Search results for the GEO ID: GSE15176 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM378816 | GPL570 |
|
H1L ESC (set of 12)
|
Human Embryonic Stem Cells
|
cell type: H1 passage 42 Human Embryonic Stem Cells
|
Human Embryonic Stem Cell line
|
Sample_geo_accession | GSM378816
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Mar 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Lentiviral transduction of fibroblast cells was performed as previously described.
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells (GM03813 and GM03814, Coriell Inst.) were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner.
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378816/suppl/GSM378816.CEL.gz
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378817 | GPL570 |
|
H7 ESC (set of 12)
|
Human Embryonic Stem Cells
|
cell type: H7 passage 73 Human Embryonic Stem Cells
|
Human Embryonic Stem Cell line
|
Sample_geo_accession | GSM378817
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Lentiviral transduction of fibroblast cells was performed as previously described.
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells (GM03813 and GM03814, Coriell Inst.) were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner.
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378817/suppl/GSM378817.CEL.gz
| Sample_relation | Reanalyzed by: GSM500992
| Sample_relation | Reanalyzed by: GSM656450
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378818 | GPL570 |
|
H9 ESC (set of 12)
|
Human Embryonic Stem Cells
|
cell type: H9 passage 50 Human Embryonic Stem Cells
|
Human Embryonic Stem Cell line
|
Sample_geo_accession | GSM378818
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Lentiviral transduction of fibroblast cells was performed as previously described.
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells (GM03813 and GM03814, Coriell Inst.) were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner.
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378818/suppl/GSM378818.CEL.gz
| Sample_relation | Reanalyzed by: GSM500994
| Sample_relation | Reanalyzed by: GSM656451
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378819 | GPL570 |
|
H13B ESC (set of 12)
|
Human Embryonic Stem Cells
|
cell type: H13B passage 43 Human Embryonic Stem Cells
|
Human Embryonic Stem Cell line
|
Sample_geo_accession | GSM378819
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Lentiviral transduction of fibroblast cells was performed as previously described.
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells (GM03813 and GM03814, Coriell Inst.) were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner.
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378819/suppl/GSM378819.CEL.gz
| Sample_relation | Reanalyzed by: GSM500988
| Sample_relation | Reanalyzed by: GSM656448
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378820 | GPL570 |
|
H14A ESC (set of 12)
|
Human Embryonic Stem Cells
|
cell type: H14B passage 61 Human Embryonic Stem Cells
|
Human Embryonic Stem Cell line
|
Sample_geo_accession | GSM378820
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Lentiviral transduction of fibroblast cells was performed as previously described.
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells (GM03813 and GM03814, Coriell Inst.) were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner.
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378820/suppl/GSM378820.CEL.gz
| Sample_relation | Reanalyzed by: GSM500990
| Sample_relation | Reanalyzed by: GSM656449
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378832 | GPL570 |
|
Foreskin Cells Parental Foreskin (set of 12)
|
Foreskin Cells Parental
|
cell type: Foreskin Cells Parental
|
Foreskin_102208
|
Sample_geo_accession | GSM378832
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Mar 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | IPS cells treated with episomal vectors
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378832/suppl/GSM378832.CEL.gz
| Sample_relation | Reanalyzed by: GSM500995
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378833 | GPL570 |
|
iPS cells from episomal vectors (Defined Factor) DF19_9 (set of 12)
|
iPS cells from episomal vectors (Defined Factor)
|
cell type: iPS cells from episomal vectors (Defined Factor)
|
DF19_9_102208
|
Sample_geo_accession | GSM378833
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | IPS cells treated with episomal vectors
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378833/suppl/GSM378833.CEL.gz
| Sample_relation | Reanalyzed by: GSM500996
| Sample_relation | Reanalyzed by: GSM656442
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378834 | GPL570 |
|
iPS cells from episomal vectors (Defined Factor sub-clone) DF19_9_11T (set of 12)
|
iPS cells from episomal vectors (Defined Factor sub-clone)
|
cell type: iPS cells from episomal vectors (Defined Factor sub-clone)
|
DF19_9_11T_010709
|
Sample_geo_accession | GSM378834
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | IPS cells treated with episomal vectors
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378834/suppl/GSM378834.CEL.gz
| Sample_relation | Reanalyzed by: GSM500997
| Sample_relation | Reanalyzed by: GSM656443
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378835 | GPL570 |
|
iPS cells from episomal vectors (Defined Factor sub-clone) DF19_9_7T (set of 12)
|
iPS cells from episomal vectors (Defined Factor sub-clone)
|
cell type: iPS cells from episomal vectors (Defined Factor sub-clone)
|
DF19_9_7T_010709
|
Sample_geo_accession | GSM378835
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | IPS cells treated with episomal vectors
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378835/suppl/GSM378835.CEL.gz
| Sample_relation | Reanalyzed by: GSM656444
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378836 | GPL570 |
|
iPS cells from episomal vectors (Defined Factor sub-clone) DF6_9_12T (set of 12)
|
iPS cells from episomal vectors (Defined Factor sub-clone)
|
cell type: iPS cells from episomal vectors (Defined Factor sub-clone)
|
DF6_9_12T_010709
|
Sample_geo_accession | GSM378836
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Mar 23 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | IPS cells treated with episomal vectors
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378836/suppl/GSM378836.CEL.gz
| Sample_relation | Reanalyzed by: GSM500998
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
| |
|
GSM378837 | GPL570 |
|
iPS cells from episomal vectors (Defined Factor sub-clone) DF6_9_9T (set of 12)
|
iPS cells from episomal vectors (Defined Factor sub-clone)
|
cell type: iPS cells from episomal vectors (Defined Factor sub-clone)
|
DF6_9_9T_010709
|
Sample_geo_accession | GSM378837
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | IPS cells treated with episomal vectors
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378837/suppl/GSM378837.CEL.gz
| Sample_relation | Reanalyzed by: GSM500999
| Sample_relation | Reanalyzed by: GSM656446
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
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GSM378838 | GPL570 |
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iPS cells from episomal vectors (Defined Factor) DF6_9 (set of 12)
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iPS cells from episomal vectors (Defined Factor)
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cell type: iPS cells from episomal vectors (Defined Factor)
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DF6_9_111808
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Sample_geo_accession | GSM378838
| Sample_status | Public on Mar 27 2009
| Sample_submission_date | Mar 10 2009
| Sample_last_update_date | Jan 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | IPS cells treated with episomal vectors
| Sample_growth_protocol_ch1 | iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
| Sample_hyb_protocol | All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner
| Sample_scan_protocol | AFX GC3000 G7 scanner
| Sample_data_processing | RMA
| Sample_platform_id | GPL570
| Sample_contact_name | Victor,,Ruotti
| Sample_contact_email | ruotti@wisc.edu
| Sample_contact_phone | 608-890-0182
| Sample_contact_fax | 608-890-0167
| Sample_contact_laboratory | Thomson's Lab
| Sample_contact_department | Biotechnology
| Sample_contact_institute | UW Madison
| Sample_contact_address | 425 Henry Mall
| Sample_contact_city | Madison
| Sample_contact_state | WI
| Sample_contact_zip/postal_code | 53706
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM378nnn/GSM378838/suppl/GSM378838.CEL.gz
| Sample_relation | Reanalyzed by: GSM501000
| Sample_relation | Reanalyzed by: GSM656445
| Sample_series_id | GSE15148
| Sample_series_id | GSE15176
| Sample_data_row_count | 54675
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