Result

Search results for the GEO ID: GSE14469

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM361386

GPL1261

Synovial Sarcoma-like tumor from R-CreER/SSM2 mouse (RCS5)

Tumors generated in a female mouse exposed to one pulse of 0.1mg/gm Tamoxifen at 3 months of age.

Genotype: R-CreER/SSM2. Age: 13 month.

Gene expression data from murine synovial sarcoma-like tumors

Sample_geo_accession	GSM361386
Sample_status	Public on Apr 14 2009
Sample_submission_date	Jan 16 2009
Sample_last_update_date	Apr 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were humanely sacrificed and the tumors were dissected out. Expression of SYT-SSX2 was further confirmed by EGFP mediated fluorosocence of the Tumors detected under a dissecting scope with a GFP filter. Tumors were minced and put it Trizol (invitrogen).
Sample_growth_protocol_ch1	Tumors were generated in R-CreER/SSM2 mice by random and sporadic expression of the synovial sarcoma associated SYT-SSX2 fusion oncogene in various tissues.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol (Invitrogen) extraction of Total RNA was performed and the RNA was purified using the RNeasy kit (Qiagen) using manufacturers' instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared using the One Cycle Target Labeling Control Reagent Kit according to the manufacturer’s specifications (Affymetrix, 900493).
Sample_hyb_protocol	Following fragmentation, 15ug of cRNA were hybridized for 16 hrs. at 45oC on GeneChip Mouse Genome 430 2.0 Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Array data was normalized using the RMA method in the GeneSifter software.
Sample_platform_id	GPL1261
Sample_contact_name	MALAY,,HALDAR
Sample_contact_laboratory	Kenneth M. Murphy
Sample_contact_department	Pathology and Immunology
Sample_contact_institute	Washington University in St. Louis
Sample_contact_address	7751 charing square lane
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63119
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361386/suppl/GSM361386.CEL.gz
Sample_series_id	GSE14469
Sample_data_row_count	45101

GSM361387

GPL1261

Synovial Sarcoma-like tumor from R-CreER/SSM2 mouse (RCS6)

Tumors generated in a male mouse not exposed Tamoxifen.

Genotype: R-CreER/SSM2. Age: 9 month.

Gene expression data from murine synovial sarcoma-like tumors

Sample_geo_accession	GSM361387
Sample_status	Public on Apr 14 2009
Sample_submission_date	Jan 16 2009
Sample_last_update_date	Apr 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were humanely sacrificed and the tumors were dissected out. Expression of SYT-SSX2 was further confirmed by EGFP mediated fluorosocence of the Tumors detected under a dissecting scope with a GFP filter. Tumors were minced and put it Trizol (invitrogen).
Sample_growth_protocol_ch1	Tumors were generated in R-CreER/SSM2 mice by random and sporadic expression of the synovial sarcoma associated SYT-SSX2 fusion oncogene in various tissues.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol (Invitrogen) extraction of Total RNA was performed and the RNA was purified using the RNeasy kit (Qiagen) using manufacturers' instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared using the One Cycle Target Labeling Control Reagent Kit according to the manufacturer’s specifications (Affymetrix, 900493).
Sample_hyb_protocol	Following fragmentation, 15ug of cRNA were hybridized for 16 hrs. at 45oC on GeneChip Mouse Genome 430 2.0 Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Array data was normalized using the RMA method in the GeneSifter software.
Sample_platform_id	GPL1261
Sample_contact_name	MALAY,,HALDAR
Sample_contact_laboratory	Kenneth M. Murphy
Sample_contact_department	Pathology and Immunology
Sample_contact_institute	Washington University in St. Louis
Sample_contact_address	7751 charing square lane
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63119
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361387/suppl/GSM361387.CEL.gz
Sample_series_id	GSE14469
Sample_data_row_count	45101

GSM361388

GPL1261

Synovial Sarcoma-like tumor from R-CreER/SSM2 mouse (RCS8)

Tumors generated in a female mouse exposed to one pulse of 0.1mg/gm Tamoxifen at 3 months of age.

Genotype: R-CreER/SSM2. Age: 10 month.

Gene expression data from murine synovial sarcoma-like tumors

Sample_geo_accession	GSM361388
Sample_status	Public on Apr 14 2009
Sample_submission_date	Jan 16 2009
Sample_last_update_date	Apr 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were humanely sacrificed and the tumors were dissected out. Expression of SYT-SSX2 was further confirmed by EGFP mediated fluorosocence of the Tumors detected under a dissecting scope with a GFP filter. Tumors were minced and put it Trizol (invitrogen).
Sample_growth_protocol_ch1	Tumors were generated in R-CreER/SSM2 mice by random and sporadic expression of the synovial sarcoma associated SYT-SSX2 fusion oncogene in various tissues.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol (Invitrogen) extraction of Total RNA was performed and the RNA was purified using the RNeasy kit (Qiagen) using manufacturers' instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared using the One Cycle Target Labeling Control Reagent Kit according to the manufacturer’s specifications (Affymetrix, 900493).
Sample_hyb_protocol	Following fragmentation, 15ug of cRNA were hybridized for 16 hrs. at 45oC on GeneChip Mouse Genome 430 2.0 Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Array data was normalized using the RMA method in the GeneSifter software.
Sample_platform_id	GPL1261
Sample_contact_name	MALAY,,HALDAR
Sample_contact_laboratory	Kenneth M. Murphy
Sample_contact_department	Pathology and Immunology
Sample_contact_institute	Washington University in St. Louis
Sample_contact_address	7751 charing square lane
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63119
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361388/suppl/GSM361388.CEL.gz
Sample_series_id	GSE14469
Sample_data_row_count	45101

GSM361389

GPL1261

Synovial Sarcoma-like tumor from R-CreER/SSM2 mouse (RCS9)

Tumors generated in a female mouse exposed to one pulse of 0.1mg/gm Tamoxifen at 3 months of age.

Genotype: R-CreER/SSM2. Age 10 months.

Gene expression data from murine synovial sarcoma-like tumors

Sample_geo_accession	GSM361389
Sample_status	Public on Apr 14 2009
Sample_submission_date	Jan 16 2009
Sample_last_update_date	Apr 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were humanely sacrificed and the tumors were dissected out. Expression of SYT-SSX2 was further confirmed by EGFP mediated fluorosocence of the Tumors detected under a dissecting scope with a GFP filter. Tumors were minced and put it Trizol (invitrogen).
Sample_growth_protocol_ch1	Tumors were generated in R-CreER/SSM2 mice by random and sporadic expression of the synovial sarcoma associated SYT-SSX2 fusion oncogene in various tissues.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol (Invitrogen) extraction of Total RNA was performed and the RNA was purified using the RNeasy kit (Qiagen) using manufacturers' instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared using the One Cycle Target Labeling Control Reagent Kit according to the manufacturer’s specifications (Affymetrix, 900493).
Sample_hyb_protocol	Following fragmentation, 15ug of cRNA were hybridized for 16 hrs. at 45oC on GeneChip Mouse Genome 430 2.0 Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Array data was normalized using the RMA method in the GeneSifter software.
Sample_platform_id	GPL1261
Sample_contact_name	MALAY,,HALDAR
Sample_contact_laboratory	Kenneth M. Murphy
Sample_contact_department	Pathology and Immunology
Sample_contact_institute	Washington University in St. Louis
Sample_contact_address	7751 charing square lane
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63119
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361389/suppl/GSM361389.CEL.gz
Sample_series_id	GSE14469
Sample_data_row_count	45101

GSM361390

GPL1261

Synovial Sarcoma-like tumor from R-CreER/SSM2 mouse (RCS10)

Tumors generated in a male mouse not exposed Tamoxifen.

Genotype: R-CreER/SSM2. Age: 10 month.

Gene expression data from murine synovial sarcoma-like tumors

Sample_geo_accession	GSM361390
Sample_status	Public on Apr 14 2009
Sample_submission_date	Jan 16 2009
Sample_last_update_date	Apr 13 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were humanely sacrificed and the tumors were dissected out. Expression of SYT-SSX2 was further confirmed by EGFP mediated fluorosocence of the Tumors detected under a dissecting scope with a GFP filter. Tumors were minced and put it Trizol (invitrogen).
Sample_growth_protocol_ch1	Tumors were generated in R-CreER/SSM2 mice by random and sporadic expression of the synovial sarcoma associated SYT-SSX2 fusion oncogene in various tissues.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol (Invitrogen) extraction of Total RNA was performed and the RNA was purified using the RNeasy kit (Qiagen) using manufacturers' instructions.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared using the One Cycle Target Labeling Control Reagent Kit according to the manufacturer’s specifications (Affymetrix, 900493).
Sample_hyb_protocol	Following fragmentation, 15ug of cRNA were hybridized for 16 hrs. at 45oC on GeneChip Mouse Genome 430 2.0 Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Array data was normalized using the RMA method in the GeneSifter software.
Sample_platform_id	GPL1261
Sample_contact_name	MALAY,,HALDAR
Sample_contact_laboratory	Kenneth M. Murphy
Sample_contact_department	Pathology and Immunology
Sample_contact_institute	Washington University in St. Louis
Sample_contact_address	7751 charing square lane
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63119
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM361nnn/GSM361390/suppl/GSM361390.CEL.gz
Sample_series_id	GSE14469
Sample_data_row_count	45101

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