Search results for the GEO ID: GSE14642  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM365496 | GPL570 |
|
PBMCs_EG_before exercise_01
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 11
before-exercise
|
EG_pre_AG_9788
|
| Sample_geo_accession | GSM365496
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365496/suppl/GSM365496.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365497 | GPL570 |
|
PBMCs_EG_after exercise_01
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 11
after-exercise
|
EG_peak_AG_9788
|
| Sample_geo_accession | GSM365497
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365497/suppl/GSM365497.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365498 | GPL570 |
|
PBMCs_EG_before exercise_02
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 10
before-exercise
|
EG_pre_CN_4649
|
| Sample_geo_accession | GSM365498
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365498/suppl/GSM365498.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365499 | GPL570 |
|
PBMCs_EG_after exercise_02
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 10
after-exercise
|
EG_peak_CN_4649
|
| Sample_geo_accession | GSM365499
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365499/suppl/GSM365499.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365500 | GPL570 |
|
PBMCs_EG_before exercise_03
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 9
before-exercise
|
EG_pre_DS_2467
|
| Sample_geo_accession | GSM365500
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365500/suppl/GSM365500.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365501 | GPL570 |
|
PBMCs_EG_after exercise_03
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 9
after-exercise
|
EG_peak_DS_2467
|
| Sample_geo_accession | GSM365501
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365501/suppl/GSM365501.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365502 | GPL570 |
|
PBMCs_EG_before exercise_04
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 8
before-exercise
|
EG_pre_GI_2177
|
| Sample_geo_accession | GSM365502
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365502/suppl/GSM365502.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365503 | GPL570 |
|
PBMCs_EG_after exercise_04
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 8
after-exercise
|
EG_peak_GI_2177
|
| Sample_geo_accession | GSM365503
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365503/suppl/GSM365503.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365504 | GPL570 |
|
PBMCs_EG_before exercise_05
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 9
before-exercise
|
EG_pre_GJ_8370
|
| Sample_geo_accession | GSM365504
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365504/suppl/GSM365504.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365505 | GPL570 |
|
PBMCs_EG_after exercise_05
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 9
after-exercise
|
EG_peak_GJ_8370
|
| Sample_geo_accession | GSM365505
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365505/suppl/GSM365505.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365506 | GPL570 |
|
PBMCs_EG_before exercise_06
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 10
before-exercise
|
EG_pre_LB_6693
|
| Sample_geo_accession | GSM365506
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365506/suppl/GSM365506.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365507 | GPL570 |
|
PBMCs_EG_after exercise_06
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 10
after-exercise
|
EG_peak_LB_6693
|
| Sample_geo_accession | GSM365507
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365507/suppl/GSM365507.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365508 | GPL570 |
|
PBMCs_EG_before exercise_07
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 10
before-exercise
|
EG_pre_LS_6711
|
| Sample_geo_accession | GSM365508
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365508/suppl/GSM365508.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365509 | GPL570 |
|
PBMCs_EG_after exercise_07
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 10
after-exercise
|
EG_peak_LS_6711
|
| Sample_geo_accession | GSM365509
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365509/suppl/GSM365509.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365510 | GPL570 |
|
PBMCs_EG_before exercise_08
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 9
before-exercise
|
EG_pre_NN_6641
|
| Sample_geo_accession | GSM365510
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365510/suppl/GSM365510.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365511 | GPL570 |
|
PBMCs_EG_after exercise_08
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 9
after-exercise
|
EG_peak_NN_6641
|
| Sample_geo_accession | GSM365511
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365511/suppl/GSM365511.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365512 | GPL570 |
|
PBMCs_EG_before exercise_09
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 9
before-exercise
|
EG_pre_PN_1290
|
| Sample_geo_accession | GSM365512
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365512/suppl/GSM365512.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365513 | GPL570 |
|
PBMCs_EG_after exercise_09
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 9
after-exercise
|
EG_peak_PN_1290
|
| Sample_geo_accession | GSM365513
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365513/suppl/GSM365513.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365514 | GPL570 |
|
PBMCs_EG_before exercise_10
|
Early girls PBMCs before exercise
|
PBMCs, healthy female, age 10
before-exercise
|
EG_pre_SA_1912
|
| Sample_geo_accession | GSM365514
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365514/suppl/GSM365514.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365515 | GPL570 |
|
PBMCs_EG_after exercise_10
|
Early girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 10
after-exercise
|
EG_peak_SA_1912
|
| Sample_geo_accession | GSM365515
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365515/suppl/GSM365515.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365516 | GPL570 |
|
PBMCs_LG_before exercise_11
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 16
before-exercise
|
LG_pre_BB_2205
|
| Sample_geo_accession | GSM365516
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365516/suppl/GSM365516.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365517 | GPL570 |
|
PBMCs_LG_after exercise_11
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 16
after-exercise
|
LG_peak_BB_2205
|
| Sample_geo_accession | GSM365517
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365517/suppl/GSM365517.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365518 | GPL570 |
|
PBMCs_LG_before exercise_12
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 17
before-exercise
|
LG_pre_FS_7955
|
| Sample_geo_accession | GSM365518
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365518/suppl/GSM365518.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365519 | GPL570 |
|
PBMCs_LG_after exercise_12
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 17
after-exercise
|
LG_peak_FS_7955
|
| Sample_geo_accession | GSM365519
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365519/suppl/GSM365519.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365520 | GPL570 |
|
PBMCs_LG_before exercise_13
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 15
before-exercise
|
LG_pre_GR_1886
|
| Sample_geo_accession | GSM365520
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365520/suppl/GSM365520.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365521 | GPL570 |
|
PBMCs_LG_after exercise_13
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 15
after-exercise
|
LG_peak_GR_1886
|
| Sample_geo_accession | GSM365521
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365521/suppl/GSM365521.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365522 | GPL570 |
|
PBMCs_LG_before exercise_14
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 17
before-exercise
|
LG_pre_GT_6962
|
| Sample_geo_accession | GSM365522
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365522/suppl/GSM365522.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365523 | GPL570 |
|
PBMCs_LG_after exercise_14
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 17
after-exercise
|
LG_peak_GT_6962
|
| Sample_geo_accession | GSM365523
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365523/suppl/GSM365523.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365524 | GPL570 |
|
PBMCs_LG_before exercise_15
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 15
before-exercise
|
LG_pre_KK_3400
|
| Sample_geo_accession | GSM365524
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365524/suppl/GSM365524.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365525 | GPL570 |
|
PBMCs_LG_after exercise_15
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 15
after-exercise
|
LG_peak_KK_3400
|
| Sample_geo_accession | GSM365525
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365525/suppl/GSM365525.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365526 | GPL570 |
|
PBMCs_LG_before exercise_16
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 15
before-exercise
|
LG_pre_KM_4779
|
| Sample_geo_accession | GSM365526
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365526/suppl/GSM365526.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365527 | GPL570 |
|
PBMCs_LG_after exercise_16
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 15
after-exercise
|
LG_peak_KM_4779
|
| Sample_geo_accession | GSM365527
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365527/suppl/GSM365527.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365528 | GPL570 |
|
PBMCs_LG_before exercise_17
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 17
before-exercise
|
LG_pre_LC_7433
|
| Sample_geo_accession | GSM365528
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365528/suppl/GSM365528.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365529 | GPL570 |
|
PBMCs_LG_after exercise_17
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 17
after-exercise
|
LG_peak_LC_7433
|
| Sample_geo_accession | GSM365529
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365529/suppl/GSM365529.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365530 | GPL570 |
|
PBMCs_LG_before exercise_18
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 15
before-exercise
|
LG_pre_NB_3869
|
| Sample_geo_accession | GSM365530
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365530/suppl/GSM365530.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365531 | GPL570 |
|
PBMCs_LG_after exercise_18
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 15
after-exercise
|
LG_peak_NB_3869
|
| Sample_geo_accession | GSM365531
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365531/suppl/GSM365531.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365532 | GPL570 |
|
PBMCs_LG_before exercise_19
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 17
before-exercise
|
LG_pre_PN_7156
|
| Sample_geo_accession | GSM365532
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365532/suppl/GSM365532.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365533 | GPL570 |
|
PBMCs_LG_after exercise_19
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 17
after-exercise
|
LG_peak_PN_7156
|
| Sample_geo_accession | GSM365533
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365533/suppl/GSM365533.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365534 | GPL570 |
|
PBMCs_LG_before exercise_20
|
Late girls PBMCs before exercise
|
PBMCs, healthy female, age 17
before-exercise
|
LG_pre_SB_1361
|
| Sample_geo_accession | GSM365534
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365534/suppl/GSM365534.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
|
GSM365535 | GPL570 |
|
PBMCs_LG_after exercise_20
|
Late girls PBMCs after 30 min bout of exercise
|
PBMCs, healthy female, age 17
after-exercise
|
LG_peak_SB_1361
|
| Sample_geo_accession | GSM365535
| | Sample_status | Public on Jun 17 2009
| | Sample_submission_date | Jan 29 2009
| | Sample_last_update_date | Jun 17 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | PBMCs were isolated using OptiPrep® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzol® reagent and purified using the RNeasy Midi columns method (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 4 μg total RNA was used as a template for double‑stranded cDNA synthesis. Single-stranded then double-stranded cDNA was synthesized from the poly A spike-in controls, and mRNA present in the isolated total RNA using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen Corp., Carlsbad, CA), and a T-7-oligo(dT) primer (Integrated DNA Technologies Inc.,Coralville,IA) that contains a T7 RNA polymerase promoter site added to it’s 3' end. A portion of the resulting double-stranded cDNA was used as a template to generate biotin-tagged cRNA from an in vitro transcription reaction (IVT), using the Affymetrix GeneChip® IVT Labeling Kit.
| | Sample_hyb_protocol | 10 µg of fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133+2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450, followed by scanning on a GeneChip® Scanner 3000.
| | Sample_data_processing | The results were quantified and analyzed using GCOS 1.4 software (Affymetrix, Inc.) using default values (Scaling Target Signal Intensity=500; Normalization, All probe sets; Parameters, all set at default values). The microarray data was analyzed using ArrayAssist® version 5.2.2 (STRATAGENE® ) using GC-RMA.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365535/suppl/GSM365535.CEL.gz
| | Sample_series_id | GSE14642
| | Sample_data_row_count | 54675
| | |
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