Search results for the GEO ID: GSE14429  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM360320 | GPL570 |
|
Human embryonic kidney cells (HEK293) at 0hr, replicate 1
|
Human embryonic kidney cells (HEK293) at 0hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture)
|
| Sample_geo_accession | GSM360320
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360320/suppl/GSM360320.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360321 | GPL570 |
|
Human embryonic kidney cells (HEK293) at 0hr, replicate 2
|
Human embryonic kidney cells (HEK293) at 0hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture)
|
| Sample_geo_accession | GSM360321
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360321/suppl/GSM360321.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360322 | GPL570 |
|
Human embryonic kidney cells (HEK293) at 0hr, replicate 3
|
Human embryonic kidney cells (HEK293) at 0hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture)
|
| Sample_geo_accession | GSM360322
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360322/suppl/GSM360322.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360323 | GPL570 |
|
Human embryonic kidney cells (HEK293) at 0hr, replicate 4
|
Human embryonic kidney cells (HEK293) at 0hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture)
|
| Sample_geo_accession | GSM360323
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360323/suppl/GSM360323.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360324 | GPL570 |
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 1hr, replicate 1
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 1hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture) treated with epoxomicin for 1hr
|
| Sample_geo_accession | GSM360324
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360324/suppl/GSM360324.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360325 | GPL570 |
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 1hr, replicate 2
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 1hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture) treated with epoxomicin for 1hr
|
| Sample_geo_accession | GSM360325
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360325/suppl/GSM360325.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360326 | GPL570 |
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 1hr, replicate 3
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 1hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture) treated with epoxomicin for 1hr
|
| Sample_geo_accession | GSM360326
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360326/suppl/GSM360326.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360327 | GPL570 |
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 1hr, replicate 4
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 1hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture) treated with epoxomicin for 1hr
|
| Sample_geo_accession | GSM360327
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360327/suppl/GSM360327.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360328 | GPL570 |
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 6hr, replicate 1
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 6hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture) treated with epoxomicin for 6hr
|
| Sample_geo_accession | GSM360328
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360328/suppl/GSM360328.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360329 | GPL570 |
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 6hr, replicate 2
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 6hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture) treated with epoxomicin for 6hr
|
| Sample_geo_accession | GSM360329
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360329/suppl/GSM360329.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360330 | GPL570 |
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 6hr, replicate 3
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 6hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture) treated with epoxomicin for 6hr
|
| Sample_geo_accession | GSM360330
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360330/suppl/GSM360330.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
|
GSM360331 | GPL570 |
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 6hr, replicate 4
|
Human embryonic kidney cells (HEK293) treated with epoxomicin for 6hr
|
HEK293 cells
|
gene expression data from human embryonic kidney cells (cell culture) treated with epoxomicin for 6hr
|
| Sample_geo_accession | GSM360331
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Jan 14 2009
| | Sample_last_update_date | Jan 14 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 1 uM epoxomicin is added to HEK293 cells and incubated for 1 and 6 hours before RNA extraction. RNA was extracted from cells without treatment at zero time point as control.
| | Sample_growth_protocol_ch1 | HEK293 cells are maintained in DMEM supplemented with 10% fetal bovine serum in 3% oxygen, 5% carbon dioxide, 37 degrees Celsius humidified incubator.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from HEK293 cells using Qiagen RNeasy Mini Kit according to manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol using Affymetrix’s IVT labeling kit following the manufacturer’s directions.
| | Sample_hyb_protocol | Fragmented RNA was hybridized to Affymetrix HG-U133 plus 2.0 chips for 16 hours, washed, stained on an Affymetrix fluidics station.
| | Sample_scan_protocol | Hybridized chips were scanned using Affymetrix genechip scanner 3000
| | Sample_data_processing | The data were first processed with GCOS1.4 using Affymetrix default analysis settings. The trimmed mean target intensity of each array was arbitrarily set to 1000. Intensities obtained in CHP file generated by GCOS were normalized using an adaptive variance-stabilizing quantile normalization (Munson, P.J., GeneLogic Workshop of Low Level Analysis of Affymetrix GeneChip Data, 2001, software available at http://abs.cit.nih.gov/geneexpression.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Allen,HK,Chang
| | Sample_contact_email | changah@nhlbi.nih.gov
| | Sample_contact_laboratory | Laboratory of Biochemistry
| | Sample_contact_institute | NIH\NHLBI
| | Sample_contact_address | 50 South Drive, Bldg.50, Rm.2347
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM360nnn/GSM360331/suppl/GSM360331.CEL.gz
| | Sample_series_id | GSE14429
| | Sample_data_row_count | 54675
| | |
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