Search results for the GEO ID: GSE14844  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM371373 | GPL570 |
|
Blood_TD19901_Dayone_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371373
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371373/suppl/GSM371373.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371374 | GPL570 |
|
Blood_TD19901_Dayone_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371374
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371374/suppl/GSM371374.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371375 | GPL570 |
|
Blood_TD19901_Daytwo_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371375
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371375/suppl/GSM371375.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371376 | GPL570 |
|
Blood_TD19902_Dayone_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371376
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371376/suppl/GSM371376.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371377 | GPL570 |
|
Blood_TD19902_Daytwo_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371377
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371377/suppl/GSM371377.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371378 | GPL570 |
|
Blood_TD19902_Dayone_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371378
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371378/suppl/GSM371378.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371379 | GPL570 |
|
Blood_TD19902_Daytwo_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371379
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371379/suppl/GSM371379.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371380 | GPL570 |
|
Blood_TD23461_Daytwo_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371380
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371380/suppl/GSM371380.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371381 | GPL570 |
|
Blood_TD23461_Daytwo_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371381
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371381/suppl/GSM371381.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371382 | GPL570 |
|
Blood_TD23462_Daytwo_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371382
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371382/suppl/GSM371382.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371383 | GPL570 |
|
Blood_TD24111_Dayone_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371383
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371383/suppl/GSM371383.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371384 | GPL570 |
|
Blood_TD24111_Daytw0_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371384
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371384/suppl/GSM371384.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371385 | GPL570 |
|
Blood_TD24111_Dayone_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371385
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371385/suppl/GSM371385.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371386 | GPL570 |
|
Blood_TD24111_Daytwo_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371386
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371386/suppl/GSM371386.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371387 | GPL570 |
|
Blood_TD24112_Dayone_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371387
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371387/suppl/GSM371387.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371388 | GPL570 |
|
Blood_TD24112_Daytwo_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371388
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371388/suppl/GSM371388.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371389 | GPL570 |
|
Blood_TD24112_Dayone_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371389
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371389/suppl/GSM371389.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371390 | GPL570 |
|
Blood_TD24112_Daytwo_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371390
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371390/suppl/GSM371390.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371391 | GPL570 |
|
Blood_TD23461_Dayone_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371391
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371391/suppl/GSM371391.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371392 | GPL570 |
|
Blood_TD23461_Dayone_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371392
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371392/suppl/GSM371392.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371393 | GPL570 |
|
Blood_TD23462_Dayone_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371393
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371393/suppl/GSM371393.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371394 | GPL570 |
|
Blood_TD23462_Dayone_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371394
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371394/suppl/GSM371394.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371395 | GPL570 |
|
Blood_TD32681_Dayone_10am
|
Whole blood
|
gender: Male
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371395
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371395/suppl/GSM371395.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371396 | GPL570 |
|
Blood_TD32681_Daytwo_10am
|
Whole blood
|
gender: Male
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371396
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371396/suppl/GSM371396.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371397 | GPL570 |
|
Blood_TD32681_Dayone_2pm
|
Whole blood
|
gender: Male
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371397
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371397/suppl/GSM371397.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371398 | GPL570 |
|
Blood_TD32681_Daytwo_2pm
|
Whole blood
|
gender: Male
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371398
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 14 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5μg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5μg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371398/suppl/GSM371398.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371399 | GPL570 |
|
Blood_TD32682_Dayone_10am
|
Whole blood
|
gender: Male
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371399
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371399/suppl/GSM371399.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371400 | GPL570 |
|
Blood_TD32682_Daytwo_2pm
|
Whole blood
|
gender: Male
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371400
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371400/suppl/GSM371400.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371401 | GPL570 |
|
Blood_TD51281_Dayone_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371401
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371401/suppl/GSM371401.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371402 | GPL570 |
|
Blood_TD51281_Daytwo_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371402
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371402/suppl/GSM371402.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371403 | GPL570 |
|
Blood_TD51281_Dayone_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371403
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371403/suppl/GSM371403.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371404 | GPL570 |
|
Blood_TD51281_Daytwo_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371404
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371404/suppl/GSM371404.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371405 | GPL570 |
|
Blood_TD51282_Dayone_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371405
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371405/suppl/GSM371405.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371406 | GPL570 |
|
Blood_TD51282_Daytwo_10am
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371406
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371406/suppl/GSM371406.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371407 | GPL570 |
|
Blood_TD51282_Dayone_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371407
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371407/suppl/GSM371407.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
|
GSM371408 | GPL570 |
|
Blood_TD51282_Daytwo_2pm
|
Whole blood
|
gender: Female
age: 12
tissue: whole blood
|
Venous blood samples were collected using a standard phlebotomy protocol in conjunction with the PAXgene Blood RNA System (Becton & Dickinson, Oxford), which allows the collection, stabilization and transportation of a whole blood cellular RNA sample in a closed evacuated system.
|
| Sample_geo_accession | GSM371408
| | Sample_status | Public on Feb 28 2009
| | Sample_submission_date | Feb 15 2009
| | Sample_last_update_date | Feb 27 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from the PAXgene blood samples using the PAXgene Blood RNA Kit protocol (PreAnalytiX GmbH, Feldbachstrasse, CH-8634 Hombrechtikon).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Expression profiles were generated by hybridizing cRNA derived from 5µg of total RNA to Affymetrix U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA) in accordance with the Affymetrix Eukaryote One-Cycle protocol with integrated globin reduction (see Affymetrix GeneChip Globin-reduction Kit Handbook and Affymetrix GeneChip Expression Analysis technical manual).
| | Sample_label_protocol_ch1 | Total RNA was concentrated (GeneChip blood RNA concentration kit; PN 900585) and 5µg used to generate first-strand cDNA synthesis with integrated globin reduction using peptide nucleic acid (PNA) oligonucleotides in order to block reverse transcription of globin mRNA (GeneChip Globin-Reduction RNA controls; PN 900586, GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents; PN 900493). After second-strand cDNA synthesis, biotinylated cRNA was generated.
| | Sample_hyb_protocol | Biotinylated cRNA was fragmented and hybridized to Affymetrix U133 Plus 2.0 Arrays for 16 hours at 45°C in an Affymetrix hybridization oven 640. Arrays were then washed and stained on an Affymetrix fluidic station 450 (protocol FS450_0001).
| | Sample_scan_protocol | Each array was scanned using an Affymetrix GeneChip Scanner 3000 and GeneChip Operating Software (GCOS) version 1.4 was used to obtain fluorescence intensities
| | Sample_data_processing | The arrays were processed together using Robust Multiarray Average (RMA; [Irizarry et al., 2003]), implemented in the ‘affy’ package in the statistical software environment R (http://www.r-project.org/), to produce normalized, background-adjusted, perfect-match, log-transformed probe set summaries.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Emma,,Meaburn
| | Sample_contact_email | e.meaburn@iop.kcl.ac.uk
| | Sample_contact_laboratory | Lab
| | Sample_contact_department | SGDP
| | Sample_contact_institute | Institute Of Psychiatry
| | Sample_contact_address | PO 82, Denmark Hill
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE5 8AF
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371408/suppl/GSM371408.CEL.gz
| | Sample_series_id | GSE14844
| | Sample_data_row_count | 54675
| | |
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