Sample_geo_accession | GSM364436
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Sample_status | Public on May 10 2011
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Sample_submission_date | Jan 25 2009
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Sample_last_update_date | May 10 2011
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Rattus norvegicus
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Sample_taxid_ch1 | 10116
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Sample_treatment_protocol_ch1 | During this time period, cells were treated with either HCl/BSA (control) or 10 ng/ml TGFbeta1 (TGFbeta1) 3 times per week.
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Sample_growth_protocol_ch1 | Adult rat subventricular zone cells were grown under proliferating conditions in the presence of EGF and FGF for 7 days.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Extraction of total RNA was performed with Rneasy Midi Kit (Qiagen) according to the manufacturer's instructions.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Sample_data_processing | The data were analyzed with Affymetrix Gene Chip Operating Software (GCOS 1.2).
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Sample_platform_id | GPL1355
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Sample_contact_name | Dietrich,,Trümbach
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Sample_contact_email | dietrich.truembach@helmholtz-muenchen.de
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Sample_contact_department | Institute for Developmental Genetics
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Sample_contact_institute | German Research Center for Environmental Health
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Sample_contact_address | Ingolstädter Landstraße 1
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Sample_contact_city | München-Neuherberg
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Sample_contact_zip/postal_code | 85764
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Sample_contact_country | Germany
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM364nnn/GSM364436/suppl/GSM364436.CEL.gz
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Sample_series_id | GSE14562
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Sample_data_row_count | 31099
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