Search results for the GEO ID: GSE14525 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM363525 | GPL1261 |
|
WT-SB replicate 1
|
Lung; wild type; spontaneous breathing
|
strain: C57BL/6
Gender: male
Age: 8-12 week
Weight: 20-25g
Wild type
|
WT-SB replicate 1
|
Sample_geo_accession | GSM363525
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Spontaneous breathing
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363525/suppl/GSM363525.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363527 | GPL1261 |
|
WT-SB replicate 3
|
Lung; wild type; spontaneous breathing
|
strain: C57BL/6
Gender: male
Age: 8-12 week
Wild type
|
WT-SB replicate 3
|
Sample_geo_accession | GSM363527
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Spontaneous breathing
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363527/suppl/GSM363527.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363528 | GPL1261 |
|
WT-VILI replicate 1
|
Lung; wild type; VILI
|
strain: C57BL/6
Gender: male
Age: 8-12 week
Wild type
|
WT-VILI replicate 1
|
Sample_geo_accession | GSM363528
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | VILI (40 ml/kg tidal volume, 65 breath/minute, positive expiratory pressure 0 cm H2O, 4 hours)
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363528/suppl/GSM363528.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363529 | GPL1261 |
|
WT-VILI replicate 2
|
Lung; wild type; VILI
|
strain: C57BL/6
Gender: male
Age: 8-12 week
Wild type
|
WT-VILI replicate 2
|
Sample_geo_accession | GSM363529
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | VILI (40 ml/kg tidal volume, 65 breath/minute, positive expiratory pressure 0 cm H2O, 4 hours)
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363529/suppl/GSM363529.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363530 | GPL1261 |
|
WT-VILI replicate 3
|
Lung; wild type; VILI
|
strain: C57BL/6
Gender: male
Age: 8-12 week
Wild type
|
WT-VILI replicate 3
|
Sample_geo_accession | GSM363530
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | VILI (40 ml/kg tidal volume, 65 breath/minute, positive expiratory pressure 0 cm H2O, 4 hours)
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363530/suppl/GSM363530.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363531 | GPL1261 |
|
nmMLCK-/- -SB replicate 1
|
Lung; nmMLCK-/-; spontaneous breathing
|
strain: C57BL/6
Gender: male
Age: 8-12 week
nmMLCK-/-
|
nmMLCK-/- -SB replicate 1
|
Sample_geo_accession | GSM363531
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Spontaneous breathing
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363531/suppl/GSM363531.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363532 | GPL1261 |
|
nmMLCK-/- -SB replicate 2
|
Lung; nmMLCK-/-; spontaneous breathing
|
strain: C57BL/6
Gender: male
Age: 8-12 week
nmMLCK-/-
|
nmMLCK-/- -SB replicate 2
|
Sample_geo_accession | GSM363532
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Spontaneous breathing
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363532/suppl/GSM363532.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363533 | GPL1261 |
|
nmMLCK-/- -SB replicate 3
|
Lung; nmMLCK-/-; spontaneous breathing
|
strain: C57BL/6
Gender: male
Age: 8-12 week
nmMLCK-/-
|
nmMLCK-/- -SB replicate 3
|
Sample_geo_accession | GSM363533
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Spontaneous breathing
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363533/suppl/GSM363533.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363534 | GPL1261 |
|
nmMLCK-/- -VILI replicate 1
|
Lung; nmMLCK-/-; VILI
|
strain: C57BL/6
Gender: male
Age: 8-12 week
nmMLCK-/-
|
nmMLCK-/- -VILI replicate 1
|
Sample_geo_accession | GSM363534
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | VILI (40 ml/kg tidal volume, 65 breath/minute, positive expiratory pressure 0 cm H2O, 4 hours)
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363534/suppl/GSM363534.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363535 | GPL1261 |
|
nmMLCK-/- -VILI replicate 2
|
Lung; nmMLCK-/-; VILI
|
strain: C57BL/6
Gender: male
Age: 8-12 week
nmMLCK-/-
|
nmMLCK-/- -VILI replicate 2
|
Sample_geo_accession | GSM363535
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | VILI (40 ml/kg tidal volume, 65 breath/minute, positive expiratory pressure 0 cm H2O, 4 hours)
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363535/suppl/GSM363535.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
|
GSM363536 | GPL1261 |
|
nmMLCK-/- -VILI replicate 3
|
Lung; nmMLCK-/-; VILI
|
strain: C57BL/6
Gender: male
Age: 8-12 week
nmMLCK-/-
|
nmMLCK-/- -VILI replicate 3
|
Sample_geo_accession | GSM363536
| Sample_status | Public on Mar 02 2009
| Sample_submission_date | Jan 22 2009
| Sample_last_update_date | Jan 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | VILI (40 ml/kg tidal volume, 65 breath/minute, positive expiratory pressure 0 cm H2O, 4 hours)
| Sample_growth_protocol_ch1 | standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization
| Sample_platform_id | GPL1261
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM363nnn/GSM363536/suppl/GSM363536.CEL.gz
| Sample_series_id | GSE14525
| Sample_data_row_count | 45037
| |
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