Search results for the GEO ID: GSE14365 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM359245 | GPL1261 |
|
Aireko_1
|
Aire-deficient mTEC
|
Strain: C57BL/6
|
Gene expression data from Aire-deficient mTECs, first biological replicate
|
Sample_geo_accession | GSM359245
| Sample_status | Public on Mar 15 2009
| Sample_submission_date | Jan 09 2009
| Sample_last_update_date | Jan 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | none
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction was performed using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 100ng of total RNA was amplified using T7-oligo dT and the Megascript T7 kit (Ambion). A second round of cDNA synthesis was performed using first round amplified RNA.
| Sample_label_protocol_ch1 | Amplified RNA was biotin-labelled using the GeneChip IVT Labelling Kit.
| Sample_hyb_protocol | 15 micro-grams of labelled RNA were then fragmented as described in the Affymetrix manual. The mouse genome 430 2.0 arrays were hybridised overnight and washed.
| Sample_scan_protocol | Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
| Sample_data_processing | The intensities for each probe set were normalized and summarized using the Robust Multi-array Analysis (RMA) algorithm in the software programme R.
| Sample_platform_id | GPL1261
| Sample_contact_name | Francois-Xavier,,Hubert
| Sample_contact_email | hubert@wehi.edu.au
| Sample_contact_department | Immunology
| Sample_contact_institute | The Walter and Eliza Hall Institute of Medical Research
| Sample_contact_address | 1G Royal Parade
| Sample_contact_city | Melbourne
| Sample_contact_state | Victoria
| Sample_contact_zip/postal_code | 3052
| Sample_contact_country | Australia
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359245/suppl/GSM359245.CEL.gz
| Sample_series_id | GSE14365
| Sample_data_row_count | 45101
| |
|
GSM359246 | GPL1261 |
|
wt_1
|
Wild type mTEC
|
Strain: C57BL/6
|
Gene expression data from wild type mTECs, first biological replicate
|
Sample_geo_accession | GSM359246
| Sample_status | Public on Mar 15 2009
| Sample_submission_date | Jan 09 2009
| Sample_last_update_date | Jan 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | none
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction was performed using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 100ng of total RNA was amplified using T7-oligo dT and the Megascript T7 kit (Ambion). A second round of cDNA synthesis was performed using first round amplified RNA.
| Sample_label_protocol_ch1 | Amplified RNA was biotin-labelled using the GeneChip IVT Labelling Kit.
| Sample_hyb_protocol | 15 micro-grams of labelled RNA were then fragmented as described in the Affymetrix manual. The mouse genome 430 2.0 arrays were hybridised overnight and washed.
| Sample_scan_protocol | Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
| Sample_data_processing | The intensities for each probe set were normalized and summarized using the Robust Multi-array Analysis (RMA) algorithm in the software programme R.
| Sample_platform_id | GPL1261
| Sample_contact_name | Francois-Xavier,,Hubert
| Sample_contact_email | hubert@wehi.edu.au
| Sample_contact_department | Immunology
| Sample_contact_institute | The Walter and Eliza Hall Institute of Medical Research
| Sample_contact_address | 1G Royal Parade
| Sample_contact_city | Melbourne
| Sample_contact_state | Victoria
| Sample_contact_zip/postal_code | 3052
| Sample_contact_country | Australia
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359246/suppl/GSM359246.CEL.gz
| Sample_series_id | GSE14365
| Sample_data_row_count | 45101
| |
|
GSM359247 | GPL1261 |
|
wt_2
|
Wild type mTEC
|
Strain: C57BL/6
|
Gene expression data from wild type mTECs, second biological replicate
|
Sample_geo_accession | GSM359247
| Sample_status | Public on Mar 15 2009
| Sample_submission_date | Jan 09 2009
| Sample_last_update_date | Jan 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | none
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction was performed using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 100ng of total RNA was amplified using T7-oligo dT and the Megascript T7 kit (Ambion). A second round of cDNA synthesis was performed using first round amplified RNA.
| Sample_label_protocol_ch1 | Amplified RNA was biotin-labelled using the GeneChip IVT Labelling Kit.
| Sample_hyb_protocol | 15 micro-grams of labelled RNA were then fragmented as described in the Affymetrix manual. The mouse genome 430 2.0 arrays were hybridised overnight and washed.
| Sample_scan_protocol | Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
| Sample_data_processing | The intensities for each probe set were normalized and summarized using the Robust Multi-array Analysis (RMA) algorithm in the software programme R.
| Sample_platform_id | GPL1261
| Sample_contact_name | Francois-Xavier,,Hubert
| Sample_contact_email | hubert@wehi.edu.au
| Sample_contact_department | Immunology
| Sample_contact_institute | The Walter and Eliza Hall Institute of Medical Research
| Sample_contact_address | 1G Royal Parade
| Sample_contact_city | Melbourne
| Sample_contact_state | Victoria
| Sample_contact_zip/postal_code | 3052
| Sample_contact_country | Australia
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359247/suppl/GSM359247.CEL.gz
| Sample_series_id | GSE14365
| Sample_data_row_count | 45101
| |
|
GSM359248 | GPL1261 |
|
Aireko_2
|
Aire-deficient mTEC
|
Strain: C57BL/6
|
Gene expression data from Aire-deficient mTECs, second biological replicate
|
Sample_geo_accession | GSM359248
| Sample_status | Public on Mar 15 2009
| Sample_submission_date | Jan 09 2009
| Sample_last_update_date | Jan 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | none
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction was performed using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 100ng of total RNA was amplified using T7-oligo dT and the Megascript T7 kit (Ambion). A second round of cDNA synthesis was performed using first round amplified RNA.
| Sample_label_protocol_ch1 | Amplified RNA was biotin-labelled using the GeneChip IVT Labelling Kit.
| Sample_hyb_protocol | 15 micro-grams of labelled RNA were then fragmented as described in the Affymetrix manual. The mouse genome 430 2.0 arrays were hybridised overnight and washed.
| Sample_scan_protocol | Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
| Sample_data_processing | The intensities for each probe set were normalized and summarized using the Robust Multi-array Analysis (RMA) algorithm in the software programme R.
| Sample_platform_id | GPL1261
| Sample_contact_name | Francois-Xavier,,Hubert
| Sample_contact_email | hubert@wehi.edu.au
| Sample_contact_department | Immunology
| Sample_contact_institute | The Walter and Eliza Hall Institute of Medical Research
| Sample_contact_address | 1G Royal Parade
| Sample_contact_city | Melbourne
| Sample_contact_state | Victoria
| Sample_contact_zip/postal_code | 3052
| Sample_contact_country | Australia
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359248/suppl/GSM359248.CEL.gz
| Sample_series_id | GSE14365
| Sample_data_row_count | 45101
| |
|
GSM359249 | GPL1261 |
|
wt_3
|
Wild type mTEC
|
Strain: C57BL/6
|
Gene expression data from wild type mTECs, third biological replicate
|
Sample_geo_accession | GSM359249
| Sample_status | Public on Mar 15 2009
| Sample_submission_date | Jan 09 2009
| Sample_last_update_date | Jan 09 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | none
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA extraction was performed using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 100ng of total RNA was amplified using T7-oligo dT and the Megascript T7 kit (Ambion). A second round of cDNA synthesis was performed using first round amplified RNA.
| Sample_label_protocol_ch1 | Amplified RNA was biotin-labelled using the GeneChip IVT Labelling Kit.
| Sample_hyb_protocol | 15 micro-grams of labelled RNA were then fragmented as described in the Affymetrix manual. The mouse genome 430 2.0 arrays were hybridised overnight and washed.
| Sample_scan_protocol | Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
| Sample_data_processing | The intensities for each probe set were normalized and summarized using the Robust Multi-array Analysis (RMA) algorithm in the software programme R.
| Sample_platform_id | GPL1261
| Sample_contact_name | Francois-Xavier,,Hubert
| Sample_contact_email | hubert@wehi.edu.au
| Sample_contact_department | Immunology
| Sample_contact_institute | The Walter and Eliza Hall Institute of Medical Research
| Sample_contact_address | 1G Royal Parade
| Sample_contact_city | Melbourne
| Sample_contact_state | Victoria
| Sample_contact_zip/postal_code | 3052
| Sample_contact_country | Australia
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359249/suppl/GSM359249.CEL.gz
| Sample_series_id | GSE14365
| Sample_data_row_count | 45101
| |
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