Search results for the GEO ID: GSE14807 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM370545 | GPL570 |
|
CMV_1 Control sample, replicate 1
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HEK293 cells
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HEK293 cells, transfected with empty vector, used as controls.
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Intensity values were obtained using the Affymetrix GeneChip Scanner 3000
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Sample_geo_accession | GSM370545
| Sample_status | Public on Sep 28 2010
| Sample_submission_date | Feb 12 2009
| Sample_last_update_date | Sep 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were transfected with pGEX-4T-327 vector. Cells were treated with vehicle and used as controls for other treatments.
| Sample_growth_protocol_ch1 | Cells were grown at 37C/5%CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen), according to manufacture's protocol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | First, cDNA was obtained using the One-Cycle cDNA Synthesis kit. Then, the GeneChip Sample Cleanup Module was used to clean the samples. Finally, biotin-labeled cRNA was obtained using the GeneChip Labeling kit. All kits used were from Affymetrix.
| Sample_hyb_protocol | According to Affymetrix Technical Manual (www.affymetrix.com). cRNA was fragmented prior to hybridization. Samples were incubates at 45C for 16 hours in an hybridization oven. After that, chips were washed and stained with streptavidin. After a washing step, chips were ready to be scanned.
| Sample_scan_protocol | According to Affymetrix Technical Manual (www.affymetrix.com)
| Sample_data_processing | Data were normalized with RMA method using Bioconductor (affyPLM package).
| Sample_platform_id | GPL570
| Sample_contact_name | Trinidad,,Montero
| Sample_contact_email | t.monteromelendez@qmul.ac.uk
| Sample_contact_department | Biochemical Pharmacology
| Sample_contact_institute | Whilliam Harvey Research Institute, Bart's and The London School of Medicine; Queen Mary University of London
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC1M 6BQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM370nnn/GSM370545/suppl/GSM370545.CEL.gz
| Sample_series_id | GSE14807
| Sample_data_row_count | 54675
| |
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GSM370550 | GPL570 |
|
CMV_2 Control sample, replicate 2
|
HEK293 cells
|
HEK293 cells, transfected with empty vector, used as controls.
|
Intensity values were obtained using the Affymetrix GeneChip Scanner 3000
|
Sample_geo_accession | GSM370550
| Sample_status | Public on Sep 28 2010
| Sample_submission_date | Feb 12 2009
| Sample_last_update_date | Sep 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were transfected with pGEX-4T-327 vector. Cells were treated with vehicle and used as controls for other treatments.
| Sample_growth_protocol_ch1 | Cells were grown at 37C/5%CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen), according to manufacture's protocol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | First, cDNA was obtained using the One-Cycle cDNA Synthesis kit. Then, the GeneChip Sample Cleanup Module was used to clean the samples. Finally, biotin-labeled cRNA was obtained using the GeneChip Labeling kit. All kits used were from Affymetrix.
| Sample_hyb_protocol | According to Affymetrix Technical Manual (www.affymetrix.com). cRNA was fragmented prior to hybridization. Samples were incubates at 45C for 16 hours in an hybridization oven. After that, chips were washed and stained with streptavidin. After a washing step, chips were ready to be scanned.
| Sample_scan_protocol | According to Affymetrix Technical Manual (www.affymetrix.com)
| Sample_data_processing | Data were normalized with RMA method using Bioconductor (affyPLM package).
| Sample_platform_id | GPL570
| Sample_contact_name | Trinidad,,Montero
| Sample_contact_email | t.monteromelendez@qmul.ac.uk
| Sample_contact_department | Biochemical Pharmacology
| Sample_contact_institute | Whilliam Harvey Research Institute, Bart's and The London School of Medicine; Queen Mary University of London
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC1M 6BQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM370nnn/GSM370550/suppl/GSM370550.CEL.gz
| Sample_series_id | GSE14807
| Sample_data_row_count | 54675
| |
|
GSM370551 | GPL570 |
|
ALXR_1 replicate 1
|
HEK293 cells
|
Cells were transfected with the annexin receptor ALXR (also called FPR2, formyl peptide receptor 2) in pGEX-4T-327 expression vector.
|
Intensity values were obtained using the Affymetrix GeneChip Scanner 3000
|
Sample_geo_accession | GSM370551
| Sample_status | Public on Sep 28 2010
| Sample_submission_date | Feb 12 2009
| Sample_last_update_date | Sep 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with vehicle and used as controls for other treatments.
| Sample_growth_protocol_ch1 | Cells were grown at 37C/5%CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen), according to manufacture's protocol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | First, cDNA was obtained using the One-Cycle cDNA Synthesis kit. Then, the GeneChip Sample Cleanup Module was used to clean the samples. Finally, biotin-labeled cRNA was obtained using the GeneChip Labeling kit. All kits used were from Affymetrix.
| Sample_hyb_protocol | According to Affymetrix Technical Manual (www.affymetrix.com). cRNA was fragmented prior to hybridization. Samples were incubates at 45C for 16 hours in an hybridization oven. After that, chips were washed and stained with streptavidin. After a washing step, chips were ready to be scanned.
| Sample_scan_protocol | According to Affymetrix Technical Manual (www.affymetrix.com)
| Sample_data_processing | Data were normalized with RMA method using Bioconductor (affyPLM package).
| Sample_platform_id | GPL570
| Sample_contact_name | Trinidad,,Montero
| Sample_contact_email | t.monteromelendez@qmul.ac.uk
| Sample_contact_department | Biochemical Pharmacology
| Sample_contact_institute | Whilliam Harvey Research Institute, Bart's and The London School of Medicine; Queen Mary University of London
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC1M 6BQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM370nnn/GSM370551/suppl/GSM370551.CEL.gz
| Sample_series_id | GSE14807
| Sample_data_row_count | 54675
| |
|
GSM370552 | GPL570 |
|
ALXR_2, replicate 2
|
HEK293 cells
|
HEK293 cells, transfected with ALX receptor (also called FPR2, formyl peptide receptor 2) in pGEX-4T-327 expresson vector.
|
Intensity values were obtained using the Affymetrix GeneChip Scanner 3000
|
Sample_geo_accession | GSM370552
| Sample_status | Public on Sep 28 2010
| Sample_submission_date | Feb 12 2009
| Sample_last_update_date | Sep 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with vehicle and used as controls for other treatments.
| Sample_growth_protocol_ch1 | Cells were grown at 37C/5%CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen), according to manufacture's protocol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | First, cDNA was obtained using the One-Cycle cDNA Synthesis kit. Then, the GeneChip Sample Cleanup Module was used to clean the samples. Finally, biotin-labeled cRNA was obtained using the GeneChip Labeling kit. All kits used were from Affymetrix.
| Sample_hyb_protocol | According to Affymetrix Technical Manual (www.affymetrix.com). cRNA was fragmented prior to hybridization. Samples were incubates at 45C for 16 hours in an hybridization oven. After that, chips were washed and stained with streptavidin. After a washing step, chips were ready to be scanned.
| Sample_scan_protocol | According to Affymetrix Technical Manual (www.affymetrix.com)
| Sample_data_processing | Data were normalized with RMA method using Bioconductor (affyPLM package).
| Sample_platform_id | GPL570
| Sample_contact_name | Trinidad,,Montero
| Sample_contact_email | t.monteromelendez@qmul.ac.uk
| Sample_contact_department | Biochemical Pharmacology
| Sample_contact_institute | Whilliam Harvey Research Institute, Bart's and The London School of Medicine; Queen Mary University of London
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC1M 6BQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM370nnn/GSM370552/suppl/GSM370552.CEL.gz
| Sample_series_id | GSE14807
| Sample_data_row_count | 54675
| |
|
GSM370554 | GPL570 |
|
Ac226 treated cells, replicate 2
|
HEK293 cells
|
HEK293 cells, transfected with ALX receptor (also called FPR2, formyl peptide receptor 2) in pGEX-4T-327 expresson vector and treated with Ac2-26 peptide
|
Intensity values were obtained using the Affymetrix GeneChip Scanner 3000
|
Sample_geo_accession | GSM370554
| Sample_status | Public on Sep 28 2010
| Sample_submission_date | Feb 12 2009
| Sample_last_update_date | Sep 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with 10uM Ac2-26 in PBS for 4 hours.
| Sample_growth_protocol_ch1 | Cells were grown at 37C/5%CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen), according to manufacture's protocol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | First, cDNA was obtained using the One-Cycle cDNA Synthesis kit. Then, the GeneChip Sample Cleanup Module was used to clean the samples. Finally, biotin-labeled cRNA was obtained using the GeneChip Labeling kit. All kits used were from Affymetrix.
| Sample_hyb_protocol | According to Affymetrix Technical Manual (www.affymetrix.com). cRNA was fragmented prior to hybridization. Samples were incubates at 45C for 16 hours in an hybridization oven. After that, chips were washed and stained with streptavidin. After a washing step, chips were ready to be scanned.
| Sample_scan_protocol | According to Affymetrix Technical Manual (www.affymetrix.com)
| Sample_data_processing | Data were normalized with RMA method using Bioconductor (affyPLM package).
| Sample_platform_id | GPL570
| Sample_contact_name | Trinidad,,Montero
| Sample_contact_email | t.monteromelendez@qmul.ac.uk
| Sample_contact_department | Biochemical Pharmacology
| Sample_contact_institute | Whilliam Harvey Research Institute, Bart's and The London School of Medicine; Queen Mary University of London
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC1M 6BQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM370nnn/GSM370554/suppl/GSM370554.CEL.gz
| Sample_series_id | GSE14807
| Sample_data_row_count | 54675
| |
|
GSM370555 | GPL570 |
|
Annexin 1 treated cells, replicate 1
|
HEK293 cells
|
HEK293 cells, transfected with ALX receptor (also called FPR2, formyl peptide receptor 2) in pGEX-4T-327 expresson vector and treated with Annexin 1.
|
Intensity values were obtained using the Affymetrix GeneChip Scanner 3000
|
Sample_geo_accession | GSM370555
| Sample_status | Public on Sep 28 2010
| Sample_submission_date | Feb 12 2009
| Sample_last_update_date | Sep 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with 0.5uM Annexin 1 in PBS for 4 hours.
| Sample_growth_protocol_ch1 | Cells were grown at 37C/5%CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen), according to manufacture's protocol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | First, cDNA was obtained using the One-Cycle cDNA Synthesis kit. Then, the GeneChip Sample Cleanup Module was used to clean the samples. Finally, biotin-labeled cRNA was obtained using the GeneChip Labeling kit. All kits used were from Affymetrix.
| Sample_hyb_protocol | According to Affymetrix Technical Manual (www.affymetrix.com). cRNA was fragmented prior to hybridization. Samples were incubates at 45C for 16 hours in an hybridization oven. After that, chips were washed and stained with streptavidin. After a washing step, chips were ready to be scanned.
| Sample_scan_protocol | According to Affymetrix Technical Manual (www.affymetrix.com)
| Sample_data_processing | Data were normalized with RMA method using Bioconductor (affyPLM package).
| Sample_platform_id | GPL570
| Sample_contact_name | Trinidad,,Montero
| Sample_contact_email | t.monteromelendez@qmul.ac.uk
| Sample_contact_department | Biochemical Pharmacology
| Sample_contact_institute | Whilliam Harvey Research Institute, Bart's and The London School of Medicine; Queen Mary University of London
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC1M 6BQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM370nnn/GSM370555/suppl/GSM370555.CEL.gz
| Sample_series_id | GSE14807
| Sample_data_row_count | 54675
| |
|
GSM370556 | GPL570 |
|
Annexin 1 treated cells, replicate 2
|
HEK293 cells
|
HEK293 cells, transfected with ALX receptor (also called FPR2, formyl peptide receptor 2) in pGEX-4T-327 expresson vector and treated with Annexin 1.
|
Intensity values were obtained using the Affymetrix GeneChip Scanner 3000
|
Sample_geo_accession | GSM370556
| Sample_status | Public on Sep 28 2010
| Sample_submission_date | Feb 12 2009
| Sample_last_update_date | Sep 28 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with 0.5uM Annexin 1 in PBS for 4 hours.
| Sample_growth_protocol_ch1 | Cells were grown at 37C/5%CO2
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol reagent (Invitrogen), according to manufacture's protocol
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | First, cDNA was obtained using the One-Cycle cDNA Synthesis kit. Then, the GeneChip Sample Cleanup Module was used to clean the samples. Finally, biotin-labeled cRNA was obtained using the GeneChip Labeling kit. All kits used were from Affymetrix.
| Sample_hyb_protocol | According to Affymetrix Technical Manual (www.affymetrix.com). cRNA was fragmented prior to hybridization. Samples were incubates at 45C for 16 hours in an hybridization oven. After that, chips were washed and stained with streptavidin. After a washing step, chips were ready to be scanned.
| Sample_scan_protocol | According to Affymetrix Technical Manual (www.affymetrix.com)
| Sample_data_processing | Data were normalized with RMA method using Bioconductor (affyPLM package).
| Sample_platform_id | GPL570
| Sample_contact_name | Trinidad,,Montero
| Sample_contact_email | t.monteromelendez@qmul.ac.uk
| Sample_contact_department | Biochemical Pharmacology
| Sample_contact_institute | Whilliam Harvey Research Institute, Bart's and The London School of Medicine; Queen Mary University of London
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC1M 6BQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM370nnn/GSM370556/suppl/GSM370556.CEL.gz
| Sample_series_id | GSE14807
| Sample_data_row_count | 54675
| |
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