Search results for the GEO ID: GSE14888  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM372030 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, LA fed, biological rep1
|
3T3-L1 adipocyte tissue culture, LA
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372030
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372030/suppl/GSM372030.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372031 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, LA fed, biological rep2
|
3T3-L1 adipocyte tissue culture, LA
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372031
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372031/suppl/GSM372031.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372032 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, LA fed, biological rep3
|
3T3-L1 adipocyte tissue culture, LA
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372032
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372032/suppl/GSM372032.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372033 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, CLA fed, biological rep1
|
3T3-L1 adipocyte tissue culture, CLA
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372033
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372033/suppl/GSM372033.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372034 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, CLA fed, biological rep2
|
3T3-L1 adipocyte tissue culture, CLA
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372034
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372034/suppl/GSM372034.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372035 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, CLA fed, biological rep3
|
3T3-L1 adipocyte tissue culture, CLA
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372035
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372035/suppl/GSM372035.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372036 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, metformin fed, biological rep1
|
3T3-L1 adipocyte tissue culture, metformin
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372036
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372036/suppl/GSM372036.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372037 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, metformin fed, biological rep2
|
3T3-L1 adipocyte tissue culture, metformin
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372037
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372037/suppl/GSM372037.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372038 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, metformin fed, biological rep3
|
3T3-L1 adipocyte tissue culture, metformin
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372038
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372038/suppl/GSM372038.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372039 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, CLA+metformin fed, biological rep1
|
3T3-L1 adipocyte tissue culture, CLA+metformin
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372039
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372039/suppl/GSM372039.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
| | |
|
GSM372040 | GPL1261 |
|
3T3-L1 adipocyte tissue culture, CLA+metformin fed, biological rep2
|
3T3-L1 adipocyte tissue culture, CLA+metformin
|
cell line: 3T3-L1
|
n/a
|
| Sample_geo_accession | GSM372040
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372040/suppl/GSM372040.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
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GSM372041 | GPL1261 |
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3T3-L1 adipocyte tissue culture, CLA+metformin fed, biological rep3
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3T3-L1 adipocyte tissue culture, CLA+metformin
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cell line: 3T3-L1
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n/a
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| Sample_geo_accession | GSM372041
| | Sample_status | Public on Nov 01 2009
| | Sample_submission_date | Feb 18 2009
| | Sample_last_update_date | Feb 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
| | Sample_data_processing | The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jean-Jack,M.,Riethoven
| | Sample_contact_email | jeanjack@unl.edu
| | Sample_contact_laboratory | Bioinformatics Core Research Facility
| | Sample_contact_department | Beadle Center for Biotechnology
| | Sample_contact_institute | University of Nebraska-Lincoln
| | Sample_contact_address | 1901 Vine Street
| | Sample_contact_city | Lincoln
| | Sample_contact_state | NE
| | Sample_contact_zip/postal_code | 68588-0665
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372041/suppl/GSM372041.CEL.gz
| | Sample_series_id | GSE14888
| | Sample_data_row_count | 45101
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