Result

Search results for the GEO ID: GSE14402

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM359894

GPL1355

DMSO-treated chondrocytes, biological replicate 1

Primary chondrocytes derived from the femoral condyles of neonatal rats, pre-treated with DMSO for 30 min followed by vehicle (BSA) for 24h.

Strain:Sprague Dawley, Age:neonatal, Tissue:rat femoral condyle, Cell:chondrocytes, Contidion: Cell Culture/In vitro

none

Sample_geo_accession	GSM359894
Sample_status	Public on Jan 14 2009
Sample_submission_date	Jan 13 2009
Sample_last_update_date	Jan 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Chondrocytes were incubated in serum free media overnight. The next day, chondrocytes were treated with DMSO or U0126 for 30 minutes prior to addition of vehicle (BSA) or TNF-a (30 ng/ml) for 24 h.
Sample_growth_protocol_ch1	Chondrocytes were isolated from the femoral condyles of neonatal (one day old) rats and plated onto tissue culture plastic at a density of ~2.5 x 104 cells/cm2. Cells were grown in culture until confluence was reached, typcially 6-7 days. Media used was RPMI-1640 supplimented with 5% fetal bovine serum (FBS), 100 U/ml Penicillin, 100 g/ml streptomycin and 1% HEPES buffer. The media was changed every 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was collected by Trizol followed by Qiagen Rneasy mini clean-up, as per manufacturers' instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared as per standard Affymetrix protocol using 10mg of total RNA to start (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_hyb_protocol	Following cRNA fragmentation, cRNA were hybridized to GeneChip RAT230 2.0 arrays and stained using the Affymetrix Fluidics 450 station as per manufacturers instructions (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G
Sample_data_processing	Data was analyzed using Genespring GX 7.3 (Agilent). Values were transformed using RMA. Raw expression values <0.01 were set to 0.01 and the normalization per chip was set to the 50th percentile. Genes were normalized to DMSO control within each biological replicate.
Sample_platform_id	GPL1355
Sample_contact_name	Jason,Scott,Rockel
Sample_contact_email	jsrockel@uwo.ca
Sample_contact_phone	519-661-2111
Sample_contact_laboratory	Dr. Andrew Leask
Sample_contact_department	Anatomy and Cell Biology
Sample_contact_institute	CIHR Group in Skeletal Development and Remodeling
Sample_contact_address	The University of Western Ontario
Sample_contact_city	London
Sample_contact_state	Ontario
Sample_contact_zip/postal_code	N6A 5C1
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359894/suppl/GSM359894.CEL.gz
Sample_series_id	GSE14402
Sample_data_row_count	31099

GSM359895

GPL1355

DMSO-treated chondrocytes, biological replicate 2

Primary chondrocytes derived from the femoral condyles of neonatal rats, pre-treated with DMSO for 30 min followed by vehicle (BSA) for 24h.

Strain:Sprague Dawley, Age:neonatal, Tissue:rat femoral condyle, Cell:chondrocytes, Contidion: Cell Culture/In vitro

none

Sample_geo_accession	GSM359895
Sample_status	Public on Jan 14 2009
Sample_submission_date	Jan 13 2009
Sample_last_update_date	Jan 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Chondrocytes were incubated in serum free media overnight. The next day, chondrocytes were treated with DMSO or U0126 for 30 minutes prior to addition of vehicle (BSA) or TNF-a (30 ng/ml) for 24 h.
Sample_growth_protocol_ch1	Chondrocytes were isolated from the femoral condyles of neonatal (one day old) rats and plated onto tissue culture plastic at a density of ~2.5 x 104 cells/cm2. Cells were grown in culture until confluence was reached, typcially 6-7 days. Media used was RPMI-1640 supplimented with 5% fetal bovine serum (FBS), 100 U/ml Penicillin, 100 g/ml streptomycin and 1% HEPES buffer. The media was changed every 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was collected by Trizol followed by Qiagen Rneasy mini clean-up, as per manufacturers' instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared as per standard Affymetrix protocol using 10mg of total RNA to start (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_hyb_protocol	Following cRNA fragmentation, cRNA were hybridized to GeneChip RAT230 2.0 arrays and stained using the Affymetrix Fluidics 450 station as per manufacturers instructions (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G
Sample_data_processing	Data was analyzed using Genespring GX 7.3 (Agilent). Values were transformed using RMA. Raw expression values <0.01 were set to 0.01 and the normalization per chip was set to the 50th percentile. Genes were normalized to DMSO control within each biological replicate.
Sample_platform_id	GPL1355
Sample_contact_name	Jason,Scott,Rockel
Sample_contact_email	jsrockel@uwo.ca
Sample_contact_phone	519-661-2111
Sample_contact_laboratory	Dr. Andrew Leask
Sample_contact_department	Anatomy and Cell Biology
Sample_contact_institute	CIHR Group in Skeletal Development and Remodeling
Sample_contact_address	The University of Western Ontario
Sample_contact_city	London
Sample_contact_state	Ontario
Sample_contact_zip/postal_code	N6A 5C1
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359895/suppl/GSM359895.CEL.gz
Sample_series_id	GSE14402
Sample_data_row_count	31099

GSM359896

GPL1355

U0126-treated chondrocytes, biological replicate 1

Primary chondrocytes derived from the femoral condyles of neonatal rats, pre-treated with U0126 for 30 min followed by vehicle (BSA) for 24h.

Strain:Sprague Dawley, Age:neonatal, Tissue:rat femoral condyle, Cell:chondrocytes, Contidion: Cell Culture/In vitro

none

Sample_geo_accession	GSM359896
Sample_status	Public on Jan 14 2009
Sample_submission_date	Jan 13 2009
Sample_last_update_date	Jan 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Chondrocytes were incubated in serum free media overnight. The next day, chondrocytes were treated with DMSO or U0126 for 30 minutes prior to addition of vehicle (BSA) or TNF-a (30 ng/ml) for 24 h.
Sample_growth_protocol_ch1	Chondrocytes were isolated from the femoral condyles of neonatal (one day old) rats and plated onto tissue culture plastic at a density of ~2.5 x 104 cells/cm2. Cells were grown in culture until confluence was reached, typcially 6-7 days. Media used was RPMI-1640 supplimented with 5% fetal bovine serum (FBS), 100 U/ml Penicillin, 100 g/ml streptomycin and 1% HEPES buffer. The media was changed every 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was collected by Trizol followed by Qiagen Rneasy mini clean-up, as per manufacturers' instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared as per standard Affymetrix protocol using 10mg of total RNA to start (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_hyb_protocol	Following cRNA fragmentation, cRNA were hybridized to GeneChip RAT230 2.0 arrays and stained using the Affymetrix Fluidics 450 station as per manufacturers instructions (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G
Sample_data_processing	Data was analyzed using Genespring GX 7.3 (Agilent). Values were transformed using RMA. Raw expression values <0.01 were set to 0.01 and the normalization per chip was set to the 50th percentile. Genes were normalized to DMSO control within each biological replicate.
Sample_platform_id	GPL1355
Sample_contact_name	Jason,Scott,Rockel
Sample_contact_email	jsrockel@uwo.ca
Sample_contact_phone	519-661-2111
Sample_contact_laboratory	Dr. Andrew Leask
Sample_contact_department	Anatomy and Cell Biology
Sample_contact_institute	CIHR Group in Skeletal Development and Remodeling
Sample_contact_address	The University of Western Ontario
Sample_contact_city	London
Sample_contact_state	Ontario
Sample_contact_zip/postal_code	N6A 5C1
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359896/suppl/GSM359896.CEL.gz
Sample_series_id	GSE14402
Sample_data_row_count	31099

GSM359897

GPL1355

U0126-treated chondrocytes, biological replicate 2

Primary chondrocytes derived from the femoral condyles of neonatal rats, pre-treated with U0126 for 30 min followed by vehicle (BSA) for 24h.

Strain:Sprague Dawley, Age:neonatal, Tissue:rat femoral condyle, Cell:chondrocytes, Contidion: Cell Culture/In vitro

none

Sample_geo_accession	GSM359897
Sample_status	Public on Jan 14 2009
Sample_submission_date	Jan 13 2009
Sample_last_update_date	Jan 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Chondrocytes were incubated in serum free media overnight. The next day, chondrocytes were treated with DMSO or U0126 for 30 minutes prior to addition of vehicle (BSA) or TNF-a (30 ng/ml) for 24 h.
Sample_growth_protocol_ch1	Chondrocytes were isolated from the femoral condyles of neonatal (one day old) rats and plated onto tissue culture plastic at a density of ~2.5 x 104 cells/cm2. Cells were grown in culture until confluence was reached, typcially 6-7 days. Media used was RPMI-1640 supplimented with 5% fetal bovine serum (FBS), 100 U/ml Penicillin, 100 g/ml streptomycin and 1% HEPES buffer. The media was changed every 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was collected by Trizol followed by Qiagen Rneasy mini clean-up, as per manufacturers' instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared as per standard Affymetrix protocol using 10mg of total RNA to start (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_hyb_protocol	Following cRNA fragmentation, cRNA were hybridized to GeneChip RAT230 2.0 arrays and stained using the Affymetrix Fluidics 450 station as per manufacturers instructions (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G
Sample_data_processing	Data was analyzed using Genespring GX 7.3 (Agilent). Values were transformed using RMA. Raw expression values <0.01 were set to 0.01 and the normalization per chip was set to the 50th percentile. Genes were normalized to DMSO control within each biological replicate.
Sample_platform_id	GPL1355
Sample_contact_name	Jason,Scott,Rockel
Sample_contact_email	jsrockel@uwo.ca
Sample_contact_phone	519-661-2111
Sample_contact_laboratory	Dr. Andrew Leask
Sample_contact_department	Anatomy and Cell Biology
Sample_contact_institute	CIHR Group in Skeletal Development and Remodeling
Sample_contact_address	The University of Western Ontario
Sample_contact_city	London
Sample_contact_state	Ontario
Sample_contact_zip/postal_code	N6A 5C1
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359897/suppl/GSM359897.CEL.gz
Sample_series_id	GSE14402
Sample_data_row_count	31099

GSM359898

GPL1355

TNF-a treated chondrocytes, biological replicate 1

Primary chondrocytes derived from the femoral condyles of neonatal rats, pre-treated with DMSO for 30 min followed by TNF-a for 24h.

Strain:Sprague Dawley, Age:neonatal, Tissue:rat femoral condyle, Cell:chondrocytes, Contidion: Cell Culture/In vitro

none

Sample_geo_accession	GSM359898
Sample_status	Public on Jan 14 2009
Sample_submission_date	Jan 13 2009
Sample_last_update_date	Jan 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Chondrocytes were incubated in serum free media overnight. The next day, chondrocytes were treated with DMSO or U0126 for 30 minutes prior to addition of vehicle (BSA) or TNF-a (30 ng/ml) for 24 h.
Sample_growth_protocol_ch1	Chondrocytes were isolated from the femoral condyles of neonatal (one day old) rats and plated onto tissue culture plastic at a density of ~2.5 x 104 cells/cm2. Cells were grown in culture until confluence was reached, typcially 6-7 days. Media used was RPMI-1640 supplimented with 5% fetal bovine serum (FBS), 100 U/ml Penicillin, 100 g/ml streptomycin and 1% HEPES buffer. The media was changed every 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was collected by Trizol followed by Qiagen Rneasy mini clean-up, as per manufacturers' instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared as per standard Affymetrix protocol using 10mg of total RNA to start (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_hyb_protocol	Following cRNA fragmentation, cRNA were hybridized to GeneChip RAT230 2.0 arrays and stained using the Affymetrix Fluidics 450 station as per manufacturers instructions (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G
Sample_data_processing	Data was analyzed using Genespring GX 7.3 (Agilent). Values were transformed using RMA. Raw expression values <0.01 were set to 0.01 and the normalization per chip was set to the 50th percentile. Genes were normalized to DMSO control within each biological replicate.
Sample_platform_id	GPL1355
Sample_contact_name	Jason,Scott,Rockel
Sample_contact_email	jsrockel@uwo.ca
Sample_contact_phone	519-661-2111
Sample_contact_laboratory	Dr. Andrew Leask
Sample_contact_department	Anatomy and Cell Biology
Sample_contact_institute	CIHR Group in Skeletal Development and Remodeling
Sample_contact_address	The University of Western Ontario
Sample_contact_city	London
Sample_contact_state	Ontario
Sample_contact_zip/postal_code	N6A 5C1
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359898/suppl/GSM359898.CEL.gz
Sample_series_id	GSE14402
Sample_data_row_count	31099

GSM359899

GPL1355

TNF-a treated chondrocytes, biological replicate 2

Primary chondrocytes derived from the femoral condyles of neonatal rats, pre-treated with DMSO for 30 min followed by TNF-a for 24h.

Strain:Sprague Dawley, Age:neonatal, Tissue:rat femoral condyle, Cell:chondrocytes, Contidion: Cell Culture/In vitro

none

Sample_geo_accession	GSM359899
Sample_status	Public on Jan 14 2009
Sample_submission_date	Jan 13 2009
Sample_last_update_date	Jan 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Chondrocytes were incubated in serum free media overnight. The next day, chondrocytes were treated with DMSO or U0126 for 30 minutes prior to addition of vehicle (BSA) or TNF-a (30 ng/ml) for 24 h.
Sample_growth_protocol_ch1	Chondrocytes were isolated from the femoral condyles of neonatal (one day old) rats and plated onto tissue culture plastic at a density of ~2.5 x 104 cells/cm2. Cells were grown in culture until confluence was reached, typcially 6-7 days. Media used was RPMI-1640 supplimented with 5% fetal bovine serum (FBS), 100 U/ml Penicillin, 100 g/ml streptomycin and 1% HEPES buffer. The media was changed every 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was collected by Trizol followed by Qiagen Rneasy mini clean-up, as per manufacturers' instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared as per standard Affymetrix protocol using 10mg of total RNA to start (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_hyb_protocol	Following cRNA fragmentation, cRNA were hybridized to GeneChip RAT230 2.0 arrays and stained using the Affymetrix Fluidics 450 station as per manufacturers instructions (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G
Sample_data_processing	Data was analyzed using Genespring GX 7.3 (Agilent). Values were transformed using RMA. Raw expression values <0.01 were set to 0.01 and the normalization per chip was set to the 50th percentile. Genes were normalized to DMSO control within each biological replicate.
Sample_platform_id	GPL1355
Sample_contact_name	Jason,Scott,Rockel
Sample_contact_email	jsrockel@uwo.ca
Sample_contact_phone	519-661-2111
Sample_contact_laboratory	Dr. Andrew Leask
Sample_contact_department	Anatomy and Cell Biology
Sample_contact_institute	CIHR Group in Skeletal Development and Remodeling
Sample_contact_address	The University of Western Ontario
Sample_contact_city	London
Sample_contact_state	Ontario
Sample_contact_zip/postal_code	N6A 5C1
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359899/suppl/GSM359899.CEL.gz
Sample_series_id	GSE14402
Sample_data_row_count	31099

GSM359900

GPL1355

U0126 and TNF-a-treated chondrocytes, biological replicate 1

Primary chondrocytes derived from the femoral condyles of neonatal rats, pre-treated with U0126 for 30 min followed by TNF-a for 24h.

Strain:Sprague Dawley, Age:neonatal, Tissue:rat femoral condyle, Cell:chondrocytes, Contidion: Cell Culture/In vitro

none

Sample_geo_accession	GSM359900
Sample_status	Public on Jan 14 2009
Sample_submission_date	Jan 13 2009
Sample_last_update_date	Jan 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Chondrocytes were incubated in serum free media overnight. The next day, chondrocytes were treated with DMSO or U0126 for 30 minutes prior to addition of vehicle (BSA) or TNF-a (30 ng/ml) for 24 h.
Sample_growth_protocol_ch1	Chondrocytes were isolated from the femoral condyles of neonatal (one day old) rats and plated onto tissue culture plastic at a density of ~2.5 x 104 cells/cm2. Cells were grown in culture until confluence was reached, typcially 6-7 days. Media used was RPMI-1640 supplimented with 5% fetal bovine serum (FBS), 100 U/ml Penicillin, 100 g/ml streptomycin and 1% HEPES buffer. The media was changed every 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was collected by Trizol followed by Qiagen Rneasy mini clean-up, as per manufacturers' instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared as per standard Affymetrix protocol using 10mg of total RNA to start (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_hyb_protocol	Following cRNA fragmentation, cRNA were hybridized to GeneChip RAT230 2.0 arrays and stained using the Affymetrix Fluidics 450 station as per manufacturers instructions (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G
Sample_data_processing	Data was analyzed using Genespring GX 7.3 (Agilent). Values were transformed using RMA. Raw expression values <0.01 were set to 0.01 and the normalization per chip was set to the 50th percentile. Genes were normalized to DMSO control within each biological replicate.
Sample_platform_id	GPL1355
Sample_contact_name	Jason,Scott,Rockel
Sample_contact_email	jsrockel@uwo.ca
Sample_contact_phone	519-661-2111
Sample_contact_laboratory	Dr. Andrew Leask
Sample_contact_department	Anatomy and Cell Biology
Sample_contact_institute	CIHR Group in Skeletal Development and Remodeling
Sample_contact_address	The University of Western Ontario
Sample_contact_city	London
Sample_contact_state	Ontario
Sample_contact_zip/postal_code	N6A 5C1
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359900/suppl/GSM359900.CEL.gz
Sample_series_id	GSE14402
Sample_data_row_count	31099

GSM359901

GPL1355

U0126 and TNF-a-treated chondrocytes, biological replicate 2

Primary chondrocytes derived from the femoral condyles of neonatal rats, pre-treated withU0126 for 30 min followed by TNF-a for 24h.

Strain:Sprague Dawley, Age:neonatal, Tissue:rat femoral condyle, Cell:chondrocytes, Contidion: Cell Culture/In vitro

none

Sample_geo_accession	GSM359901
Sample_status	Public on Jan 14 2009
Sample_submission_date	Jan 13 2009
Sample_last_update_date	Jan 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Chondrocytes were incubated in serum free media overnight. The next day, chondrocytes were treated with DMSO or U0126 for 30 minutes prior to addition of vehicle (BSA) or TNF-a (30 ng/ml) for 24 h.
Sample_growth_protocol_ch1	Chondrocytes were isolated from the femoral condyles of neonatal (one day old) rats and plated onto tissue culture plastic at a density of ~2.5 x 104 cells/cm2. Cells were grown in culture until confluence was reached, typcially 6-7 days. Media used was RPMI-1640 supplimented with 5% fetal bovine serum (FBS), 100 U/ml Penicillin, 100 g/ml streptomycin and 1% HEPES buffer. The media was changed every 3 days.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was collected by Trizol followed by Qiagen Rneasy mini clean-up, as per manufacturers' instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared as per standard Affymetrix protocol using 10mg of total RNA to start (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_hyb_protocol	Following cRNA fragmentation, cRNA were hybridized to GeneChip RAT230 2.0 arrays and stained using the Affymetrix Fluidics 450 station as per manufacturers instructions (Expression Analysis Technical Manual, 2005-2006, Affymetrix)
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G
Sample_data_processing	Data was analyzed using Genespring GX 7.3 (Agilent). Values were transformed using RMA. Raw expression values <0.01 were set to 0.01 and the normalization per chip was set to the 50th percentile. Genes were normalized to DMSO control within each biological replicate.
Sample_platform_id	GPL1355
Sample_contact_name	Jason,Scott,Rockel
Sample_contact_email	jsrockel@uwo.ca
Sample_contact_phone	519-661-2111
Sample_contact_laboratory	Dr. Andrew Leask
Sample_contact_department	Anatomy and Cell Biology
Sample_contact_institute	CIHR Group in Skeletal Development and Remodeling
Sample_contact_address	The University of Western Ontario
Sample_contact_city	London
Sample_contact_state	Ontario
Sample_contact_zip/postal_code	N6A 5C1
Sample_contact_country	Canada
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM359nnn/GSM359901/suppl/GSM359901.CEL.gz
Sample_series_id	GSE14402
Sample_data_row_count	31099

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