Search results for the GEO ID: GSE14886  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM372018 | GPL570 |
|
doxycycline control
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control HTETOP cells
|
cell line: HTETOP
|
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from cell passage 15, 16, 17 was used for hybridization
|
| Sample_geo_accession | GSM372018
| | Sample_status | Public on Feb 19 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HTETOP cells were treated with 1 µg/ml doxycycline or vehicle for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned with the GeneChip Scanner 3000.
| | Sample_data_processing | The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shiwei,,Deng
| | Sample_contact_email | deng@uni-mainz.de
| | Sample_contact_institute | Uni Mainz
| | Sample_contact_address | Obere Zahlbacher Str. 67
| | Sample_contact_city | Mainz
| | Sample_contact_zip/postal_code | 55101
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372018/suppl/GSM372018.CEL.gz
| | Sample_series_id | GSE14886
| | Sample_data_row_count | 54675
| | |
|
GSM372019 | GPL570 |
|
doxycycline
|
doxycycline-treated HTETOP cells
|
cell line: HTETOP
|
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from cell passage 15, 16, 17 was used for hybridization
|
| Sample_geo_accession | GSM372019
| | Sample_status | Public on Feb 19 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HTETOP cells were treated with 1 µg/ml doxycycline or vehicle for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned with the GeneChip Scanner 3000.
| | Sample_data_processing | The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shiwei,,Deng
| | Sample_contact_email | deng@uni-mainz.de
| | Sample_contact_institute | Uni Mainz
| | Sample_contact_address | Obere Zahlbacher Str. 67
| | Sample_contact_city | Mainz
| | Sample_contact_zip/postal_code | 55101
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372019/suppl/GSM372019.CEL.gz
| | Sample_series_id | GSE14886
| | Sample_data_row_count | 54675
| | |
|
GSM373559 | GPL570 |
|
dexrazoxane control 1
|
control HTETOP cells
|
cell line: HTETOP
|
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from three cell passages was used for hybridization
|
| Sample_geo_accession | GSM373559
| | Sample_status | Public on Feb 24 2009
| | Sample_submission_date | Feb 23 2009
| | Sample_last_update_date | Feb 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HTETOP cells were treated with 100 µM dexrazoxane or vehicle for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Human Genome U133 Plus 2.0 GeneChips from Affymetrix.
| | Sample_scan_protocol | GeneChips were scanned with the GeneChip Scanner 3000.
| | Sample_data_processing | The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shiwei,,Deng
| | Sample_contact_email | deng@uni-mainz.de
| | Sample_contact_institute | Uni Mainz
| | Sample_contact_address | Obere Zahlbacher Str. 67
| | Sample_contact_city | Mainz
| | Sample_contact_zip/postal_code | 55101
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM373nnn/GSM373559/suppl/GSM373559.CEL.gz
| | Sample_series_id | GSE14886
| | Sample_data_row_count | 54675
| | |
|
GSM373560 | GPL570 |
|
dexrazoxane control 2
|
control HTETOP cells
|
cell line: HTETOP
|
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from three cell passages was used for hybridization
|
| Sample_geo_accession | GSM373560
| | Sample_status | Public on Feb 24 2009
| | Sample_submission_date | Feb 23 2009
| | Sample_last_update_date | Feb 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HTETOP cells were treated with 100 µM dexrazoxane or vehicle for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Human Genome U133 Plus 2.0 GeneChips from Affymetrix.
| | Sample_scan_protocol | GeneChips were scanned with the GeneChip Scanner 3000.
| | Sample_data_processing | The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shiwei,,Deng
| | Sample_contact_email | deng@uni-mainz.de
| | Sample_contact_institute | Uni Mainz
| | Sample_contact_address | Obere Zahlbacher Str. 67
| | Sample_contact_city | Mainz
| | Sample_contact_zip/postal_code | 55101
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM373nnn/GSM373560/suppl/GSM373560.CEL.gz
| | Sample_series_id | GSE14886
| | Sample_data_row_count | 54675
| | |
|
GSM373561 | GPL570 |
|
dexrazoxane control 3
|
control HTETOP cells
|
cell line: HTETOP
|
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from three cell passages was used for hybridization
|
| Sample_geo_accession | GSM373561
| | Sample_status | Public on Feb 24 2009
| | Sample_submission_date | Feb 23 2009
| | Sample_last_update_date | Feb 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HTETOP cells were treated with 100 µM dexrazoxane or vehicle for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Human Genome U133 Plus 2.0 GeneChips from Affymetrix.
| | Sample_scan_protocol | GeneChips were scanned with the GeneChip Scanner 3000.
| | Sample_data_processing | The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shiwei,,Deng
| | Sample_contact_email | deng@uni-mainz.de
| | Sample_contact_institute | Uni Mainz
| | Sample_contact_address | Obere Zahlbacher Str. 67
| | Sample_contact_city | Mainz
| | Sample_contact_zip/postal_code | 55101
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM373nnn/GSM373561/suppl/GSM373561.CEL.gz
| | Sample_series_id | GSE14886
| | Sample_data_row_count | 54675
| | |
|
GSM373562 | GPL570 |
|
dexrazoxane 1
|
dexrazoxane-treated HTETOP cells
|
cell line: HTETOP
|
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from three cell passages was used for hybridization
|
| Sample_geo_accession | GSM373562
| | Sample_status | Public on Feb 24 2009
| | Sample_submission_date | Feb 23 2009
| | Sample_last_update_date | Feb 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HTETOP cells were treated with 100 µM dexrazoxane or vehicle for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Human Genome U133 Plus 2.0 GeneChips from Affymetrix.
| | Sample_scan_protocol | GeneChips were scanned with the GeneChip Scanner 3000.
| | Sample_data_processing | The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shiwei,,Deng
| | Sample_contact_email | deng@uni-mainz.de
| | Sample_contact_institute | Uni Mainz
| | Sample_contact_address | Obere Zahlbacher Str. 67
| | Sample_contact_city | Mainz
| | Sample_contact_zip/postal_code | 55101
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM373nnn/GSM373562/suppl/GSM373562.CEL.gz
| | Sample_series_id | GSE14886
| | Sample_data_row_count | 54675
| | |
|
GSM373563 | GPL570 |
|
dexrazoxane 2
|
dexrazoxane-treated HTETOP cells
|
cell line: HTETOP
|
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from three cell passages was used for hybridization
|
| Sample_geo_accession | GSM373563
| | Sample_status | Public on Feb 24 2009
| | Sample_submission_date | Feb 23 2009
| | Sample_last_update_date | Feb 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HTETOP cells were treated with 100 µM dexrazoxane or vehicle for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Human Genome U133 Plus 2.0 GeneChips from Affymetrix.
| | Sample_scan_protocol | GeneChips were scanned with the GeneChip Scanner 3000.
| | Sample_data_processing | The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shiwei,,Deng
| | Sample_contact_email | deng@uni-mainz.de
| | Sample_contact_institute | Uni Mainz
| | Sample_contact_address | Obere Zahlbacher Str. 67
| | Sample_contact_city | Mainz
| | Sample_contact_zip/postal_code | 55101
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM373nnn/GSM373563/suppl/GSM373563.CEL.gz
| | Sample_series_id | GSE14886
| | Sample_data_row_count | 54675
| | |
|
GSM373564 | GPL570 |
|
dexrazoxane 3
|
dexrazoxane-treated HTETOP cells
|
cell line: HTETOP
|
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from three cell passages was used for hybridization
|
| Sample_geo_accession | GSM373564
| | Sample_status | Public on Feb 24 2009
| | Sample_submission_date | Feb 23 2009
| | Sample_last_update_date | Feb 23 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | HTETOP cells were treated with 100 µM dexrazoxane or vehicle for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to Human Genome U133 Plus 2.0 GeneChips from Affymetrix.
| | Sample_scan_protocol | GeneChips were scanned with the GeneChip Scanner 3000.
| | Sample_data_processing | The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shiwei,,Deng
| | Sample_contact_email | deng@uni-mainz.de
| | Sample_contact_institute | Uni Mainz
| | Sample_contact_address | Obere Zahlbacher Str. 67
| | Sample_contact_city | Mainz
| | Sample_contact_zip/postal_code | 55101
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM373nnn/GSM373564/suppl/GSM373564.CEL.gz
| | Sample_series_id | GSE14886
| | Sample_data_row_count | 54675
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