Result

Search results for the GEO ID: GSE14987

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM374141

GPL570

vector control, biological rep1

MCF10A

cell line: MCF10A cell type: immortalized cell culture

vector control, biological rep1

Sample_geo_accession	GSM374141
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374141/suppl/GSM374141.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

GSM374142

GPL570

vector control, biological rep2

MCF10A

cell line: MCF10A cell type: immortalized cell culture

vector control, biological rep2

Sample_geo_accession	GSM374142
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374142/suppl/GSM374142.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

GSM374143

GPL570

vector control, biological rep3

MCF10A

cell line: MCF10A cell type: immortalized cell culture

vector control, biological rep3

Sample_geo_accession	GSM374143
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374143/suppl/GSM374143.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

GSM374144

GPL570

ERBB2 over-expression, biological rep1

MCF10A

cell line: MCF10A cell type: immortalized cell culture

ERBB2 over-expression, biological rep1

Sample_geo_accession	GSM374144
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374144/suppl/GSM374144.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

GSM374145

GPL570

ERBB2 over-expression, biological rep2

MCF10A

cell line: MCF10A cell type: immortalized cell culture

ERBB2 over-expression, biological rep2

Sample_geo_accession	GSM374145
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374145/suppl/GSM374145.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

GSM374146

GPL570

ERBB2 over-expression, biological rep3

MCF10A

cell line: MCF10A cell type: immortalized cell culture

ERBB2 over-expression, biological rep3

Sample_geo_accession	GSM374146
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374146/suppl/GSM374146.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

GSM374147

GPL570

EGF stimulation, biological rep1

MCF10A

cell line: MCF10A cell type: immortalized cell culture

EGF stimulation, biological rep1

Sample_geo_accession	GSM374147
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374147/suppl/GSM374147.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

GSM374148

GPL570

EGF stimulation, biological rep2

MCF10A

cell line: MCF10A cell type: immortalized cell culture

EGF stimulation, biological rep2

Sample_geo_accession	GSM374148
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374148/suppl/GSM374148.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

GSM374149

GPL570

EGF stimulation, biological rep3

MCF10A

cell line: MCF10A cell type: immortalized cell culture

EGF stimulation, biological rep3

Sample_geo_accession	GSM374149
Sample_status	Public on Feb 01 2010
Sample_submission_date	Feb 25 2009
Sample_last_update_date	Jan 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The cells were transduced with retroviruses encoding vector control (pBabe) or pBabe-ErbB2. After infection cells were switched to assay medium supplemented with only 2% horse serum and no EGF. For EGF stimulation, cells transduced with empty vector were treated with EGF at 50 ng/ml for 2 hours before harvesting.
Sample_growth_protocol_ch1	MCF10A cells were maintained in DMEM/F12 base media supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 microgram/ml hydrocortisone, 100 ng/ml cholera toxin, 10 microgram/ml insulin and penicillin/streptomycin.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	QIAGEN RNeasy protocol and reagents for extraction of total RNA
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
Sample_hyb_protocol = Microarray	Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Sample_scan_protocol	Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Sample_data_processing	MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
Sample_platform_id	GPL570
Sample_contact_name	Ben,S.,Wittner
Sample_contact_email	wittner.ben@mgh.harvard.edu
Sample_contact_laboratory	Ramaswamy
Sample_contact_department	Center for Cancer Research
Sample_contact_institute	Massachusetts General Hospital
Sample_contact_address	185 Cambridge St.
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	01945
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM374nnn/GSM374149/suppl/GSM374149.CEL.gz
Sample_series_id	GSE14987
Sample_series_id	GSE14990
Sample_data_row_count	54675

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