Search results for the GEO ID: GSE14897 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM372142 | GPL570 |
|
fibroblasts_1
|
ATCC cell line CRL2097 (human foreskin fibroblast)
|
cell line: ATCC CRL2097
cell type: human foreskin fibroblasts
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372142
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372142/suppl/GSM372142.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372142/suppl/GSM372142.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372144 | GPL570 |
|
fibroblasts_2
|
ATCC cell line CRL2097 (human foreskin fibroblast)
|
cell line: ATCC CRL2097
cell type: human foreskin fibroblasts
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372144
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372144/suppl/GSM372144.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372144/suppl/GSM372144.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372146 | GPL570 |
|
fibroblasts_3
|
ATCC cell line CRL2097 (human foreskin fibroblast)
|
cell line: ATCC CRL2097
cell type: human foreskin fibroblasts
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372146
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372146/suppl/GSM372146.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372146/suppl/GSM372146.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372147 | GPL570 |
|
H9_d20_hepatic differentiated_1
|
WAO9 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells subjected to a hepatic differentiation protocol
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372147
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Feb 19 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372147/suppl/GSM372147.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372147/suppl/GSM372147.CHP.gz
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372148 | GPL570 |
|
H9_d20_hepatic differentiated_2
|
WAO9 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells subjected to a hepatic differentiation protocol
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372148
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Feb 19 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372148/suppl/GSM372148.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372148/suppl/GSM372148.CHP.gz
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372149 | GPL570 |
|
H9_d20_hepatic differentiated_3
|
WAO9 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells subjected to a hepatic differentiation protocol
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372149
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Feb 19 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372149/suppl/GSM372149.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372149/suppl/GSM372149.CHP.gz
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372150 | GPL570 |
|
H9_undifferentiated_1
|
WAO9 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells, undifferentiated
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372150
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Feb 19 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372150/suppl/GSM372150.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372150/suppl/GSM372150.CHP.gz
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372151 | GPL570 |
|
H9_undifferentiated_2
|
WAO9 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells, undifferentiated
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372151
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Feb 19 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372151/suppl/GSM372151.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372151/suppl/GSM372151.CHP.gz
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372152 | GPL570 |
|
H9_undifferentiated_3
|
WAO9 human embryonic stem cells
|
cell type: WAO9 human embryonic stem cells, undifferentiated
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372152
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Feb 19 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372152/suppl/GSM372152.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372152/suppl/GSM372152.CHP.gz
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372154 | GPL570 |
|
iPS_d20_hepatic differentiated_1
|
induced pluripotent stem cells derived from ATCC cell line CRL2097 (human foreskin fibroblasts)
|
cell line: ATCC CRL2097
cell type: human foreskin fibroblasts subjected to a hepatic differentiation protocol. samples harvested on d20 of protocol
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372154
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372154/suppl/GSM372154.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372154/suppl/GSM372154.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372155 | GPL570 |
|
iPS_d20_hepatic differentiated_2
|
induced pluripotent stem cells derived from ATCC cell line CRL2097 (human foreskin fibroblasts)
|
cell line: ATCC CRL2097
cell type: human foreskin fibroblasts subjected to a hepatic differentiation protocol. samples harvested on d20 of protocol
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372155
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372155/suppl/GSM372155.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372155/suppl/GSM372155.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372156 | GPL570 |
|
iPS_d20_hepatic differentiated_3
|
induced pluripotent stem cells derived from ATCC cell line CRL2097 (human foreskin fibroblasts)
|
cell line: ATCC CRL2097
cell type: human foreskin fibroblasts subjected to a hepatic differentiation protocol. samples harvested on d20 of protocol
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372156
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372156/suppl/GSM372156.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372156/suppl/GSM372156.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372157 | GPL570 |
|
iPS_undifferentiated_1
|
induced pluripotent stem cells derived from ATCC cell line CRL2097 (human foreskin fibroblasts)
|
cell line: ATCC CRL2097
cell type: induced pluripotent stem cells derived from human foreskin fibroblasts
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372157
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372157/suppl/GSM372157.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372157/suppl/GSM372157.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372158 | GPL570 |
|
iPS_undifferentiated_2
|
induced pluripotent stem cells derived from ATCC cell line CRL2097 (human foreskin fibroblasts)
|
cell line: ATCC CRL2097
cell type: induced pluripotent stem cells derived from human foreskin fibroblasts
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372158
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372158/suppl/GSM372158.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372158/suppl/GSM372158.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
GSM372159 | GPL570 |
|
iPS_undifferentiated_3
|
induced pluripotent stem cells derived from ATCC cell line CRL2097 (human foreskin fibroblasts)
|
cell line: ATCC CRL2097
cell type: induced pluripotent stem cells derived from human foreskin fibroblasts
|
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first showed that mouse iPS cells could from a complete liver in mouse embryo (E14.5) including hepatocytes, endothelial cells, sinusoidal cells and resident macrophages. We then designed a highly efficient hepatocyte differentiation protocol using defined factors on human embryonic stem cells (ES cells). This protocol was found to generate more than 80% albumin expressing cells that show hepatic functions and express most of liver genes as shown by microarray analyses. Similar results were obtained when human iPS cells were induced to differentiate following the same procedure.
|
Sample_geo_accession | GSM372159
| Sample_status | Public on Aug 01 2009
| Sample_submission_date | Feb 18 2009
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Rneasy (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix One-Cycle Target Labeling
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Standard Affymetrix protocol
| Sample_data_processing | Standard Affymetrix protocol, standard GCOS data processing produced the values in the accompanying table
| Sample_platform_id | GPL570
| Sample_contact_name | Stephen,A,Duncan
| Sample_contact_email | duncans@mcw.edu
| Sample_contact_phone | 414 456 8602
| Sample_contact_laboratory | Duncan Lab
| Sample_contact_department | Cell Biology
| Sample_contact_institute | Stephen Duncan
| Sample_contact_address | 8701 Watertown Plank Rd
| Sample_contact_city | Milwaukee
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53226
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372159/suppl/GSM372159.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM372nnn/GSM372159/suppl/GSM372159.CHP.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE14897
| Sample_data_row_count | 54675
| |
|
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