Search results for the GEO ID: GSE14825 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM371102 | GPL570 |
|
RD, rep1
|
RD cells infected with control vector
|
cell line: RD cells
infection: control vector
|
Gene expression data from RD cells infected with control vector.
|
Sample_geo_accession | GSM371102
| Sample_status | Public on Feb 14 2009
| Sample_submission_date | Feb 13 2009
| Sample_last_update_date | Feb 13 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RD cells were retrovirally infected with either MyoD~E heterodimer or an empty control vector, selected for 48 hours in the presence of 1 ug/mL puromycin, and then placed in DMEM media supplemented with 1%Horse serum, Insulin and Transferrin for 24 hours.
| Sample_growth_protocol_ch1 | RD cells were grown in DMEM media supplemented with 10% Bovine Calf Serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were prepared using Qiagen RNeasy Mini Kit according to the manufacture's protocol
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized by RMA using the Affy package of Bioconductor
| Sample_platform_id | GPL570
| Sample_contact_name | Yi,,Cao
| Sample_contact_email | ycao@fhcrc.org
| Sample_contact_phone | 206-667-5278
| Sample_contact_laboratory | Tapscott
| Sample_contact_department | Human Biology
| Sample_contact_institute | Fred Hutchinson CRC
| Sample_contact_address | 1100 Fairview Ave. N
| Sample_contact_city | Seatttle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371102/suppl/GSM371102.CEL.gz
| Sample_series_id | GSE14825
| Sample_data_row_count | 54675
| |
|
GSM371103 | GPL570 |
|
RD, rep2
|
RD cells infected with control vector
|
cell line: RD cells
infection: control vector
|
Gene expression data from RD cells infected with control vector.
|
Sample_geo_accession | GSM371103
| Sample_status | Public on Feb 14 2009
| Sample_submission_date | Feb 13 2009
| Sample_last_update_date | Feb 13 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RD cells were retrovirally infected with either MyoD~E heterodimer or an empty control vector, selected for 48 hours in the presence of 1 ug/mL puromycin, and then placed in DMEM media supplemented with 1%Horse serum, Insulin and Transferrin for 24 hours.
| Sample_growth_protocol_ch1 | RD cells were grown in DMEM media supplemented with 10% Bovine Calf Serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were prepared using Qiagen RNeasy Mini Kit according to the manufacture's protocol
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized by RMA using the Affy package of Bioconductor
| Sample_platform_id | GPL570
| Sample_contact_name | Yi,,Cao
| Sample_contact_email | ycao@fhcrc.org
| Sample_contact_phone | 206-667-5278
| Sample_contact_laboratory | Tapscott
| Sample_contact_department | Human Biology
| Sample_contact_institute | Fred Hutchinson CRC
| Sample_contact_address | 1100 Fairview Ave. N
| Sample_contact_city | Seatttle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371103/suppl/GSM371103.CEL.gz
| Sample_series_id | GSE14825
| Sample_data_row_count | 54675
| |
|
GSM371104 | GPL570 |
|
RD, rep3
|
RD cells infected with control vector
|
cell line: RD cells
infection: control vector
|
Gene expression data from RD cells infected with control vector.
|
Sample_geo_accession | GSM371104
| Sample_status | Public on Feb 14 2009
| Sample_submission_date | Feb 13 2009
| Sample_last_update_date | Feb 13 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RD cells were retrovirally infected with either MyoD~E heterodimer or an empty control vector, selected for 48 hours in the presence of 1 ug/mL puromycin, and then placed in DMEM media supplemented with 1%Horse serum, Insulin and Transferrin for 24 hours.
| Sample_growth_protocol_ch1 | RD cells were grown in DMEM media supplemented with 10% Bovine Calf Serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were prepared using Qiagen RNeasy Mini Kit according to the manufacture's protocol
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized by RMA using the Affy package of Bioconductor
| Sample_platform_id | GPL570
| Sample_contact_name | Yi,,Cao
| Sample_contact_email | ycao@fhcrc.org
| Sample_contact_phone | 206-667-5278
| Sample_contact_laboratory | Tapscott
| Sample_contact_department | Human Biology
| Sample_contact_institute | Fred Hutchinson CRC
| Sample_contact_address | 1100 Fairview Ave. N
| Sample_contact_city | Seatttle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371104/suppl/GSM371104.CEL.gz
| Sample_series_id | GSE14825
| Sample_data_row_count | 54675
| |
|
GSM371105 | GPL570 |
|
RD MD-E, rep1
|
RD cells infected with retrovirus expressing MyoD~E heterodimers
|
cell line: RD cells
infection: retrovirus expressing MyoD~E heterodimers
|
Gene expression data from RD cells infected with retroviral vector expressing MyoD~E heterodimers
|
Sample_geo_accession | GSM371105
| Sample_status | Public on Feb 14 2009
| Sample_submission_date | Feb 13 2009
| Sample_last_update_date | Feb 13 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RD cells were retrovirally infected with either MyoD~E heterodimer or an empty control vector, selected for 48 hours in the presence of 1 ug/mL puromycin, and then placed in DMEM media supplemented with 1%Horse serum, Insulin and Transferrin for 24 hours.
| Sample_growth_protocol_ch1 | RD cells were grown in DMEM media supplemented with 10% Bovine Calf Serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were prepared using Qiagen RNeasy Mini Kit according to the manufacture's protocol
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized by RMA using the Affy package of Bioconductor
| Sample_platform_id | GPL570
| Sample_contact_name | Yi,,Cao
| Sample_contact_email | ycao@fhcrc.org
| Sample_contact_phone | 206-667-5278
| Sample_contact_laboratory | Tapscott
| Sample_contact_department | Human Biology
| Sample_contact_institute | Fred Hutchinson CRC
| Sample_contact_address | 1100 Fairview Ave. N
| Sample_contact_city | Seatttle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371105/suppl/GSM371105.CEL.gz
| Sample_series_id | GSE14825
| Sample_data_row_count | 54675
| |
|
GSM371106 | GPL570 |
|
RD MD-E, rep2
|
RD cells infected with retrovirus expressing MyoD~E heterodimers
|
cell line: RD cells
infection: retrovirus expressing MyoD~E heterodimers
|
Gene expression data from RD cells infected with retroviral vector expressing MyoD~E heterodimers
|
Sample_geo_accession | GSM371106
| Sample_status | Public on Feb 14 2009
| Sample_submission_date | Feb 13 2009
| Sample_last_update_date | Feb 13 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RD cells were retrovirally infected with either MyoD~E heterodimer or an empty control vector, selected for 48 hours in the presence of 1 ug/mL puromycin, and then placed in DMEM media supplemented with 1%Horse serum, Insulin and Transferrin for 24 hours.
| Sample_growth_protocol_ch1 | RD cells were grown in DMEM media supplemented with 10% Bovine Calf Serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were prepared using Qiagen RNeasy Mini Kit according to the manufacture's protocol
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized by RMA using the Affy package of Bioconductor
| Sample_platform_id | GPL570
| Sample_contact_name | Yi,,Cao
| Sample_contact_email | ycao@fhcrc.org
| Sample_contact_phone | 206-667-5278
| Sample_contact_laboratory | Tapscott
| Sample_contact_department | Human Biology
| Sample_contact_institute | Fred Hutchinson CRC
| Sample_contact_address | 1100 Fairview Ave. N
| Sample_contact_city | Seatttle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371106/suppl/GSM371106.CEL.gz
| Sample_series_id | GSE14825
| Sample_data_row_count | 54675
| |
|
GSM371107 | GPL570 |
|
RD MD-E, rep3
|
RD cells infected with retrovirus expressing MyoD~E heterodimers
|
cell line: RD cells
infection: retrovirus expressing MyoD~E heterodimers
|
Gene expression data from RD cells infected with retroviral vector expressing MyoD~E heterodimers
|
Sample_geo_accession | GSM371107
| Sample_status | Public on Feb 14 2009
| Sample_submission_date | Feb 13 2009
| Sample_last_update_date | Feb 13 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | RD cells were retrovirally infected with either MyoD~E heterodimer or an empty control vector, selected for 48 hours in the presence of 1 ug/mL puromycin, and then placed in DMEM media supplemented with 1%Horse serum, Insulin and Transferrin for 24 hours.
| Sample_growth_protocol_ch1 | RD cells were grown in DMEM media supplemented with 10% Bovine Calf Serum.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were prepared using Qiagen RNeasy Mini Kit according to the manufacture's protocol
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized by RMA using the Affy package of Bioconductor
| Sample_platform_id | GPL570
| Sample_contact_name | Yi,,Cao
| Sample_contact_email | ycao@fhcrc.org
| Sample_contact_phone | 206-667-5278
| Sample_contact_laboratory | Tapscott
| Sample_contact_department | Human Biology
| Sample_contact_institute | Fred Hutchinson CRC
| Sample_contact_address | 1100 Fairview Ave. N
| Sample_contact_city | Seatttle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98109
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371107/suppl/GSM371107.CEL.gz
| Sample_series_id | GSE14825
| Sample_data_row_count | 54675
| |
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