Search results for the GEO ID: GSE14458 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM361045 | GPL1261 |
|
344SQ, sample a1192
|
344SQ
|
344SQ (highly metastatic)
|
344SQ (highly metastatic), sample a1192
|
Sample_geo_accession | GSM361045
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361045/suppl/GSM361045.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361046 | GPL1261 |
|
344SQ, sample a1193
|
344SQ
|
344SQ (highly metastatic)
|
344SQ (highly metastatic), sample a1193
|
Sample_geo_accession | GSM361046
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361046/suppl/GSM361046.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361047 | GPL1261 |
|
344SQ, sample a1194
|
344SQ
|
344SQ (highly metastatic)
|
344SQ (highly metastatic), sample a1194
|
Sample_geo_accession | GSM361047
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361047/suppl/GSM361047.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361048 | GPL1261 |
|
393LN, sample a0930
|
393LN
|
393LN (intermediately metastatic)
|
393LN (intermediately metastatic), sample a0930
|
Sample_geo_accession | GSM361048
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361048/suppl/GSM361048.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361049 | GPL1261 |
|
344SQ, sample a1202
|
344SQ
|
344SQ (highly metastatic)
|
344SQ (highly metastatic), sample a1202
|
Sample_geo_accession | GSM361049
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361049/suppl/GSM361049.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361050 | GPL1261 |
|
393P, sample a1158
|
393P
|
393P (non-metastatic)
|
393P (non-metastatic), sample a1158
|
Sample_geo_accession | GSM361050
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361050/suppl/GSM361050.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361051 | GPL1261 |
|
393LN, sample a0808
|
393LN
|
393LN (intermediately metastatic)
|
393LN (intermediately metastatic), sample a0808
|
Sample_geo_accession | GSM361051
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361051/suppl/GSM361051.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361052 | GPL1261 |
|
393P, sample a1173
|
393P
|
393P (non-metastatic)
|
393P (non-metastatic), sample a1173
|
Sample_geo_accession | GSM361052
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361052/suppl/GSM361052.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361053 | GPL1261 |
|
393LN, sample a0858
|
393LN
|
393LN (intermediately metastatic)
|
393LN (intermediately metastatic), sample a0858
|
Sample_geo_accession | GSM361053
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361053/suppl/GSM361053.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361054 | GPL1261 |
|
393P, sample a1195
|
393P
|
393P (non-metastatic)
|
393P (non-metastatic), sample a1195
|
Sample_geo_accession | GSM361054
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361054/suppl/GSM361054.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361055 | GPL1261 |
|
393LN, sample a0859
|
393LN
|
393LN (intermediately metastatic)
|
393LN (intermediately metastatic), sample a0859
|
Sample_geo_accession | GSM361055
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361055/suppl/GSM361055.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
|
GSM361056 | GPL1261 |
|
393P, sample a1249
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393P
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393P (non-metastatic)
|
393P (non-metastatic), sample a1249
|
Sample_geo_accession | GSM361056
| Sample_status | Public on Nov 10 2009
| Sample_submission_date | Jan 15 2009
| Sample_last_update_date | Nov 10 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_growth_protocol_ch1 | Cell lines from p53R172H∆g/+ K-rasLA1/+ mice were derived from tumor tissues removed at autopsy from two different mice (#344 and #393). The tissues were minced, placed in culture, and passed serially in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), which yielded mass populations of tumor cells derived from primary lung tumors (344P and 393P), mediastinal lymph nodes (344LN and 393LN), and a subcutaneous site (344SQ).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated by the Ambion kit. RNA quality and quantity were evaluated on a NanoDrop spectrophotometer and the Agilent 2100 Bioanalyzer.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on Mouse430 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and invariant set as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10% of intensity values
| Sample_platform_id | GPL1261
| Sample_contact_name | Chad,,Creighton
| Sample_contact_email | creighto@bcm.tmc.edu
| Sample_contact_department | Biostatistics, Ducan Cancer Center
| Sample_contact_institute | Baylor College of Medicine
| Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361056/suppl/GSM361056.CEL.gz
| Sample_series_id | GSE14458
| Sample_series_id | GSE14459
| Sample_data_row_count | 45101
| |
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