Search results for the GEO ID: GSE14848 |
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(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM371457 | GPL1355 |
|
Cardiac GATA-4 overexpression, 1
|
Left ventricular gene expression 3 days after LacZ gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371457
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371457/suppl/GSM371457.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371457/suppl/GSM371457.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
|
GSM371458 | GPL1355 |
|
Cardiac GATA-4 overexpression, 2
|
Left ventricular gene expression 3 days after LacZ gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371458
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371458/suppl/GSM371458.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371458/suppl/GSM371458.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
|
GSM371459 | GPL1355 |
|
Cardiac GATA-4 overexpression, 3
|
Left ventricular gene expression 3 days after LacZ gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371459
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371459/suppl/GSM371459.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371459/suppl/GSM371459.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
|
GSM371460 | GPL1355 |
|
Cardiac GATA-4 overexpression, 4
|
Left ventricular gene expression 3 days after LacZ gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371460
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371460/suppl/GSM371460.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371460/suppl/GSM371460.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
|
GSM371461 | GPL1355 |
|
Cardiac GATA-4 overexpression, 5
|
Left ventricular gene expression 3 days after LacZ gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371461
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371461/suppl/GSM371461.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371461/suppl/GSM371461.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
|
GSM371462 | GPL1355 |
|
Cardiac GATA-4 overexpression, 6
|
Left ventricular gene expression 3 days after GATA-4 gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371462
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371462/suppl/GSM371462.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371462/suppl/GSM371462.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
|
GSM371463 | GPL1355 |
|
Cardiac GATA-4 overexpression, 7
|
Left ventricular gene expression 3 days after GATA-4 gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371463
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371463/suppl/GSM371463.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371463/suppl/GSM371463.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
|
GSM371464 | GPL1355 |
|
Cardiac GATA-4 overexpression, 8
|
Left ventricular gene expression 3 days after GATA-4 gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371464
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371464/suppl/GSM371464.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371464/suppl/GSM371464.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
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GSM371635 | GPL1355 |
|
Cardiac GATA-4 overexpression, 9
|
Left ventricular gene expression 3 days after GATA-4 gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371635
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371635/suppl/GSM371635.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371635/suppl/GSM371635.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
|
GSM371636 | GPL1355 |
|
Cardiac GATA-4 overexpression, 10
|
Left ventricular gene expression 3 days after GATA-4 gene transfer
|
strain: Sprague-Dawley
gender: Male
weight: 250-300g
tissue: left ventricle
|
Gene expression profiles three days after GATA-4 gene transfer were compared with those of Lac Z –treated animals by screening Affymetrix Rat Expression Set 230_2.0 Arrays (there are 5 samples in both group).
For additional information: Rysa J, Leskinen H, Ilves M, Ruskoaho H. Hypertension. 2005;45:927-933.
|
Sample_geo_accession | GSM371636
| Sample_status | Public on Dec 01 2009
| Sample_submission_date | Feb 16 2009
| Sample_last_update_date | Jun 26 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from left ventricular samples was isolated by the guanidine thiocyanate-CsCl method.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | After hybridization GeneChips were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Staining signal was amplified by biotinylated anti-streptavidin (Vector Laboratories) and second staining with streptavidin-phycoerythrin by using Affymetrix Fluidics station according the standard protocol.
| Sample_hyb_protocol | Wash A1 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A1 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A1 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A1 Cycle: 2
| Sample_hyb_protocol | Wash B Recovery Mixes: 0
| Sample_hyb_protocol | Wash B Temperature (C): 50
| Sample_hyb_protocol | Number of Wash B Cycles: 6
| Sample_hyb_protocol | Mixes per Wash B Cycle: 15
| Sample_hyb_protocol | Stain Temperature (C): 35
| Sample_hyb_protocol | First Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A2 Recovery Mixes: 0
| Sample_hyb_protocol | Wash A2 Temperature (C): 30
| Sample_hyb_protocol | Number of Wash A2 Cycles: 10
| Sample_hyb_protocol | Mixes per Wash A2 Cycle: 4
| Sample_hyb_protocol | Second Stain Time (seconds): 300
| Sample_hyb_protocol | Third Stain Time (seconds): 300
| Sample_hyb_protocol | Wash A3 Recovery Mixes: 0
| Sample_scan_protocol | GeneChip Scanner 3000 with GeneChip Operating Software (GCOS) v. 1.2 (Affymetrix) was used in scanning.
| Sample_data_processing | GeneChip Operating Software (GCOS) v. 1.2
| Sample_platform_id | GPL1355
| Sample_contact_name | Jaana,,Rysä
| Sample_contact_email | jaana.rysa@oulu.fi
| Sample_contact_phone | +358-8-5375262
| Sample_contact_department | Pharmacology and toxicology
| Sample_contact_institute | University of Oulu
| Sample_contact_address | PO BOX 5000
| Sample_contact_city | University of OULU
| Sample_contact_zip/postal_code | 90014
| Sample_contact_country | Finland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371636/suppl/GSM371636.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM371nnn/GSM371636/suppl/GSM371636.CHP.gz
| Sample_series_id | GSE14848
| Sample_data_row_count | 31099
| |
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