Search results for the GEO ID: GSE14612  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM365044 | GPL339 |
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IFNg_treated4hr_mouse_macrophage_pooled_data
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RAW 264.7 mouse macrophage cell line treated with IFNg for 4 hours
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adherent cell line
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gene expression data from macrophages after IFNg-induced activation
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| Sample_geo_accession | GSM365044
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jan 28 2009
| | Sample_last_update_date | Jan 28 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Growth medium was removed and new DMEM was added 1 hour before treatment. Cells treated were treated with IFNg (1000U/ml) or IFNg (1000U/ml) and adenosine (300 uM) for 4 hours.
| | Sample_growth_protocol_ch1 | Cells were seeded in T75 flasks and grown to 80-90% confluency in a humidified environment at 37 degrees C and 5% CO2. Cells were grown in DMEM supplemented with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Qiagen RNeasy kit according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS) Version 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kimberly,E,Barnholt
| | Sample_contact_laboratory | Rutledge Lab
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | 451 E. Health Sciences Drive
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365044/suppl/GSM365044.CEL.gz
| | Sample_series_id | GSE14612
| | Sample_data_row_count | 22690
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GSM365045 | GPL339 |
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IFNg+Ado_treated4hr_mouse_macrophage_pooled_data
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RAW 264.7 mouse macrophage cell line treated with IFNg + adenosine for 4 hours
|
adherent cell line
|
gene expression data from macrophages after IFNg-induced activation plus adenosine treatment
|
| Sample_geo_accession | GSM365045
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jan 28 2009
| | Sample_last_update_date | Jan 28 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Growth medium was removed and new DMEM was added 1 hour before treatment. Cells treated were treated with IFNg (1000U/ml) or IFNg (1000U/ml) and adenosine (300 uM) for 4 hours.
| | Sample_growth_protocol_ch1 | Cells were seeded in T75 flasks and grown to 80-90% confluency in a humidified environment at 37 degrees C and 5% CO2. Cells were grown in DMEM supplemented with 10% FBS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using the Qiagen RNeasy kit according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45 degrees C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Affymetrix GeneChip Operating Software (GCOS) Version 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kimberly,E,Barnholt
| | Sample_contact_laboratory | Rutledge Lab
| | Sample_contact_department | Internal Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | 451 E. Health Sciences Drive
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365045/suppl/GSM365045.CEL.gz
| | Sample_series_id | GSE14612
| | Sample_data_row_count | 22690
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