Search results for the GEO ID: GSE14386 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM359574 | GPL570 |
|
CIS patient No.1_Untreated
|
PBMCs from No.1 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359574
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359574/suppl/GSM359574.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359575 | GPL570 |
|
CIS patient No.2_Untreated
|
PBMCs from No.2 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359575
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359575/suppl/GSM359575.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359576 | GPL570 |
|
CIS patient No.3_Untreated
|
PBMCs from No.3 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359576
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359576/suppl/GSM359576.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359577 | GPL570 |
|
CIS patient No.4_Untreated
|
PBMCs from No.4 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359577
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359577/suppl/GSM359577.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359578 | GPL570 |
|
CIS patient No.5_Untreated
|
PBMCs from No.5 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359578
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359578/suppl/GSM359578.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359579 | GPL570 |
|
CIS patient No.6_Untreated
|
PBMCs from No.6 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359579
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359579/suppl/GSM359579.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359580 | GPL570 |
|
CIS patient No.7_Untreated
|
PBMCs from No.7 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359580
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359580/suppl/GSM359580.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359581 | GPL570 |
|
CIS patient No.8_Untreated
|
PBMCs from No.8 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359581
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359581/suppl/GSM359581.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359582 | GPL570 |
|
CIS patient No.9_Untreated
|
PBMCs from No.9 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359582
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359582/suppl/GSM359582.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359583 | GPL570 |
|
CIS patient No.10_Untreated
|
PBMCs from No.10 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359583
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359583/suppl/GSM359583.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359584 | GPL570 |
|
CIS patient No.11_Untreated
|
PBMCs from No.11 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359584
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359584/suppl/GSM359584.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359585 | GPL570 |
|
CIS patient No.12_Untreated
|
PBMCs from No.12 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359585
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359585/suppl/GSM359585.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359586 | GPL570 |
|
CIS patient No.13_Untreated
|
PBMCs from No.13 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359586
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359586/suppl/GSM359586.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359587 | GPL570 |
|
CIS patient No.14_Untreated
|
PBMCs from No.14 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359587
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359587/suppl/GSM359587.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359588 | GPL570 |
|
CIS patient No.15_Untreated
|
PBMCs from No.15 CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in absence of IFNb-1a
|
Sample_geo_accession | GSM359588
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359588/suppl/GSM359588.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359589 | GPL570 |
|
CIS patient No.1_Treated
|
PBMCs from No.1 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359589
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359589/suppl/GSM359589.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359590 | GPL570 |
|
CIS patient No.2_Treated
|
PBMCs from No.2 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359590
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359590/suppl/GSM359590.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359591 | GPL570 |
|
CIS patient No.3_Treated
|
PBMCs from No.3 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359591
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359591/suppl/GSM359591.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359592 | GPL570 |
|
CIS patient No.4_Treated
|
PBMCs from No.4 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359592
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359592/suppl/GSM359592.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359593 | GPL570 |
|
CIS patient No.5_Treated
|
PBMCs from No.5 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359593
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359593/suppl/GSM359593.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359594 | GPL570 |
|
CIS patient No.6_Treated
|
PBMCs from No.6 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359594
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359594/suppl/GSM359594.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359595 | GPL570 |
|
CIS patient No.7_Treated
|
PBMCs from No.7 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359595
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359595/suppl/GSM359595.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359596 | GPL570 |
|
CIS patient No.8_Treated
|
PBMCs from No.8 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359596
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359596/suppl/GSM359596.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359597 | GPL570 |
|
CIS patient No.9_Treated
|
PBMCs from No.9 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359597
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359597/suppl/GSM359597.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359598 | GPL570 |
|
CIS patient No.10_Treated
|
PBMCs from No.10 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359598
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359598/suppl/GSM359598.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359599 | GPL570 |
|
CIS patient No.11_Treated
|
PBMCs from No.11 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359599
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359599/suppl/GSM359599.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359600 | GPL570 |
|
CIS patient No.12_Treated
|
PBMCs from No.12 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359600
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359600/suppl/GSM359600.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359601 | GPL570 |
|
CIS patient No.13_Treated
|
PBMCs from No.13 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359601
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359601/suppl/GSM359601.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359602 | GPL570 |
|
CIS patient No.14_Treated
|
PBMCs from No.14 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359602
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359602/suppl/GSM359602.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
GSM359603 | GPL570 |
|
CIS patient No.15_Treated
|
PBMCs from No.15 CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
107 PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized αCD3 (1 μg/ml) and αCD28 (5 μg/ml) mAb (BD Biosciences) in the absence or presence of IFNβ-1a (1000 U/ml) (EMD Serono Inc) for 24 h in serum-free medium (Gibco).
|
Gene expression of PBMCs from CIS patient stimulated with aCD3+aCD28 mAb for 24h in presence of IFNb-1a
|
Sample_geo_accession | GSM359603
| Sample_status | Public on Jan 13 2009
| Sample_submission_date | Jan 12 2009
| Sample_last_update_date | Jan 12 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | 10 million PBMCs per condition derived from 15 CIS patients were stimulated with plate-immobilized aCD3 and aCD28 mAb in the absence or presence of IFNb-1a for 24 h in serum-free medium.
| Sample_growth_protocol_ch1 | PBMCs were seperated from 15 CIS patients and cultured in serum free medium.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
| Sample_hyb_protocol | 15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
| Sample_scan_protocol | The arrays were scanned with the Hewlett Packard GeneArray Scanner.
| Sample_data_processing | Affymetrix GeneChip® Microarray Suite 5.0 software
| Sample_platform_id | GPL570
| Sample_contact_name | Silva,,Markovic-Plese
| Sample_contact_email | markovics@neurology.unc.edu
| Sample_contact_phone | 919 966 3701
| Sample_contact_institute | University of North Carolina at Chapel Hill
| Sample_contact_address | 105 Mason Farm Rd
| Sample_contact_city | Chapel Hill
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27516
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359603/suppl/GSM359603.CEL.gz
| Sample_series_id | GSE14386
| Sample_data_row_count | 54675
| |
|
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
|
Select GSMs and click on "Add groups" |
Enter the group name here: |
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