Search results for the GEO ID: GSE15155  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM378606 | GPL1261 |
|
quiescent satellite cell_adult muscle_rep1
|
purified satellite cells form adult Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: adult (6weeks)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from adult muscle (Ad.1)
|
| Sample_geo_accession | GSM378606
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378606/suppl/GSM378606.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378607 | GPL1261 |
|
quiescent satellite cell_adult muscle_rep2
|
purified satellite cells form adult Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: adult (6weeks)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from adult muscle (Ad.2)
|
| Sample_geo_accession | GSM378607
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378607/suppl/GSM378607.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378608 | GPL1261 |
|
quiescent satellite cell_adult muscle_rep3
|
purified satellite cells form adult Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: adult (6weeks)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from adult muscle (Ad.3)
|
| Sample_geo_accession | GSM378608
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378608/suppl/GSM378608.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378609 | GPL1261 |
|
activated satellite cell_adult regenerating mdx muscle_rep1
|
purified satellite cells form adult Pax3GFP/+:mdx/mdx muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+:mdx/mdx
tissue: diaphragm,pectoralis,abdominal muscles
age: adult (6weeks)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from adult mdx muscle (Ad.mdx1)
|
| Sample_geo_accession | GSM378609
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378609/suppl/GSM378609.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378610 | GPL1261 |
|
activated satellite cell_adult regenerating mdx muscle_rep2
|
purified satellite cells form adult Pax3GFP/+:mdx/mdx muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+:mdx/mdx
tissue: diaphragm,pectoralis,abdominal muscles
age: adult (6weeks)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from adult mdx muscle (Ad.mdx2)
|
| Sample_geo_accession | GSM378610
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378610/suppl/GSM378610.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378611 | GPL1261 |
|
activated satellite cell_adult regenerating mdx muscle_rep3
|
purified satellite cells form adult Pax3GFP/+:mdx/mdx muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+:mdx/mdx
tissue: diaphragm,pectoralis,abdominal muscles
age: adult (6weeks)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from adult mdx muscle (Ad.mdx3)
|
| Sample_geo_accession | GSM378611
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378611/suppl/GSM378611.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378612 | GPL1261 |
|
activated satellite cell_1wk old growing muscle_rep1
|
purified satellite cells form 1wk old Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: young (1week)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from young muscle (1wk.1)
|
| Sample_geo_accession | GSM378612
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378612/suppl/GSM378612.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378613 | GPL1261 |
|
activated satellite cell_1wk old growing muscle_rep2
|
purified satellite cells form 1wk old Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: young (1week)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from young muscle (1wk.2)
|
| Sample_geo_accession | GSM378613
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378613/suppl/GSM378613.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378614 | GPL1261 |
|
activated satellite cell_1wk old growing muscle_rep3
|
purified satellite cells form 1wk old Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal)
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: young (1week)
origin: freshly isolated
|
Gene expression data of freshly isolated satellite cells from young muscle (1wk.3)
|
| Sample_geo_accession | GSM378614
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378614/suppl/GSM378614.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378615 | GPL1261 |
|
activated satellite cell_cultured 3days_rep1
|
purified satellite cells form adult Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal) after three days in culture
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: cultured (3days)
origin: cultured
|
Gene expression data of cultured satellite cells (Ad.cult1)
|
| Sample_geo_accession | GSM378615
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378615/suppl/GSM378615.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
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GSM378616 | GPL1261 |
|
activated satellite cell_cultured 3days_rep2
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purified satellite cells form adult Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal) after three days in culture
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: cultured (3days)
origin: cultured
|
Gene expression data of cultured satellite cells (Ad.cult2)
|
| Sample_geo_accession | GSM378616
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378616/suppl/GSM378616.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
|
GSM378617 | GPL1261 |
|
activated satellite cell_cultured 3days_rep3
|
purified satellite cells form adult Pax3GFP/+ muscles (diaphragm,pectoralis,abdominal) after three days in culture
|
genotype: Pax3GPF/+
tissue: diaphragm,pectoralis,abdominal muscles
age: cultured (3days)
origin: cultured
|
Gene expression data of cultured satellite cells (Ad.cult3)
|
| Sample_geo_accession | GSM378617
| | Sample_status | Public on Jan 04 2010
| | Sample_submission_date | Mar 09 2009
| | Sample_last_update_date | Jan 04 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | satellite cells were obtained by a pool of Pax3GFP positive muscles (diaphragm,pectoralis,abdominal) by enymatic digestion followed by flow cytometry purification using GFP positivity and size and granularity windows.
| | Sample_growth_protocol_ch1 | mice were kept in the animal house of the Institut Pasteur under an ad libitum food and water regimen, cells culture was perfomed in serum rich medium on gelatine-coated plates
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | micro RNAeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the GeneChip Expression Two-Cycle 3′ amplification system Affymetrix protocol from 100 ng total RNA
| | Sample_hyb_protocol | Following fragmentation, 10 mg of cRNA were hybridized for 16 hr at 45C on GeneChip 3' Eukaryotic Arrays Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS_450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner G3000 7G.
| | Sample_data_processing | RMA normalization algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | didier,,montarras
| | Sample_contact_email | didier.montarras@pasteur.fr
| | Sample_contact_phone | +33145688475
| | Sample_contact_fax | +33140613452
| | Sample_contact_laboratory | developmental molecular genetics
| | Sample_contact_department | developmental biology
| | Sample_contact_institute | institut pasteur
| | Sample_contact_address | 25, rue du dr. Roux
| | Sample_contact_city | paris
| | Sample_contact_zip/postal_code | 75015
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378617/suppl/GSM378617.CEL.gz
| | Sample_series_id | GSE15155
| | Sample_data_row_count | 45101
| | |
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