | Sample_treatment_protocol_ch1 | cells were grown to 70-80% confluence before RNA extraction
| | Sample_growth_protocol_ch1 | HOBc was maintained in special osteoblast growth medium (PromoCell, Heidelberg, Germany) with 10% FCS and 1% penicillin/streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359137/suppl/GSM359137.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359138 | GPL96 |
|
HOBc Replicate 2; Non-neoplastic primary osteoblast cells
|
Non-neoplastic primary human osteoblasts
|
Non-neoplastic primary osteoblast cells with limited live span in vitro
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359138
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Provitro GmbH, Berlin, Germany
| | Sample_treatment_protocol_ch1 | cells were grown to 70-80% confluence before RNA extraction
| | Sample_growth_protocol_ch1 | HOBc was maintained in special osteoblast growth medium (PromoCell, Heidelberg, Germany) with 10% FCS and 1% penicillin/streptomycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359138/suppl/GSM359138.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359139 | GPL96 |
|
OS1 Replicate 1; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, female, 15 years, femur, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359139
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359139/suppl/GSM359139.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359140 | GPL96 |
|
OS1 Replicate 2; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, female, 15 years, femur, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359140
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359140/suppl/GSM359140.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359141 | GPL96 |
|
OS11 Replicate 1; osteosarcoma lung metastasis tissue
|
human osteosarcoma tissue
|
osteosarcoma lung metastasis tissue, female, 45 years, lung, grade 1
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359141
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359141/suppl/GSM359141.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359142 | GPL96 |
|
OS11 Replicate 2; osteosarcoma lung metastasis tissue
|
human osteosarcoma tissue
|
osteosarcoma lung metastasis tissue, female, 45 years, lung, grade 1
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359142
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359142/suppl/GSM359142.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359143 | GPL96 |
|
OS15 Replicate 1; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, female, 74 years, femur, grade 2
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359143
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359143/suppl/GSM359143.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359144 | GPL96 |
|
OS15 Replicate 2; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, female, 74 years, femur, grade 2
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359144
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359144/suppl/GSM359144.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359145 | GPL96 |
|
OS16 Replicate 1; osteosarcoma lung metastasis tissue
|
human osteosarcoma tissue
|
osteosarcoma lung metastasis tissue, female, 37 years, lung, grade 2
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359145
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359145/suppl/GSM359145.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359146 | GPL96 |
|
OS16 Replicate 2; osteosarcoma lung metastasis tissue
|
human osteosarcoma tissue
|
osteosarcoma lung metastasis tissue, female, 37 years, lung, grade 2
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359146
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359146/suppl/GSM359146.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359147 | GPL96 |
|
OS18 Replicate 1; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, male, 7 years, femur, grade 2
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359147
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359147/suppl/GSM359147.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359148 | GPL96 |
|
OS18 Replicate 2; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, male, 7 years, femur, grade 2
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359148
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359148/suppl/GSM359148.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359149 | GPL96 |
|
OS24 Replicate 1; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, male, 17 years, femur, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359149
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359149/suppl/GSM359149.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359150 | GPL96 |
|
OS24 Replicate 2; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, male, 17 years, femur, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359150
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359150/suppl/GSM359150.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359151 | GPL96 |
|
OS4 Replicate 1; osteosarcoma lung metastasis tissue
|
human osteosarcoma tissue
|
osteosarcoma lung metastasis tissue, male, 40 years, lung, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359151
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359151/suppl/GSM359151.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359152 | GPL96 |
|
OS4 Replicate 2; osteosarcoma lung metastasis tissue
|
human osteosarcoma tissue
|
osteosarcoma lung metastasis tissue, male, 40 years, lung, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359152
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359152/suppl/GSM359152.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359153 | GPL96 |
|
OS6 Replicate 1; osteosarcoma lung metastasis tissue
|
human osteosarcoma tissue
|
osteosarcoma lung metastasis tissue, female, 21 years, lung, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359153
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359153/suppl/GSM359153.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359154 | GPL96 |
|
OS6 Replicate 2; osteosarcoma lung metastasis tissue
|
human osteosarcoma tissue
|
osteosarcoma lung metastasis tissue, female, 21 years, lung, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359154
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359154/suppl/GSM359154.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359155 | GPL96 |
|
OS9 Replicate 1; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, male, 23 years, tibia, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359155
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359155/suppl/GSM359155.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
GSM359156 | GPL96 |
|
OS9 Replicate 2; conventional osteosarcoma tissue
|
human osteosarcoma tissue
|
conventional osteosarcoma tissue, male, 23 years, tibia, grade 3
|
Scanning was carried out by Agilent G2500A GeneArrayScanner (Konfocal argon laser). Software of Affymetrix MAS (MicroArraySuite) 5.0 and DATA-mining-tool 2.0 and Silicongenetics GeneSpring 6.0 were used.
|
| Sample_geo_accession | GSM359156
| | Sample_status | Public on Jun 30 2010
| | Sample_submission_date | Jan 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Charite Universitätsmedizin Berlin
| | Sample_treatment_protocol_ch1 | The tissue specimens were fixed in 4% neutral buffered formaldehyde, bone containing tisuue was EDTA-decalcified and embedded in paraffin. Each slide was stained with H&E for Pathologist evaluation to determine tumor content. The diagnosis of the tumor was confirmed according to WHO criteria.
| | Sample_growth_protocol_ch1 | Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Osteosarcoma tissue samples were collected in liquid nitrogen and stored at -80°C until use.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Tri-Reagent (Sigma) as well as the RNeasy-mini-kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | According to the standard Affymetrix protocol
| | Sample_hyb_protocol | According to the standard Affymetrix protocol
| | Sample_scan_protocol | According to the standard Affymetrix protocol
| | Sample_data_processing | According to Gcos1.4 protocol
| | Sample_platform_id | GPL96
| | Sample_contact_name | Raphaela,,Fritsche
| | Sample_contact_email | raphaela.fritsche@charite.de
| | Sample_contact_laboratory | Systems Biology of Molecular Networks
| | Sample_contact_department | Institute of Pathology
| | Sample_contact_institute | Charitè Universitätsklinikum Charite
| | Sample_contact_address | Chariteplatz 1
| | Sample_contact_city | Berlin
| | Sample_contact_zip/postal_code | 10117
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359156/suppl/GSM359156.CEL.gz
| | Sample_series_id | GSE14359
| | Sample_data_row_count | 22283
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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