Search results for the GEO ID: GSE14662  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM365915 | GPL1261 |
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CD4 T sells, Self Antigen, Rep 1
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Self-antigen model (C3HA)
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B10.d2, Male,CFSE-diluted , Self-antigen activated CD4 T cell
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Gene Expression Profile of a T cell responding to self
CDra-TTT-HA-1a-MOE430_2 [C3HA]
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| Sample_geo_accession | GSM365915
| | Sample_status | Public on Jan 31 2009
| | Sample_submission_date | Jan 30 2009
| | Sample_last_update_date | Jan 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HA-specific CD4 T that had undergone antigen specific division in vivo (i.e. CFSE-diluted)cells were harvested from lymph nodes 3-7 days post adoptive transfer. FACSVantage SE cell sorter (BD Biosciences) was used to sort T cells by gating on CFSE-diluted and Thy1.1+ (donor origin) from enriched CD4 T cells
| | Sample_growth_protocol_ch1 | CFSE labeled TCR transgenic CD4 T cells specific for HA were harvested from donor mice, purified using Miltenyi’s magnetically labeled beads, and adoptively transferred into tumor-antigen recognition model (ProHA x TRAMP), Self-antigen recognition model (C3HA), viral-antigen recognition model (VaccHA) activation control, and naïve adoptive transfer control (Nontrangenic) mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Sorted cells were pelleted suspended in Trizol and stored at -80ºC prior to RNA extraction using the Trizol reagent according to the manufacturer's instructions. RNA integrity was analyzed using an Agilent 2100 Bioanalyzer, the RNA 6000 Pico, and Nano Kits (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard labeling following the manufacturers protocol was carried out after cDNA was synthesized using the Nugen FL-Ovation cDNA Biotin Module V2 kit, (http://www.nugeninc.com/pdfs/flbv2_userguide.pdf).
| | Sample_hyb_protocol | Samples were hybridized to separate Affymetrix Mouse 430 Plus2 expression array chips
| | Sample_scan_protocol | Expression array Chips were scanned using Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Relative expression levels were calculated using the MAS5.0 normalization
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Charles,G,Drake
| | Sample_contact_email | drakech@jhmi.edu
| | Sample_contact_phone | (410) 502-7523
| | Sample_contact_fax | (443) 287-4653
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Department of Oncology
| | Sample_contact_institute | Johns Hopkins Sidney Kimmel Comprehensive Cancer Center
| | Sample_contact_address | 1650 Orleans St CRB I #410
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21231
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365915/suppl/GSM365915.CEL.gz
| | Sample_series_id | GSE14662
| | Sample_data_row_count | 45101
| | |
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GSM365916 | GPL1261 |
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CD4 T sells, No Antigen, Rep 1a
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No Antigen Naïve Adoptive transfer control
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B10.d2, Male, CFSE-Undiluted,none-activated CD4 T cell
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Gene Expression Profile of a Naive T cell
CDra-TTT-NT-1a-MOE430_2 [Nontransgenic 1a]
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| Sample_geo_accession | GSM365916
| | Sample_status | Public on Jan 31 2009
| | Sample_submission_date | Jan 30 2009
| | Sample_last_update_date | Jan 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HA-specific CD4 T that had undergone antigen specific division in vivo (i.e. CFSE-diluted)cells were harvested from lymph nodes 3-7 days post adoptive transfer. FACSVantage SE cell sorter (BD Biosciences) was used to sort T cells by gating on CFSE-diluted and Thy1.1+ (donor origin) from enriched CD4 T cells
| | Sample_growth_protocol_ch1 | CFSE labeled TCR transgenic CD4 T cells specific for HA were harvested from donor mice, purified using Miltenyi’s magnetically labeled beads, and adoptively transferred into tumor-antigen recognition model (ProHA x TRAMP), Self-antigen recognition model (C3HA), viral-antigen recognition model (VaccHA) activation control, and naïve adoptive transfer control (Nontrangenic) mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Sorted cells were pelleted suspended in Trizol and stored at -80ºC prior to RNA extraction using the Trizol reagent according to the manufacturer's instructions. RNA integrity was analyzed using an Agilent 2100 Bioanalyzer, the RNA 6000 Pico, and Nano Kits (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard labeling following the manufacturers protocol was carried out after cDNA was synthesized using the Nugen FL-Ovation cDNA Biotin Module V2 kit, (http://www.nugeninc.com/pdfs/flbv2_userguide.pdf).
| | Sample_hyb_protocol | Samples were hybridized to separate Affymetrix Mouse 430 Plus2 expression array chips
| | Sample_scan_protocol | Expression array Chips were scanned using Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Relative expression levels were calculated using the MAS5.0 normalization
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Charles,G,Drake
| | Sample_contact_email | drakech@jhmi.edu
| | Sample_contact_phone | (410) 502-7523
| | Sample_contact_fax | (443) 287-4653
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Department of Oncology
| | Sample_contact_institute | Johns Hopkins Sidney Kimmel Comprehensive Cancer Center
| | Sample_contact_address | 1650 Orleans St CRB I #410
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21231
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365916/suppl/GSM365916.CEL.gz
| | Sample_series_id | GSE14662
| | Sample_data_row_count | 45101
| | |
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GSM365917 | GPL1261 |
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CD4 T sells, No Antigen, Rep 2a
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No Antigen Naïve Adoptive transfer control
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B10.d2, Male, CFSE-Undiluted, none-activated CD4 T cell
|
Gene Expression Profile of a Naive T cell
CDra-TTT-NT-2a-MOE430_2 [Nontransgenic 2a]
|
| Sample_geo_accession | GSM365917
| | Sample_status | Public on Jan 31 2009
| | Sample_submission_date | Jan 30 2009
| | Sample_last_update_date | Jan 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HA-specific CD4 T that had undergone antigen specific division in vivo (i.e. CFSE-diluted)cells were harvested from lymph nodes 3-7 days post adoptive transfer. FACSVantage SE cell sorter (BD Biosciences) was used to sort T cells by gating on CFSE-diluted and Thy1.1+ (donor origin) from enriched CD4 T cells
| | Sample_growth_protocol_ch1 | CFSE labeled TCR transgenic CD4 T cells specific for HA were harvested from donor mice, purified using Miltenyi’s magnetically labeled beads, and adoptively transferred into tumor-antigen recognition model (ProHA x TRAMP), Self-antigen recognition model (C3HA), viral-antigen recognition model (VaccHA) activation control, and naïve adoptive transfer control (Nontrangenic) mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Sorted cells were pelleted suspended in Trizol and stored at -80ºC prior to RNA extraction using the Trizol reagent according to the manufacturer's instructions. RNA integrity was analyzed using an Agilent 2100 Bioanalyzer, the RNA 6000 Pico, and Nano Kits (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard labeling following the manufacturers protocol was carried out after cDNA was synthesized using the Nugen FL-Ovation cDNA Biotin Module V2 kit, (http://www.nugeninc.com/pdfs/flbv2_userguide.pdf).
| | Sample_hyb_protocol | Samples were hybridized to separate Affymetrix Mouse 430 Plus2 expression array chips
| | Sample_scan_protocol | Expression array Chips were scanned using Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Relative expression levels were calculated using the MAS5.0 normalization
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Charles,G,Drake
| | Sample_contact_email | drakech@jhmi.edu
| | Sample_contact_phone | (410) 502-7523
| | Sample_contact_fax | (443) 287-4653
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Department of Oncology
| | Sample_contact_institute | Johns Hopkins Sidney Kimmel Comprehensive Cancer Center
| | Sample_contact_address | 1650 Orleans St CRB I #410
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21231
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365917/suppl/GSM365917.CEL.gz
| | Sample_series_id | GSE14662
| | Sample_data_row_count | 45101
| | |
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GSM365918 | GPL1261 |
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CD4 T sells, Tumor Antigen, Rep 1
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Tumor-antigen model
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B10.d2, Male,CFSE-diluted,Tumor-antigen activated CD4 T cell
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Gene Expression Profile of a T cell responding to Tumor
CDra-TTT-PT-1a-MOE430_2 [ProHAxTRAMP]
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| Sample_geo_accession | GSM365918
| | Sample_status | Public on Jan 31 2009
| | Sample_submission_date | Jan 30 2009
| | Sample_last_update_date | Jan 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HA-specific CD4 T that had undergone antigen specific division in vivo (i.e. CFSE-diluted)cells were harvested from lymph nodes 3-7 days post adoptive transfer. FACSVantage SE cell sorter (BD Biosciences) was used to sort T cells by gating on CFSE-diluted and Thy1.1+ (donor origin) from enriched CD4 T cells
| | Sample_growth_protocol_ch1 | CFSE labeled TCR transgenic CD4 T cells specific for HA were harvested from donor mice, purified using Miltenyi’s magnetically labeled beads, and adoptively transferred into tumor-antigen recognition model (ProHA x TRAMP), Self-antigen recognition model (C3HA), viral-antigen recognition model (VaccHA) activation control, and naïve adoptive transfer control (Nontrangenic) mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Sorted cells were pelleted suspended in Trizol and stored at -80ºC prior to RNA extraction using the Trizol reagent according to the manufacturer's instructions. RNA integrity was analyzed using an Agilent 2100 Bioanalyzer, the RNA 6000 Pico, and Nano Kits (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard labeling following the manufacturers protocol was carried out after cDNA was synthesized using the Nugen FL-Ovation cDNA Biotin Module V2 kit, (http://www.nugeninc.com/pdfs/flbv2_userguide.pdf).
| | Sample_hyb_protocol | Samples were hybridized to separate Affymetrix Mouse 430 Plus2 expression array chips
| | Sample_scan_protocol | Expression array Chips were scanned using Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Relative expression levels were calculated using the MAS5.0 normalization
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Charles,G,Drake
| | Sample_contact_email | drakech@jhmi.edu
| | Sample_contact_phone | (410) 502-7523
| | Sample_contact_fax | (443) 287-4653
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Department of Oncology
| | Sample_contact_institute | Johns Hopkins Sidney Kimmel Comprehensive Cancer Center
| | Sample_contact_address | 1650 Orleans St CRB I #410
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21231
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365918/suppl/GSM365918.CEL.gz
| | Sample_series_id | GSE14662
| | Sample_data_row_count | 45101
| | |
|
GSM365919 | GPL1261 |
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CD4 T sells, Viral Antigen, Rep 1
|
Viral antigen model
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B10.d2, Male, CFSE-diluted,Viral-antigen activated CD4 T cell
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Gene Expression Profile of a T cell responding to Virus
CDra-TTT-VaC-1a-MOE430_2 [VaccHA]
|
| Sample_geo_accession | GSM365919
| | Sample_status | Public on Jan 31 2009
| | Sample_submission_date | Jan 30 2009
| | Sample_last_update_date | Jan 30 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | HA-specific CD4 T that had undergone antigen specific division in vivo (i.e. CFSE-diluted)cells were harvested from lymph nodes 3-7 days post adoptive transfer. FACSVantage SE cell sorter (BD Biosciences) was used to sort T cells by gating on CFSE-diluted and Thy1.1+ (donor origin) from enriched CD4 T cells
| | Sample_growth_protocol_ch1 | CFSE labeled TCR transgenic CD4 T cells specific for HA were harvested from donor mice, purified using Miltenyi’s magnetically labeled beads, and adoptively transferred into tumor-antigen recognition model (ProHA x TRAMP), Self-antigen recognition model (C3HA), viral-antigen recognition model (VaccHA) activation control, and naïve adoptive transfer control (Nontrangenic) mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Sorted cells were pelleted suspended in Trizol and stored at -80ºC prior to RNA extraction using the Trizol reagent according to the manufacturer's instructions. RNA integrity was analyzed using an Agilent 2100 Bioanalyzer, the RNA 6000 Pico, and Nano Kits (Agilent Technologies).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Standard labeling following the manufacturers protocol was carried out after cDNA was synthesized using the Nugen FL-Ovation cDNA Biotin Module V2 kit, (http://www.nugeninc.com/pdfs/flbv2_userguide.pdf).
| | Sample_hyb_protocol | Samples were hybridized to separate Affymetrix Mouse 430 Plus2 expression array chips
| | Sample_scan_protocol | Expression array Chips were scanned using Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Relative expression levels were calculated using the MAS5.0 normalization
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Charles,G,Drake
| | Sample_contact_email | drakech@jhmi.edu
| | Sample_contact_phone | (410) 502-7523
| | Sample_contact_fax | (443) 287-4653
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Department of Oncology
| | Sample_contact_institute | Johns Hopkins Sidney Kimmel Comprehensive Cancer Center
| | Sample_contact_address | 1650 Orleans St CRB I #410
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21231
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM365nnn/GSM365919/suppl/GSM365919.CEL.gz
| | Sample_series_id | GSE14662
| | Sample_data_row_count | 45101
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