Result

Search results for the GEO ID: GSE15192

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM379269

GPL570

CD44+/CD24- cells rep 1

immortalized breast epithelial cell line

tissue: Breast epithelial immortalized cell line cell surface markers: CD44 positive, CD24 negative

CD44+/CD24- cells rep 1

Sample_geo_accession	GSM379269
Sample_status	Public on Mar 01 2010
Sample_submission_date	Mar 11 2009
Sample_last_update_date	Mar 11 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were stained with CD44-FITC and CD24-PE antibodies and CD44+/CD24- and CD44-/CD24+ cells were collected by flow cytometry sorting. Sorted cells were plated and grown for 4 days
Sample_growth_protocol_ch1	Cells were maintained in DMEM/F12 with 5% horse serum, 10 microgram/ml Insulin, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.5 microgram/ml hydrocortisone.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen Rneasy
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	Scanned using Affymetrix GeneChip® Scanner 3000 7G
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
Sample_platform_id	GPL570
Sample_contact_name	Harikrishna,,Nakshatri
Sample_contact_department	General Surgery
Sample_contact_institute	Indiana University School of Medicine
Sample_contact_address	1044 W. Walnut St. R4-202
Sample_contact_city	Indianapolis
Sample_contact_state	IN
Sample_contact_zip/postal_code	46202
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379269/suppl/GSM379269.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379269/suppl/GSM379269.CHP.gz
Sample_series_id	GSE15192
Sample_data_row_count	54675

GSM379270

GPL570

CD44+/CD24- cells rep 2

immortalized breast epithelial cell line

tissue: Breast epithelial immortalized cell line cell surface markers: CD44 positive, CD24 negative

CD44+/CD24- cells rep 2

Sample_geo_accession	GSM379270
Sample_status	Public on Mar 01 2010
Sample_submission_date	Mar 11 2009
Sample_last_update_date	Mar 11 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were stained with CD44-FITC and CD24-PE antibodies and CD44+/CD24- and CD44-/CD24+ cells were collected by flow cytometry sorting. Sorted cells were plated and grown for 4 days
Sample_growth_protocol_ch1	Cells were maintained in DMEM/F12 with 5% horse serum, 10 microgram/ml Insulin, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.5 microgram/ml hydrocortisone.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen Rneasy
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	Scanned using Affymetrix GeneChip® Scanner 3000 7G
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
Sample_platform_id	GPL570
Sample_contact_name	Harikrishna,,Nakshatri
Sample_contact_department	General Surgery
Sample_contact_institute	Indiana University School of Medicine
Sample_contact_address	1044 W. Walnut St. R4-202
Sample_contact_city	Indianapolis
Sample_contact_state	IN
Sample_contact_zip/postal_code	46202
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379270/suppl/GSM379270.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379270/suppl/GSM379270.CHP.gz
Sample_series_id	GSE15192
Sample_data_row_count	54675

GSM379271

GPL570

CD44+/CD24- cells rep 3

immortalized breast epithelial cell line

tissue: Breast epithelial immortalized cell line cell surface markers: CD44 positive, CD24 negative

CD44+/CD24- cells rep 3

Sample_geo_accession	GSM379271
Sample_status	Public on Mar 01 2010
Sample_submission_date	Mar 11 2009
Sample_last_update_date	Mar 11 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were stained with CD44-FITC and CD24-PE antibodies and CD44+/CD24- and CD44-/CD24+ cells were collected by flow cytometry sorting. Sorted cells were plated and grown for 4 days
Sample_growth_protocol_ch1	Cells were maintained in DMEM/F12 with 5% horse serum, 10 microgram/ml Insulin, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.5 microgram/ml hydrocortisone.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen Rneasy
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	Scanned using Affymetrix GeneChip® Scanner 3000 7G
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
Sample_platform_id	GPL570
Sample_contact_name	Harikrishna,,Nakshatri
Sample_contact_department	General Surgery
Sample_contact_institute	Indiana University School of Medicine
Sample_contact_address	1044 W. Walnut St. R4-202
Sample_contact_city	Indianapolis
Sample_contact_state	IN
Sample_contact_zip/postal_code	46202
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379271/suppl/GSM379271.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379271/suppl/GSM379271.CHP.gz
Sample_series_id	GSE15192
Sample_data_row_count	54675

GSM379272

GPL570

CD44+/CD24- cells rep 4

immortalized breast epithelial cell line

tissue: Breast epithelial immortalized cell line cell surface markers: CD44 positive, CD24 negative

CD44+/CD24- cells rep 4

Sample_geo_accession	GSM379272
Sample_status	Public on Mar 01 2010
Sample_submission_date	Mar 11 2009
Sample_last_update_date	Mar 11 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were stained with CD44-FITC and CD24-PE antibodies and CD44+/CD24- and CD44-/CD24+ cells were collected by flow cytometry sorting. Sorted cells were plated and grown for 4 days
Sample_growth_protocol_ch1	Cells were maintained in DMEM/F12 with 5% horse serum, 10 microgram/ml Insulin, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.5 microgram/ml hydrocortisone.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen Rneasy
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	Scanned using Affymetrix GeneChip® Scanner 3000 7G
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
Sample_platform_id	GPL570
Sample_contact_name	Harikrishna,,Nakshatri
Sample_contact_department	General Surgery
Sample_contact_institute	Indiana University School of Medicine
Sample_contact_address	1044 W. Walnut St. R4-202
Sample_contact_city	Indianapolis
Sample_contact_state	IN
Sample_contact_zip/postal_code	46202
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379272/suppl/GSM379272.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379272/suppl/GSM379272.CHP.gz
Sample_series_id	GSE15192
Sample_data_row_count	54675

GSM379273

GPL570

CD44-/CD24+ cells rep 1

immortalized breast epithelial cell line

tissue: Breast epithelial immortalized cell line cell surface markers: CD44 negative, CD24 positive

CD44-/CD24+ cells rep 1

Sample_geo_accession	GSM379273
Sample_status	Public on Mar 01 2010
Sample_submission_date	Mar 11 2009
Sample_last_update_date	Mar 11 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were stained with CD44-FITC and CD24-PE antibodies and CD44+/CD24- and CD44-/CD24+ cells were collected by flow cytometry sorting. Sorted cells were plated and grown for 4 days
Sample_growth_protocol_ch1	Cells were maintained in DMEM/F12 with 5% horse serum, 10 microgram/ml Insulin, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.5 microgram/ml hydrocortisone.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen Rneasy
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	Scanned using Affymetrix GeneChip® Scanner 3000 7G
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
Sample_platform_id	GPL570
Sample_contact_name	Harikrishna,,Nakshatri
Sample_contact_department	General Surgery
Sample_contact_institute	Indiana University School of Medicine
Sample_contact_address	1044 W. Walnut St. R4-202
Sample_contact_city	Indianapolis
Sample_contact_state	IN
Sample_contact_zip/postal_code	46202
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379273/suppl/GSM379273.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379273/suppl/GSM379273.CHP.gz
Sample_series_id	GSE15192
Sample_data_row_count	54675

GSM379274

GPL570

CD44-/CD24+ cells rep 2

immortalized breast epithelial cell line

tissue: Breast epithelial immortalized cell line cell surface markers: CD44 negative, CD24 positive

CD44-/CD24+ cells rep 2

Sample_geo_accession	GSM379274
Sample_status	Public on Mar 01 2010
Sample_submission_date	Mar 11 2009
Sample_last_update_date	Mar 11 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were stained with CD44-FITC and CD24-PE antibodies and CD44+/CD24- and CD44-/CD24+ cells were collected by flow cytometry sorting. Sorted cells were plated and grown for 4 days
Sample_growth_protocol_ch1	Cells were maintained in DMEM/F12 with 5% horse serum, 10 microgram/ml Insulin, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.5 microgram/ml hydrocortisone.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen Rneasy
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	Scanned using Affymetrix GeneChip® Scanner 3000 7G
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
Sample_platform_id	GPL570
Sample_contact_name	Harikrishna,,Nakshatri
Sample_contact_department	General Surgery
Sample_contact_institute	Indiana University School of Medicine
Sample_contact_address	1044 W. Walnut St. R4-202
Sample_contact_city	Indianapolis
Sample_contact_state	IN
Sample_contact_zip/postal_code	46202
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379274/suppl/GSM379274.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379274/suppl/GSM379274.CHP.gz
Sample_series_id	GSE15192
Sample_data_row_count	54675

GSM379275

GPL570

CD44-/CD24+ cells rep 3

immortalized breast epithelial cell line

tissue: Breast epithelial immortalized cell line cell surface markers: CD44 negative, CD24 positive

CD44-/CD24+ cells rep 3

Sample_geo_accession	GSM379275
Sample_status	Public on Mar 01 2010
Sample_submission_date	Mar 11 2009
Sample_last_update_date	Mar 11 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were stained with CD44-FITC and CD24-PE antibodies and CD44+/CD24- and CD44-/CD24+ cells were collected by flow cytometry sorting. Sorted cells were plated and grown for 4 days
Sample_growth_protocol_ch1	Cells were maintained in DMEM/F12 with 5% horse serum, 10 microgram/ml Insulin, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.5 microgram/ml hydrocortisone.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen Rneasy
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	Scanned using Affymetrix GeneChip® Scanner 3000 7G
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
Sample_platform_id	GPL570
Sample_contact_name	Harikrishna,,Nakshatri
Sample_contact_department	General Surgery
Sample_contact_institute	Indiana University School of Medicine
Sample_contact_address	1044 W. Walnut St. R4-202
Sample_contact_city	Indianapolis
Sample_contact_state	IN
Sample_contact_zip/postal_code	46202
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379275/suppl/GSM379275.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379275/suppl/GSM379275.CHP.gz
Sample_series_id	GSE15192
Sample_data_row_count	54675

GSM379276

GPL570

CD44-/CD24+ cells rep 4

immortalized breast epithelial cell line

tissue: Breast epithelial immortalized cell line cell surface markers: CD44 negative, CD24 positive

CD44-/CD24+ cells rep 4

Sample_geo_accession	GSM379276
Sample_status	Public on Mar 01 2010
Sample_submission_date	Mar 11 2009
Sample_last_update_date	Mar 11 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were stained with CD44-FITC and CD24-PE antibodies and CD44+/CD24- and CD44-/CD24+ cells were collected by flow cytometry sorting. Sorted cells were plated and grown for 4 days
Sample_growth_protocol_ch1	Cells were maintained in DMEM/F12 with 5% horse serum, 10 microgram/ml Insulin, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.5 microgram/ml hydrocortisone.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen Rneasy
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the Affymetrix standard single cycle 3' IVT protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C onto Human HGU133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	Scanned using Affymetrix GeneChip® Scanner 3000 7G
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
Sample_platform_id	GPL570
Sample_contact_name	Harikrishna,,Nakshatri
Sample_contact_department	General Surgery
Sample_contact_institute	Indiana University School of Medicine
Sample_contact_address	1044 W. Walnut St. R4-202
Sample_contact_city	Indianapolis
Sample_contact_state	IN
Sample_contact_zip/postal_code	46202
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379276/suppl/GSM379276.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM379nnn/GSM379276/suppl/GSM379276.CHP.gz
Sample_series_id	GSE15192
Sample_data_row_count	54675

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